公开(公告)号：US20100119494A1

公开(公告)日：2010-05-13

申请号：US12689619

申请日：2010-01-19

申请人： SHILADITYA SENGUPTA ,  RAM SASISEKHARAN ,  NISHLA KEISER ,  DAVID EAVARONE ,  TANYEL KIZILTEPE BILGICER ,  AARTHI CHANDRASEKARAN ,  DAVID A. BERRY ,  KRISTINE HOLLEY

发明人： SHILADITYA SENGUPTA ,  RAM SASISEKHARAN ,  NISHLA KEISER ,  DAVID EAVARONE ,  TANYEL KIZILTEPE BILGICER ,  AARTHI CHANDRASEKARAN ,  DAVID A. BERRY ,  KRISTINE HOLLEY

IPC分类号： A61K35/12 ,  C12N5/02 ,  A61K31/715 ,  A61K38/43

CPC分类号： A61K31/726 ,  A61K31/727 ,  A61K38/47 ,  C12N5/0606 ,  C12N5/0691 ,  C12N2501/70 ,  C12N2506/02

摘要： This invention relates, in part, to methods and compositions that modulate the stem cell environment. More specifically, the invention relates, in part, to methods and compositions for modulating stem cell differentiation. Therefore, methods and compositions are provided for modulating glycosaminoglycan moieties, e.g., heparan sulfate glycosaminoglycan (HSGAG) moieties, in the microenvironment of stem cells. Methods and compositions for promoting or inhibiting embryonic stem cell differentiation (e.g., differentiation into endothelial cells) are also provided. This invention also relates, therefore, in part, to cell populations (e.g., endothelial cell populations or impoverished endothelial cell populations) that can be produced with the methods and compositions provided. Furthermore, the invention relates, in part, to tissues, and uses thereof, formed by the methods and compositions provided. Moreover, the invention also relates, in part, to methods of treatment using the methods and compositions provided.

摘要翻译： 本发明部分涉及调节干细胞环境的方法和组合物。 更具体地，本发明部分涉及用于调节干细胞分化的方法和组合物。 因此，提供了在干细胞的微环境中调节糖胺聚糖部分（例如硫酸乙酰肝素糖胺聚糖（HSGAG）部分）的方法和组合物。 还提供了用于促进或抑制胚胎干细胞分化（例如分化成内皮细胞）的方法和组合物。 因此，本发明还部分地涉及可以用所提供的方法和组合物产生的细胞群体（例如，内皮细胞群体或贫血的内皮细胞群体）。 此外，本发明部分地涉及通过所提供的方法和组合物形成的组织及其用途。 此外，本发明还部分涉及使用提供的方法和组合物的治疗方法。