公开(公告)号：US20210369869A1

公开(公告)日：2021-12-02

申请号：US17401765

申请日：2021-08-13

Applicant: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.

Inventor： John T. GRAY

IPC: A61K48/00 ,  A61K35/76 ,  C12N15/86 ,  C12N7/00 ,  C12N15/861

Abstract: The present invention relates to nucleic acid molecules containing spacers and methods of using the same.

公开(公告)号：US20180126006A1

公开(公告)日：2018-05-10

申请号：US15573684

申请日：2016-05-13

Applicant: ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.

Inventor： John T. GRAY

IPC: A61K48/00 ,  C12N15/861 ,  A61K35/76 ,  C12N7/00

CPC classification number: A61K48/0058 ,  A61K35/76 ,  A61K48/0091 ,  C12N7/00 ,  C12N15/86 ,  C12N15/8616 ,  C12N2310/14 ,  C12N2310/17 ,  C12N2750/14143 ,  C12N2830/38

Abstract: The present invention relates to nucleic acid molecules containing spacers that can be packaged into viral particles and methods of producing them. In a first aspect, the invention features a nucleic acid molecule including a first spacer (SSI); a first inverted terminal repeat (ITR1); a cloning site (CS); a second inverted terminal repeat (ITR2); and a second spacer (SS2), such as a eukaryotic spacer; operably linked to each other in a 5′-to-3′ direction as: SS1-ITR1-CS-ITR2-SS2. In an embodiment, the invention features a vector comprising any of the above-described nucleic acid molecules. In another aspect, the invention features a plurality of viral particles including the nucleic acid molecule. The invention further includes a host cell including any of the above-described vectors.