公开(公告)号：US20140057322A1

公开(公告)日：2014-02-27

申请号：US13856301

申请日：2013-04-03

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Ko-bong CHOI ,  Joo-won RHEE ,  Sea-hee KIM

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2525/121 ,  C12Q2525/191 ,  C12Q2531/125

Abstract: A method of amplifying RNA in a sample and a method of amplifying a pool of RNA is provided. Gene loss and amplification bias may be reduced using the methods. Also, directionality may be preserved in the amplified RNA. The methods may be applied in sequence analysis as well as in general molecular diagnostic areas.

Abstract translation: 提供了扩增样品中的RNA的方法和扩增RNA库的方法。 使用这些方法可以减少基因损失和放大偏差。 此外，可以在扩增的RNA中保留方向性。 这些方法可以应用于序列分析以及一般分子诊断领域。

公开(公告)号：US20140147840A1

公开(公告)日：2014-05-29

申请号：US13904769

申请日：2013-05-29

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Sea-hee KIM ,  Joo-won RHEE ,  Ko-bong CHOI

IPC: G01N21/64

CPC classification number: C12Q1/6844 ,  C12Q2521/131 ,  C12Q2525/173 ,  C12Q2533/101 ,  C12Q2563/107

Abstract: Methods of labeling and detecting a target nucleic acid by incubating a target nucleic acid with a terminal transferase to extend a terminus of the target nucleic acid and provide an extended region; hybridizing the extended region of the target nucleic acid with a template polynucleotide having a nucleotide sequence complementary to the extended region to obtain a hybridization product; and incubating the hybridization product with a nucleic acid polymerase and either a deoxynucleotide triphosphate (dNTP) having a detectable label or nucleotide triphosphate (NTP) having a detectable label to further extend the extended target nucleic acid.

Abstract translation: 通过将靶核酸与末端转移酶孵育以延伸靶核酸的末端并提供延伸区域来标记和检测靶核酸的方法; 将靶核酸的延伸区域与具有与延伸区域互补的核苷酸序列的模板多核苷酸杂交以获得杂交产物; 并用杂交产物与核酸聚合酶和具有可检测标记或具有可检测标记的核苷酸三磷酸（NTP）的脱氧核苷酸三磷酸（dNTP）孵育，以进一步扩展延伸的靶核酸。

公开(公告)号：US20130157259A1

公开(公告)日：2013-06-20

申请号：US13715955

申请日：2012-12-14

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Ko-bong CHOI ,  Joo-won RHEE ,  Sea-hee KIM

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12P19/34 ,  C12Q1/6851 ,  C12Q2521/501 ,  C12Q2527/101 ,  C12Q2531/125 ,  C12Q2563/159

Abstract: Provided are methods of efficiently amplifying DNA from RNA in sample, methods of efficiently estimating an amount of RNA in a sample, and compositions for efficiently amplifying DNA from RNA in a sample.

Abstract translation: 提供了有效地扩增样品中RNA的DNA的方法，有效估计样品中RNA量的方法，以及用于从样品中有效地扩增DNA的组合物。

公开(公告)号：US20140162276A1

公开(公告)日：2014-06-12

申请号：US14082250

申请日：2013-11-18

Applicant: Samsung Electronics Co., Ltd.

Inventor： Joo-won RHEE ,  Sea-hee KIM ,  Ko-bong CHOI

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q2525/307 ,  C12Q2531/119

Abstract: Provided are methods of efficiently amplifying or analyzing a target nucleic acid and a kit and a composition to efficiently amplify a target nucleic acid.

Abstract translation: 提供有效扩增或分析靶核酸和试剂盒的方法以及有效扩增靶核酸的组合物。

公开(公告)号：US20130157269A1

公开(公告)日：2013-06-20

申请号：US13715928

申请日：2012-12-14

Applicant: SAMSUNG ELECTRONICS CO., LTD.

Inventor： Sea-hee KIM ,  Joo-won RHEE ,  Ko-bong CHOI

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q2521/501 ,  C12Q2527/101 ,  C12Q2531/125 ,  C12Q2563/159

Abstract: A method of amplifying DNA from RNA in a sample by using circular RNA is provided.

Abstract translation: 提供了通过使用环状RNA从样品中的RNA扩增DNA的方法。