公开(公告)号：US20240182970A1

公开(公告)日：2024-06-06

申请号：US18538488

申请日：2023-12-13

申请人： SEQUENOM, INC.

发明人： Sinuhe HAHN ,  Wolfgang HOLZGREVE ,  Bernhard ZIMMERMANN ,  Ying LI

IPC分类号： C12Q1/6883 ,  C12Q1/6806

CPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q2600/156

摘要： Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≤500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of ≤500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≤500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.

公开(公告)号：US20210262035A1

公开(公告)日：2021-08-26

申请号：US17317240

申请日：2021-05-11

申请人： SEQUENOM, INC.

发明人： Sinuhe HAHN ,  Wolfgang HOLZGREVE ,  Bernhard ZIMMERMANN ,  Ying LI

IPC分类号： C12Q1/6883 ,  C12Q1/6806

摘要： Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains 500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of

公开(公告)号：US20170321279A1

公开(公告)日：2017-11-09

申请号：US15653401

申请日：2017-07-18

申请人： SEQUENOM, INC.

发明人： Sinuhe HAHN ,  Wolfgang HOLZGREVE ,  Bernhard ZIMMERMANN ,  Ying LI

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6806 ,  C12Q2600/156 ,  C12Q2565/125

摘要： Blood plasma of pregnant women contains fetal and (generally >90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≦500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of