公开(公告)号：US20190080760A1

公开(公告)日：2019-03-14

申请号：US15690189

申请日：2017-08-29

Applicant: Dodo OmniData, Inc.

Inventor： Paul F. Predki ,  Maja Cassidy

IPC: G11C13/02 ,  G06N3/12 ,  G06F19/22 ,  C12Q1/6869

CPC classification number: G11C13/02 ,  B82Y10/00 ,  C12Q1/68 ,  C12Q1/6869 ,  G06N3/123 ,  G16B30/00 ,  C12Q2563/116 ,  C12Q2565/631 ,  C12Q2521/131

Abstract: The disclosure provides a novel system of storing information using a charged polymer, e.g., DNA, the monomers of which correspond to a machine-readable code, e.g., a binary code, and which can be synthesized and/or read using a novel nanochip device comprising nanopores; novel methods and devices for synthesizing oligonucleotides in a nanochip format; novel methods for synthesizing DNA in the 3′ to 5′ direction using topoisomerase; novel methods and devices for reading the sequence of a charged polymer, e.g., DNA, by measuring capacitive variance as the polymer passes through the nanopore; and further provides compounds, compositions, methods and devices useful therein.

公开(公告)号：US20180223321A1

公开(公告)日：2018-08-09

申请号：US15898460

申请日：2018-02-17

Applicant: SWIFT BIOSCIENCES, INC.

Inventor： VLADIMIR MAKAROV ,  LAURIE KURIHARA

IPC: C12P19/34 ,  C12Q1/6855 ,  C12N9/12 ,  C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2521/131 ,  C12Q2525/186 ,  C12Q2525/204 ,  C12Q2537/163

Abstract: The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate poly—nucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3′ end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing.

公开(公告)号：US09896709B2

公开(公告)日：2018-02-20

申请号：US14384113

申请日：2013-03-13

Applicant: SWIFT BIOSCIENCES, INC.

Inventor： Vladimir Makarov ,  Laurie Kurihara

IPC: C12Q1/68 ,  C12N9/12 ,  C12P19/34

CPC classification number: C12P19/34 ,  C12N9/1241 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q2521/131 ,  C12Q2525/186 ,  C12Q2525/204 ,  C12Q2537/163

Abstract: The present invention is directed to methods and compositions for adding tails of specific lengths to a substrate polynucleotide. The invention also contemplates methods and compositions for immobilization of tailed substrates to a solid support. The disclosure contemplates that the attenuator molecule is any biomolecule that associates with a tail sequence added to a substrate polynucleotide and controls the addition of a tail sequence to the 3′ end of the substrate polynucleotide. The sequence that is added to the substrate polynucleotide is referred to herein as a tail sequence, or simply a tail, and the process of adding a nucleotide to a substrate polynucleotide is referred to herein as tailing.

公开(公告)号：US09822394B2

公开(公告)日：2017-11-21

申请号：US15054227

申请日：2016-02-26

Applicant: Cambridge Epigenetix Limited

Inventor： Tobias William Barr Ost ,  Neil Matthew Bell

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/131 ,  C12Q2523/107 ,  C12Q2523/125 ,  C12Q2525/121 ,  C12Q2525/301 ,  C12Q2535/122 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2543/101 ,  C12Q2565/1015

Abstract: This invention relates to the preparation of nucleic acid samples for analysis. The invention may be particularly useful for single stranded samples. Embodiments of the invention involve the attachment of double stranded or hairpin oligonucleotides using template independent polymerase enzymes in the preparation of nucleic acid sequencing libraries.

公开(公告)号：US09394564B2

公开(公告)日：2016-07-19

申请号：US12095485

申请日：2006-11-29

Applicant: Alexander Seitz

Inventor： Alexander Seitz

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2539/113 ,  C12Q2521/131 ,  C12Q2525/179 ,  C12Q2521/107

Abstract: The present invention provides a method for amplifying a pool of polynucleotide molecules in a sample, characterized by the steps of a) obtaining a sample or RNA and reverse transcription of entire RNA molecules thus creating full length cDNA or obtaining a sample of full length cDNA, b) tailing the 3′ end of the transcribed cDNA with a polynucleotide tail after the 3′ end, c) amplification of the cDNA using a pair of primers, wherein a first 3′ primer is specific for the 5′ end of the cDNA and a second 5′ primer is specific for the a upstream portion of the polynucleotide tail and the next 1 to 10 nucleotides upstream of the 3′polynucleotide tail of the cDNA.

Abstract translation: 本发明提供了用于扩增样品中多核苷酸分子池的方法，其特征在于以下步骤：a）获得样品或RNA并逆转整个RNA分子，从而产生全长cDNA或获得全长cDNA样品， b）在3'末端之后用多核苷酸尾部拖尾转录的cDNA的3'末端，c）使用一对引物扩增cDNA，其中第一个3'引物对cDNA的5'末端是特异性的，以及 第二个5'引物对于多核苷酸尾部的上游部分和cDNA的3'多核苷酸尾部上游的1到10个核苷酸是特异性的。

公开(公告)号：US20150322510A1

公开(公告)日：2015-11-12

申请号：US14431257

申请日：2013-09-25

Applicant: CEPHEID

Inventor： Russell HIGUCHI ,  Edwin Wei-Lung LAI ,  Sergey LOKHOV

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12N15/111 ,  C12N2310/141 ,  C12N2320/10 ,  C12Q1/6853 ,  C12Q2600/16 ,  C12Q2600/178 ,  C12Q2525/207 ,  C12Q2537/143 ,  C12Q2521/131 ,  C12Q2525/173 ,  C12Q2525/301 ,  C12Q2565/1015

Abstract: The present invention provides method for amplifying a specific RNA molecule in a sample, the method comprising: (a) adding a poly(ribonucleotide) sequence to RNA molecules in the sample; (b) reverse transcribing the poly-adenylated RNA molecules using a reverse primer comprising a sequence that anneals to said poly(ribonucleotide) sequence; and (c) amplifying and detecting the cDNA molecule(s) using the same reverse primer and using a forward primer specific for the RNA molecule to be detected; wherein at least one of the forward and reverse primers comprises a hairpin primer. The invention also provides kits useful for practicing this method.

Abstract translation: 本发明提供扩增样品中特异性RNA分子的方法，所述方法包括：（a）向样品中的RNA分子加入聚（核糖核苷酸）序列; （b）使用包含与所述多核苷酸核苷酸序列退火的序列的反向引物逆转录多聚腺苷酸化的RNA分子; 和（c）使用相同的反向引物扩增和检测cDNA分子，并使用对待检测的RNA分子特异性的正向引物; 其中所述正向引物和反向引物中的至少一个包含发夹引物。 本发明还提供了用于实施该方法的试剂盒。

公开(公告)号：US20130143771A1

公开(公告)日：2013-06-06

申请号：US13733302

申请日：2013-01-03

Applicant: DUKE UNIVERSITY

Inventor： Ashutosh Chilkoti

IPC: C12Q1/68 ,  G01N33/53

CPC classification number: C12Q1/682 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B50/18 ,  G01N33/5302 ,  G01N33/54353 ,  G01N33/54393 ,  C12Q2525/313 ,  C12Q2521/131

Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.

Abstract translation: 描述了诸如其上具有非结垢表面的生物传感器的物品。 该制品包括：（a）具有表面部分的基底; （b）表面部分上的连接层; （c）包含在所述连接层上形成的刷分子的聚合物层; 和（d）任选但优选地，偶联到刷分子的特异性结合对（例如蛋白质，肽，抗体，核酸等）的第一成员。 聚合物层优选通过其上单体单元的表面引发聚合（SIP）的方法形成。 优选地，每个单体单元包含具有至少一个与其结合的蛋白质抗性头基团的单体（例如，乙烯基单体）核心基团，从而在表面部分上形成刷分子。 还描述了使用制品的方法。

公开(公告)号：US20120202198A1

公开(公告)日：2012-08-09

申请号：US12377457

申请日：2007-08-13

Applicant: Holger Engel ,  Subrahmanyam Yerramilli ,  Martin Kreutz ,  Dirk Loeffert ,  Christian Korfhage

Inventor： Holger Engel ,  Subrahmanyam Yerramilli ,  Martin Kreutz ,  Dirk Loeffert ,  Christian Korfhage

IPC: C12P19/34 ,  C12N9/12 ,  C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2527/101 ,  C12Q2521/131 ,  C12Q2521/107

Abstract: This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture.

Abstract translation: 本发明涉及在酶反应中合成样品中cDNA的方法，其中该方法包括以下步骤：同时制备具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，缓冲液，至少 一个核糖核苷酸，至少一个脱氧核糖核苷酸，锚定寡核苷酸; 加入包含核糖核酸的样品; 以及先前步骤的试剂在一个或多个温度步骤中的温育，其被选择为使得第一酶和第二酶显示活性。 本发明还涉及包含具有多聚腺苷酸化活性的第一种酶，具有逆转录酶活性的第二种酶，任选的缓冲液，任选的至少一种核糖核苷酸，任选的至少一种脱氧核糖核苷酸和任选的锚定寡核苷酸的反应混合物。 此外，本发明涉及包含相应反应混合物的试剂盒。

公开(公告)号：US20110250648A1

公开(公告)日：2011-10-13

申请号：US13081272

申请日：2011-04-06

Applicant: Sherilynn Manalili Wheeler ,  James C. Hannis ,  Steven A, Hofstadler

Inventor： Sherilynn Manalili Wheeler ,  James C. Hannis ,  Steven A, Hofstadler

IPC: C12P19/34 ,  C12N9/12

CPC classification number: C12P19/34 ,  C07K14/4703 ,  C07K14/5406 ,  C12Q1/686 ,  C12Q2521/131 ,  C12Q2525/161 ,  C12Q2527/125 ,  C12Q2565/627

Abstract: The present invention relates to systems and methods for amplifying nucleic acid. In particular, systems and methods are provided for inhibiting polymerase based terminal transferase activity within a polynucleotide amplification setting (e.g., polymerase chain reaction). In addition, systems and methods are provided for generating amplified products generated with polynucleotide amplification techniques having reduced 3′ non-templated nucleotide addition.

Abstract translation: 本发明涉及用于扩增核酸的系统和方法。 特别地，提供了用于在多核苷酸扩增设置（例如，聚合酶链式反应）内抑制基于聚合酶的末端转移酶活性的系统和方法。 此外，提供了用于产生用具有减少的3'非模板核苷酸添加的多核苷酸扩增技术产生的扩增产物的系统和方法。

公开(公告)号：US07713689B2

公开(公告)日：2010-05-11

申请号：US11521651

申请日：2006-09-15

Applicant: Ashutosh Chilkoti

Inventor： Ashutosh Chilkoti

IPC: C12Q1/00 ,  C12Q1/70

CPC classification number: C12Q1/682 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B50/18 ,  G01N33/5302 ,  G01N33/54353 ,  G01N33/54393 ,  C12Q2525/313 ,  C12Q2521/131

Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.

Abstract translation: 描述了诸如其上具有非结垢表面的生物传感器的物品。 该制品包括：（a）具有表面部分的基底; （b）表面部分上的连接层; （c）包含在所述连接层上形成的刷分子的聚合物层; 和（d）任选但优选地，偶联到刷分子的特异性结合对（例如蛋白质，肽，抗体，核酸等）的第一成员。 聚合物层优选通过其上单体单元的表面引发聚合（SIP）的方法形成。 优选地，每个单体单元包含具有至少一个与其结合的蛋白质抗性头基团的单体（例如，乙烯基单体）核心基团，从而在表面部分上形成刷分子。 还描述了使用制品的方法。