公开(公告)号：US20160010085A1

公开(公告)日：2016-01-14

申请号：US14772914

申请日：2014-03-07

Applicant: SNU R&DB FOUNDATION

Inventor： Sunghoon KWON ,  Junhoi KIM ,  Taehoon RYU ,  Dongyoon OH ,  Jaekyung KOH

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12Q1/6837 ,  C12Q2565/513 ,  C12Q2565/514

Abstract: A heterologous DNA barcoding method is provided. The method includes (a) providing a DNA microarray having DNA oligonucleotide spots, which are distinguished from each other by their barcode sequences, (b) providing a microwell array having microwells whose spatial arrangement corresponds to that of the DNA spots on the DNA microarray, (c) loading a solution of samples containing target nucleic acid sequences into the microwells, (d) assembling the DNA microarray to the microwell array to form micro reaction spaces in which the DNA spots are spatially separated by the microwells, (e) allowing the oligonucleotide sequences of the DNA spots to react with the target nucleic acid sequences of the samples in the micro reaction spaces to combine the sequence information of the DNA spots with the sequence information of the samples, and (f) separating the DNA microarray and the microwell array from each other to obtain reaction products including the barcode sequences.

Abstract translation: 提供了异源DNA条形码法。 该方法包括（a）提供具有DNA寡核苷酸斑点的DNA微阵列，其通过其条形码序列彼此区分，（b）提供具有空间排列对应于DNA微阵列上的DNA斑点的微孔的微孔阵列， （c）将含有靶核酸序列的样品溶液加载到微孔中，（d）将DNA微阵列组装到微孔阵列以形成微孔反应空间，其中DNA斑点由微孔空间分开，（e）允许 DNA点的寡核苷酸序列与微反应空间中的样品的靶核酸序列反应，以将DNA斑点的序列信息与样品的序列信息组合，和（f）分离DNA微阵列和微孔 阵列相互获得包括条形码序列的反应产物。