公开(公告)号：US20190040357A1

公开(公告)日：2019-02-07

申请号：US16108707

申请日：2018-08-22

Applicant: StemCell Technologies Canada Inc.

Inventor： Andy Isamu Kokaji

IPC: C12N5/078 ,  G01N33/569 ,  A23L2/52 ,  A61K31/07 ,  A61K31/122 ,  A61K31/197 ,  A61K31/198 ,  A61K31/221 ,  A61K31/355 ,  G01N33/543 ,  G01N1/40 ,  A61K33/30 ,  A61K33/06 ,  A61K33/04 ,  A61K33/00 ,  A61K31/714 ,  A61K31/593 ,  A61K31/525 ,  A61K31/519 ,  A61K31/51 ,  A61K31/455 ,  A61K31/4415 ,  A61K31/4188 ,  A61K31/385 ,  A61K31/375 ,  A23L33/155 ,  A23L33/12 ,  A23L33/175 ,  A23L33/105 ,  A23L33/16 ,  A23L33/15 ,  A23L33/10

Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.

公开(公告)号：US11325984B2

公开(公告)日：2022-05-10

申请号：US16108707

申请日：2018-08-22

Applicant: StemCell Technologies Canada Inc.

Inventor： Andy Isamu Kokaji

IPC: G01N33/569 ,  C07K16/42 ,  C07K16/28 ,  G01N1/40

Abstract: The present invention relates to methods for separating target cells from non-target cells using immunorosettes and magnetic particles. The method involves contacting a sample containing target cells and secondary targets such as erythrocytes with an antibody composition which allows immunorosettes of the target cells and the secondary targets to form. The sample is subsequently contacted with a second antibody composition which allows the binding of magnetic particles to the formed immunorosettes and free secondary targets. The immunorosettes and secondary targets labeled with magnetic particles are separated from non-target cells using a magnetic field. The antibody composition optionally contains bifunctional antibodies or tetrameric antibody complexes.

公开(公告)号：US20210189343A1

公开(公告)日：2021-06-24

申请号：US17191830

申请日：2021-03-04

Applicant: STEMCELL Technologies Canada Inc.

Inventor： Andy Isamu Kokaji

IPC: C12N5/0783

Abstract: This disclosure provides compositions and methods for the activation and expansion of human T cells or NK cells using soluble monospecific antibody complexes.