公开(公告)号：US20070196894A1

公开(公告)日：2007-08-23

申请号：US11677302

申请日：2007-02-21

申请人： Sang-jun SIM ,  Chang-deok KANG

发明人： Sang-jun SIM ,  Chang-deok KANG

IPC分类号： C12P23/00 ,  C07C35/18

CPC分类号： C07C403/24 ,  C12P23/00

摘要： There is provided a method of separating free-astaxanthin selectively from green algae and, more particularly, to a method of separating free-astaxanthin selectively from Haematococcus pluvialis, the method comprising: mixing a cell culture containing Haematococcus pluvialis with an alkanic solvent and stirring, thereby obtaining an alkanic solvent extract containing astaxanthin material including free-astaxanthin and astaxanthin ester (step 1); and mixing the alkanic solvent extract with an alcohol and stirring, thereby obtaining an alcoholic extract containing free-astaxanthin (step 2). The present invention can not only maximize the efficiency of separating astaxanthin in high concentration by providing a very simple process technique capable of recovering free-astaxanthin selectively from H. pluvialis culture with high efficiency, but also realize the economical and simple operation of the process of separating free-astaxanthin by repeatedly using an alkanic solvent as a mediating solvent for extracting free-astaxanthin. Such a separation method can be used in extracting and separating a carotinoid analogue containing astaxanthin from a useful microalgae culture in addition to Haematococcus species.

摘要翻译： 提供了一种从绿藻中选择性分离游离虾青素的方法，更具体地说，涉及一种从红潮球菌中选择性分离游离虾青素的方法，该方法包括：将含有积雪球菌的细菌培养物与烷烃溶剂混合并搅拌， 从而得到含有游离虾青素和虾青素的虾青素的烷烃溶剂提取物（步骤1）。 并将该烷醇溶剂提取物与醇混合并搅拌，从而得到含有游离虾青素的醇提取物（步骤2）。 本发明不仅可以通过提供一种非常简单的工艺技术来最大限度地提高高浓度虾青素的分离效率，而且能够高效率地从胡萝卜水溶液中选择性地回收游离虾青素，同时也实现了经济，简便的操作过程 通过反复使用烷烃溶剂作为提取游离虾青素的介质溶剂分离游离虾青素。 这种分离方法可以用于从除血球菌属之外的有用的微藻培养物中提取和分离含有虾青素的类胡萝卜素类似物。

公开(公告)号：US20070166703A1

公开(公告)日：2007-07-19

申请号：US11617064

申请日：2006-12-28

申请人： Sang-jun SIM ,  Chang-deok KANG

发明人： Sang-jun SIM ,  Chang-deok KANG

IPC分类号： C12Q1/70 ,  G01N33/569 ,  G01N33/554 ,  C12M3/00

CPC分类号： G01N33/569 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54373 ,  G01N33/554 ,  Y10S436/805

摘要： There is provided a method of detecting a pathogenic microorganism in real-time, using a modified flow-type surface plasmon resonance (SPR) biosensor, comprising the steps of: i) performing, in a batch-type, an immune reaction of a pathogenic microorganism and an antibody thereto; ii) selectively separating the pathogenic microorganism bound with the antibody; and iii) binding the pathogenic microorganism bound with the antibody on a surface of a chip of a flow-type SPR sensor system in real-time.

摘要翻译： 提供了一种使用修饰的流式表面等离子体共振（SPR）生物传感器实时检测病原微生物的方法，包括以下步骤：i）以间歇式的方式进行病原体的免疫反应 微生物及其抗体; ii）选择性分离与抗体结合的致病微生物; 和iii）将与抗体结合的病原微生物实时结合在流式SPR传感器系统的芯片的表面上。