公开(公告)号：US20210008196A1

公开(公告)日：2021-01-14

申请号：US16921899

申请日：2020-07-06

申请人： SICHUAN AGRICULTURAL UNIVERSITY

发明人： Anchun CHENG ,  Xingjian WEN ,  Mingshu WANG ,  Liping WU

IPC分类号： A61K39/29 ,  C12N7/00 ,  A61P31/14

摘要： Disclosed herein are a duck hepatitis A virus type 3 (DHAV-3) mutant CH-P60-117C and a construction method thereof. The DHAV-3 mutant CH-P60-117C is constructed by mutating A at position 117 of 5′-UTR of the genome of the DHAV-3 virulent strain to C; mutating T at position 1142 to A to mutate tyrosine-164 of VP0 protein of the parent strain to asparagine; and mutating C at position 4334 to A so that leucine-71 of the viral protein 2C of the parent strain is mutated to isoleucine.