公开(公告)号：US20230399641A1

公开(公告)日：2023-12-14

申请号：US18036399

申请日：2021-11-11

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Jia CHEN ,  Bei YANG ,  Li YANG ,  Xiaosa LI ,  Xiao WANG ,  Wenwen ZHAO ,  Lina ZHOU ,  Jifang LI

IPC: C12N15/11 ,  C12N9/22 ,  C12N9/12 ,  C12N15/90

CPC classification number: C12N15/111 ,  C12N9/22 ,  C12N9/1276 ,  C12Y207/07049 ,  C12N15/907 ,  C12N2310/20 ,  C07K2319/00

Abstract: Provided are compositions and methods for enhanced prime editing, which include a pegRNA that encodes a target mutation in a target protein, along with one or more nearby silent or conservative mutations. These silent mutations can increase the editing efficiency, without causing a change to the target protein sequence. Also provided are compositions and methods of using the improved prime editing for preventing or treating infections by SARS-CoV or SARS-CoV-2.