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公开(公告)号：US09777317B2
公开(公告)日：2017-10-03
申请号：US13956677
申请日：2013-08-01
Applicant: STMicroelectronics S.r.l. , bioMérieux S.A.
Inventor： Giuseppe Emanuele Spoto , Luigi Giuseppe Occhipinti , Cristian Dall'Oglio , Crocifisso Marco Antonio Renna , Laurent Drazek
CPC classification number: C12Q1/686 , B01L3/5027 , B01L2300/0816 , B01L2300/0864
Abstract: A microfluidic device (1000-1005), comprising: a semiconductor body (2) having a first side (2a) and a second side (2b) opposite to one another, and housing, at the first side, a plurality of wells (4), having a first depth; an inlet region (30) forming an entrance point for a fluid to be supplied to the wells; a main channel (6a) fluidically connected to the inlet region, and having a second depth; and a plurality of secondary channels (6b) fluidically connecting the main channel to a respective well, and having a third depth. The first depth is higher than the second depth, which in turn is higher than the third depth. According to an aspect, the microfluidic device further comprises a cover layer (8), arranged above the first side of the semiconductor body, configured for sealing the wells and provided with at least a first valve hole (54) which extends through the cover layer and overlaps, at least partially, the secondary channels; and a flexible layer (14), arranged above the cover layer and provided with at least a protrusion (74) extending through the first valve hole towards the semiconductor body and overlapping, at least partially, the secondary channels, the flexible layer being configured such that, when a pressure is applied on it, the protrusion contacts the semiconductor body and enters the secondary channels thus fluidically isolating the wells from one another.
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2.发明申请公开(公告)号：US20170283849A1
公开(公告)日：2017-10-05
申请号：US15463855
申请日：2017-03-20
Applicant: bioMérieux S.A.
Inventor： Jean-Philippe Charrier , Bruno Colin , Laurent Drazek , Cécile Paris , Jean-Claude Raymond , Dominique Decaux
Abstract: The aim of the present invention is a method of sampling all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a culture medium (8), such as agar, for example in a Petri dish, using a probe (3) equipped with a terminal end (4), said sampling method comprising the steps of cooling the terminal end (4) of the probe (3), sticking all or part of the sample (11) of biological matter (7) to be sampled through contact, or by applying a pressure exerted by the terminal end (4) onto the sample (11) of biological matter (7), and sampling of all or part of the sample (11) of biological matter (7) so as to separate the sample (11) of biological matter (7) from the culture medium (8), a method of depositing into a container (9) or onto an analysis plate (14) all or part of a sample (11) of biological matter (7) stuck onto a frosted terminal end (4) of a probe, said depositing method including a step of separating the terminal end (4) of the probe (3) from all or part of the sample (11) of biological matter (7), as well as a device (2), a kit and an apparatus (1) implementing these methods.
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公开(公告)号：US10144948B2
公开(公告)日：2018-12-04
申请号：US15463855
申请日：2017-03-20
Applicant: bioMérieux S.A.
Inventor： Jean-Philippe Charrier , Bruno Colin , Laurent Drazek , Cécile Paris , Jean-Claude Raymond , Dominique Decaux
Abstract: The aim of the present invention is a method of sampling all or part of a sample (11) of biological matter (7), which is crude, enriched or cultured through contact with a culture medium (8), such as agar, for example in a Petri dish, using a probe (3) equipped with a terminal end (4), said sampling method comprising the steps of cooling the terminal end (4) of the probe (3), sticking all or part of the sample (11) of biological matter (7) to be sampled through contact, or by applying a pressure exerted by the terminal end (4) onto the sample (11) of biological matter (7), and sampling of all or part of the sample (11) of biological matter (7) so as to separate the sample (11) of biological matter (7) from the culture medium (8), a method of depositing into a container (9) or onto an analysis plate (14) all or part of a sample (11) of biological matter (7) stuck onto a frosted terminal end (4) of a probe, said depositing method including a step of separating the terminal end (4) of the probe (3) from all or part of the sample (11) of biological matter (7), as well as a device (2), a kit and an apparatus (1) implementing these methods.
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