公开(公告)号：US5972188A

公开(公告)日：1999-10-26

申请号：US675467

申请日：1996-07-03

申请人： Sallie Rice ,  Charles Browning ,  James Burke ,  Loi Nguyen

发明人： Sallie Rice ,  Charles Browning ,  James Burke ,  Loi Nguyen

IPC分类号： G01N27/447 ,  G01N27/26

CPC分类号： G01N27/44743

摘要： An apparatus and method for loading samples into a gel of an electrophoretic gel system (EGS). The preferred sample loader includes a membrane having a net negative charge, net neutral charge or no charge (preferably nitrocellulose or nylon) which releasably retains the samples such that the samples are actively released when the membrane is inserted into the gel of an EGS. In one preferred embodiment, the sample loader includes a substrate having a plurality of sample loading areas extending therefrom. In an alternative embodiment, the membrane is substantially thick and serves as its own substrate. In another embodiment, sample inhibiting agents such as hydrophobic ink are formed through the membrane to inhibit the diffusion of samples between sample loading areas. Each sample loading area includes an affixed membrane. In use, one or more samples to be subjected to electrophoretic action are applied to the membrane before the membrane is inserted into a previously polymerized gel. The membrane advantageously prevents contamination of electrode buffer solution and cross-talk or mixing between samples by inhibiting the diffusion of sample material into the gel or buffer solution before the gel is activated by a release mechanism. Once the gel is activated by the release mechanism, the membrane releases substantially all of the samples into the gel where the samples are subjected to the electrophoretic separation and identification process.

摘要翻译： 将样品加载到电泳凝胶系统（EGS）的凝胶中的装置和方法。 优选的样品装载器包括具有净负电荷，净中性电荷或无电荷（优选硝酸纤维素或尼龙）的膜，其可释放地保留样品，使得当膜插入EGS的凝胶中时样品被主动释放。 在一个优选实施例中，样品装载器包括具有从其延伸的多个样品加载区域的基底。 在替代实施例中，膜基本上是厚的并且用作其自身的基底。 在另一个实施方案中，通过膜形成样品抑制剂如疏水性油墨，以抑制样品在样品加载区域之间的扩散。 每个样品装载区域都包括固定膜。 在使用中，将一个或多个要进行电泳作用的样品施加到膜上，然后将膜插入到先前聚合的凝胶中。 该膜有利地防止电极缓冲溶液的污染和样品之间的串扰或混合，通过在凝胶被释放机制激活之前通过抑制样品材料扩散到凝胶或缓冲溶液中。 一旦凝胶被释放机制激活，膜将基本上将所有样品释放到凝胶中，其中样品进行电泳分离和鉴定过程。