公开(公告)号：US20230019849A1

公开(公告)日：2023-01-19

申请号：US17783040

申请日：2020-12-04

Applicant: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.

Inventor： Stephan GOTTSCHALK ,  Mireya VELASQUEZ ,  Abdullah FREIWAN ,  Abishek VAIDYA ,  Deanna LANGFITT

IPC: C12N5/0783 ,  C12N5/00 ,  B01D15/38

Abstract: Methods for preparing CD7-negative, CD3-positive T cells, which optionally express a chimeric antigen receptor, are provided as is a method of using such cells in a method for treating cancer, in particular a CD7+ cancer. In one aspect, the invention provides a method for preparing a population of CD7-negative, CD3-positive T cells by (a) performing a first selection by depleting, from a population of primary immune cells, cells that express CD7 thereby generating a population of CD7-negative cells; (b) performing a second selection by enriching, from the population of CD7-negative cells, T cells that express CD3 thereby generating a population of CD7-negative and CD3-positive T cells, and (c) incubating the population of CD7-negative and CD3-positive T cells in a culture vessel under stimulating conditions, thereby generating stimulated CD7-negative, CD3-positive T cells.