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公开(公告)号：US20240018190A1
公开(公告)日：2024-01-18
申请号：US17373763
申请日：2020-01-10
IPC分类号： C07K14/00 , G01N27/327 , C12Q1/6869
CPC分类号： C07K14/001 , G01N27/3278 , C12Q1/6869 , C07K2319/43 , C07K2319/50
摘要： In various embodiments, a synthetic peptide finding use as a molecular wire in a molecular electronic circuit comprises an alpha helical segment further comprising repeating alpha-helical motifs. The synthetic peptide may further comprise at least one specific conjugation site between the termini for attachment to a molecule such as a binding probe, and may further comprise termini having metal binding functionality such as repeats of material binding sequences. In various aspects, the synthetic peptide comprises intramolecular hydrogen bonding, salt bridges, and optionally, aromatic rings that provide for electrical conductivity through the peptide.
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公开(公告)号：US20220252542A1
公开(公告)日：2022-08-11
申请号：US17575551
申请日：2022-01-13
发明人： Barry Merriman , Sonal Jain , Drew Kennedy , Venkatesh Alagarswamy Govindaraj , Calvin Gardner , Chulmin Choi , Namseok Park , Richard Dubielzig , Le Zheng , David Porras , Paul Pan
IPC分类号： G01N27/447 , B03C5/00
摘要： The disclosed embodiments relate to nanotechnology and to nano-electronics and molecular electronic sensors. In an exemplary embodiment, a nano-sensor having a nanoparticle complex attached at each end to a respective nano-electrode. An exemplary nanoparticle complex includes a biomolecule coupled at each end to a metallic nanoparticle to form a dumbbell-shaped molecular bridge. A method to manufacture single molecule dumbbell nanowires for forming conductive molecular bridges includes the steps of: providing a double-stranded nucleic acid with terminal 3′ thiol modification on both the strands conjugated to a gold (Au) nanoparticle (AuNP) on each end; purifying single biomolecule dumbbells from aggregates using size-exclusion chromatography; imaging the eluted products by electron microscopy to validate formation of single molecule dumbbells; trapping a single molecule dumbbell between a pair of nanoelectrodes on a substrate, the electrodes separated by a nanogap; and measuring the conductivity of a trapped single molecule dumbbell.
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公开(公告)号：US20220148682A1
公开(公告)日：2022-05-12
申请号：US17374973
申请日：2021-07-13
发明人： Barry Merriman , Tim Geiser , Paul Mola , Gina Costa
摘要： In various embodiments, amplification-free DNA information methods, apparatus and systems are disclosed. A method of amplification-free information storage and retrieval comprises encoding digital data such as binary into nucleotide sequence motifs using an encoding scheme, and synthesizing replicate DNA molecules using an amplification-free DNA writing process. The amplification-free process of decoding the information stored in the DNA comprises exposing at least one of the replicate DNA molecules to a molecular electronics sensor that generates distinguishable signals in a measured electrical parameter of the sensor, wherein the distinguishable signals correspond to the sequence motifs, providing decoding back to the digital data.
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公开(公告)号：US20220018798A1
公开(公告)日：2022-01-20
申请号：US17483710
申请日：2021-09-23
IPC分类号： G01N27/327 , C12Q1/6825 , G01N33/543
摘要： In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and a binding probe molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the binding probe. In various examples, the binding probe is an antibody, the Fab domain of an antibody, a protein, a nucleic acid oligomer hybridization probe, or an aptamer. The circuit may further comprise molecular arms used to wire the binding probe to the electrodes. In various embodiments, the circuit functions as a sensor wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as targets interact with the binding probe. In various embodiments, the circuit provides a means to measure the presence, absence, or concentration of an analyte in a solution.
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公开(公告)号：US20220002777A1
公开(公告)日：2022-01-06
申请号：US17138314
申请日：2020-12-30
IPC分类号： C12Q1/00 , C12Q1/6825 , G01N33/543 , C12Q1/26 , C07H21/02 , C12Q1/48 , C12Q1/6869
摘要： In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.
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公开(公告)号：US11100404B2
公开(公告)日：2021-08-24
申请号：US16652672
申请日：2018-10-10
发明人： Barry Merriman , Tim Geiser , Paul Mola , Gina Costa
IPC分类号： G06N3/12 , G16B50/30 , G16B30/20 , G16B50/40 , C12N15/11 , C12Q1/6869 , C12Q1/6825
摘要： In various embodiments, amplification-free DNA information methods, apparatus and systems are disclosed. A method of amplification-free information storage and retrieval comprises encoding digital data such as binary into nucleotide sequence motifs using an encoding scheme, and synthesizing replicate DNA molecules using an amplification-free DNA writing process. The amplification-free process of decoding the information stored in the DNA comprises exposing at least one of the replicate DNA molecules to a molecular electronics sensor that generates distinguishable signals in a measured electrical parameter of the sensor, wherein the distinguishable signals correspond to the sequence motifs, providing decoding back to the digital data.
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公开(公告)号：US10902939B2
公开(公告)日：2021-01-26
申请号：US16477106
申请日：2018-01-10
发明人： Barry L. Merriman , Tim Geiser , Paul Mola
IPC分类号： G16B50/40 , C12Q1/6869 , G01N27/327
摘要： In various embodiments, an information storage system comprises: a writing device for synthesizing a nucleotide sequence that encodes a set of information; and a reading device for interpreting the nucleotide sequence by decoding the interpreted nucleotide sequence into the set of information, wherein the reading device comprises a molecular electronics sensor, the sensor comprising a pair of spaced apart electrodes and a molecular complex attached to each electrode to form a molecular electronics circuit, wherein the molecular complex comprises a bridge molecule and a probe molecule, and wherein the molecular electronics sensor produces distinguishable signals in a measurable electrical parameter of the molecular electronics sensor, when interpreting the nucleotide sequence.
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公开(公告)号：US10712334B2
公开(公告)日：2020-07-14
申请号：US16073706
申请日：2017-01-27
发明人： Chulmin Choi , Sungho Jin , Paul W. Mola , Barry L. Merriman
IPC分类号： G01N33/487 , H01M4/92 , H01M4/94 , H01M4/02
摘要： A DNA or genome sequencing structure is disclosed. The structure includes an electrode pair, each electrode having a tip-shaped end, the electrodes separated by a nanogap defined by facing tip-shaped ends; at least one conductive island deposited at or near each tip-shaped end; and a biomolecule having two ends, each end attached to the conductive islands in the electrode pair such that one biomolecule bridges over the nanogap in the electrode pair, wherein nucleotide interactions with the biomolecule provides electronic monitoring of DNA or genome sequencing without the use of a fluorescing element.
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公开(公告)号：US20190194801A1
公开(公告)日：2019-06-27
申请号：US16250929
申请日：2019-01-17
发明人： Sungho Jin , Barry L. Merriman , TIm Geiser , Chulmin Choi , Paul Mola
IPC分类号： C23C16/01 , G01N27/414 , G01N27/04 , G01N27/22
摘要： A molecular sensor includes a substrate defining a substrate plane, and a plurality of pairs of electrode sheets above or below the substrate at an angle to the substrate plane. The molecular sensor further includes a plurality of inner dielectric sheets between each electrode sheet in each pair of electrode sheets of the plurality of pairs, and an outer dielectric sheet between each pair of electrode sheets of the plurality of pairs.
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公开(公告)号：US20190041378A1
公开(公告)日：2019-02-07
申请号：US16073706
申请日：2017-01-27
发明人： Chulmin Choi , Sungho Jin , Paul W. Mola , Barry L. Merriman
IPC分类号： G01N33/487 , H01M4/92 , H01M4/94
摘要： A DNA or genome sequencing structure is disclosed. The structure includes an electrode pair, each electrode having a tip-shaped end, the electrodes separated by a nanogap defined by facing tip-shaped ends; at least one conductive island deposited at or near each tip-shaped end; and a biomolecule having two ends, each end attached to the conductive islands in the electrode pair such that one biomolecule bridges over the nanogap in the electrode pair, wherein nucleotide interactions with the biomolecule provides electronic monitoring of DNA or genome sequencing without the use of a fluorescing element.
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