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公开(公告)号：US5965725A
公开(公告)日：1999-10-12
申请号：US18170
申请日：1998-02-03
IPC分类号： C12N15/09 , A61K38/00 , A61P17/00 , A61P31/04 , C07H21/04 , C07K1/20 , C07K14/435 , C12P21/02
CPC分类号： C07K14/43509 , A61K38/00 , Y10S530/857
摘要： The present invention relates to polypeptide having a primary structure of amino acid sequence shown by Sequence List Sequence No. 1 and DNA encoding for the polypeptide. The polypeptide is obtainable by following steps (1)-(3):Step (1): extracting small granule fraction of homocytes of horseshoe crab with a buffer containing protein denaturing agent and chelating agent,Step (2): subjecting said extract to reverse phase high performance liquid chromatography,Step (3): eluting by concentration gradient elution with a hydrophobic organic solvent.Also, the polypeptide is produced by chemical synthesis. The polypeptide has similar chemical structure to defensin and is useful as gargles, disinfectants, antiseptics or antimicrobials.
摘要翻译： 本发明涉及具有序列表序列号1所示的氨基酸序列的一级结构的多肽和编码该多肽的DNA。 通过以下步骤（1） - （3）可获得多肽：步骤（1）：用含有蛋白质变性剂和螯合剂的缓冲液提取鲎的同质异构体的小颗粒级分，步骤（2）：使所述提取物逆转 阶段高效液相色谱法，步骤（3）：用疏水性有机溶剂的浓度梯度洗脱洗脱。 而且，多肽是通过化学合成产生的。 该多肽与防御素具有相似的化学结构，可用作漱口剂，消毒剂，防腐剂或抗微生物剂。
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2.发明授权Horseshoe crab hemocyte polypeptides, and preparation and DNA encoding thereof 失效马蹄蟹血细胞多肽及其制备及编码DNA
公开(公告)号：US5861378A
公开(公告)日：1999-01-19
申请号：US505617
申请日：1995-07-21
IPC分类号： C12N15/09 , A61K38/00 , A61P17/00 , A61P31/04 , C07H21/04 , C07K1/20 , C07K14/435 , C12P21/02 , A61K38/17
CPC分类号： C07K14/43509 , A61K38/00 , Y10S530/857
摘要： The present invention relates to polypeptide having a primary structure of amino acid sequence shown by Sequence List Sequence No. 1 and DNA encoding for the polypeptide. The polypeptide is obtainable by following steps (1)-(3):Step (1): extracting small granule fraction of homocytes of horseshoe crab with a buffer containing protein denaturing agent and chelating agent,Step (2): subjecting said extract to reverse phase high performance liquid chromatography,Step (3): eluting by concentration gradient elution with a hydrophobic organic solvent.Also, the polypeptide is produced by chemical synthesis. The polypeptide has similar chemical structure to defensin and is useful as gargles, disinfectants, antiseptics or antimicrobials.
摘要翻译： 本发明涉及具有序列表序列号1所示的氨基酸序列的一级结构的多肽和编码该多肽的DNA。 通过以下步骤（1） - （3）可获得多肽：步骤（1）：用含有蛋白质变性剂和螯合剂的缓冲液提取鲎的同质异体的小颗粒级分，步骤（2）：使所述提取物逆转 阶段高效液相色谱法，步骤（3）：用疏水性有机溶剂的浓度梯度洗脱洗脱。 而且，多肽是通过化学合成产生的。 该多肽与防御素具有相似的化学结构，可用作漱口剂，消毒剂，防腐剂或抗微生物剂。
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