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1.发明授权
公开(公告)号：US4855930A
公开(公告)日：1989-08-08
申请号：US31288
申请日：1987-03-27
CPC分类号： G01N33/5302 , G01N21/6408 , G01N21/6428 , G01N2201/0697
摘要： A method and apparatus for time-resolved fluorescence spectroscopy is described in which laser light from a single pulse is used to excite fluorescent photons in a sample, which fluorescence is detected by a PMT optimized for linearity and response time to produce photoelectrons which generate a current at the PMT anode. This current is discharged through an R/C network to produce a voltage amplitude waveform which is converted to an optical image, intensified, stored and digitized. The digitized version of the optical image is processed in a data processor to calculate the true fluorescence impulse response.
摘要翻译： 描述了用于时间分辨荧光光谱的方法和装置，其中使用来自单个脉冲的激光来激发样品中的荧光光子，该荧光由针对线性和响应时间优化的PMT检测以产生产生电流的光电子 在PMT阳极。 该电流通过R / C网络放电，产生电压幅度波形，转换为光学图像，增强，存储和数字化。 数字化版本的光学图像在数据处理器中进行处理，以计算真实的荧光脉冲响应。
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公开(公告)号：US4923819A
公开(公告)日：1990-05-08
申请号：US31408
申请日：1987-03-27
CPC分类号： G01N21/6408 , G01N33/5302 , G01N21/6428 , G01N2201/0697 , Y10S435/973 , Y10S436/805 , Y10S436/815 , Y10S436/818
摘要： Fluorescence immunoassay for determining single or multiple analytes based upon a single measurement of fluorescence are described.
摘要翻译： 描述了基于单次测量荧光测定单个或多个分析物的荧光免疫测定。
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公开(公告)号：US06485981B1
公开(公告)日：2002-11-26
申请号：US09362517
申请日：1999-07-28
IPC分类号： G01N3392
CPC分类号： G01N33/92
摘要： The invention relates to a method and apparatus for imaging and documenting fingerprints. A fluorescent dye brought in intimate proximity with the lipid residues of a latent fingerprint is caused to fluoresce on exposure to light energy. The resulting fluorescing image may be recorded photographically.
摘要翻译： 本发明涉及一种用于成像和记录指纹的方法和装置。 导致与潜在指纹的脂质残留紧密接近的荧光染料在暴露于光能时发荧光。 所得到的荧光图像可以照相地记录。
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公开(公告)号：US5818582A
公开(公告)日：1998-10-06
申请号：US818881
申请日：1997-03-17
CPC分类号： G01J3/4406
摘要： A phase fluorometer employs a baseband frequency generator to generate a sample baseband signal at a first frequency f.sub.o and a reference signal correlated to the sample baseband signal. A second signal generator generates a carrier signal having a second frequency f. The sample baseband signal and the carrier signal are combined to form an up-converted sample signal having a frequency f.+-.f.sub.o. A light source is modulated at the up-converted sample signal frequency f.+-.f.sub.o to illuminate a sample. A photo detector detects fluorescence emissions from a sample excited by the excitation light and converts the emission into a fluorescence signal. A down-converter combines the carrier signal and fluorescence signal to form a phase-shifted and down-converted sample signal. A processor digitally processes the phase-shifted and demodulated sample signal and the reference signal to determine the fluorescence lifetime.
摘要翻译： 相位荧光计采用基带频率发生器产生第一频率fo的采样基带信号和与采样基带信号相关的参考信号。 第二信号发生器产生具有第二频率f的载波信号。 样本基带信号和载波信号被组合以形成具有频率f +/- fo的上变频采样信号。 在上转换的采样信号频率f +/- fo处调制光源以照射样品。 光检测器检测由激发光激发的样品的荧光发射，并将发射转换成荧光信号。 下转换器组合载波信号和荧光信号以形成相移和下变换的采样信号。 处理器对相移和解调的采样信号和参考信号进行数字处理，以确定荧光寿命。
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