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    Separation of polar lipids from krill oil extract
    2.
    发明授权
    Separation of polar lipids from krill oil extract 有权

    公开(公告)号：US09650590B2

    公开(公告)日：2017-05-16

    申请号：US15382085

    申请日：2016-12-16

    申请人： Orochem Technologies, Inc.

    发明人： Anil R. Oroskar ,  Asha A. Oroskar ,  Alexander B. Smetana ,  Slobodan Milasinovic ,  Xuejun Zang

    IPC分类号： C07C403/24 ,  C11B3/10 ,  C11B3/00

    CPC分类号： C07C403/24 ,  C07C2601/10 ,  C07C2601/16 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/10

    摘要： Disclosed is a chromatographic process complex for the refining of krill oil extract including desalting, removal of impurities such as trimethylamine oxide (TMAO), and the production of krill oil products including desalted krill oil extract, polar lipid products having polar lipid contents greater than 50 wt-% on a dry or solvent free basis, neutral lipid streams for biodiesel production and astaxanthin. The refinery includes a continuous desalting zone, a fixed bed polar lipid extraction zone to adsorb neutral lipids and astaxanthin to provide a polar lipid extract stream comprising solvent and polar lipids and being essentially free of neutral lipids and astaxanthin, and an astaxanthin separation zone to recover essentially pure astaxanthin and provide a neutral lipid stream. The enriched products of the krill oil refinery are essentially free of TMAO and salt and provide products which can be used as dietary supplements and as medicinal additives.

    KRILL OIL REFINERY FOR PURIFICATION OF KRILL OIL EXTRACT
    4.
    发明申请
    KRILL OIL REFINERY FOR PURIFICATION OF KRILL OIL EXTRACT 有权
    KRILL油精炼用于提炼KRILL油提取物

    公开(公告)号：US20160229781A1

    公开(公告)日：2016-08-11

    申请号：US14619102

    申请日：2015-02-11

    申请人： Orochem Technologies, Inc.

    发明人： Anil R. OROSKAR ,  Asha A. Oroskar ,  Alexander B. Smetana ,  Slobodan Milasinovic ,  Xuejun Zang

    IPC分类号： C07C45/85

    CPC分类号： C07C403/24 ,  C07C2601/10 ,  C07C2601/16 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/10

    摘要： Disclosed is a chromatographic process complex for the refining of krill oil extract including desalting, removal of impurities such as trimethylamine oxide (TMAO), and the production of krill oil products including desalted krill oil extract, polar lipid products having polar lipid contents greater than 50 wt-% on a dry or solvent free basis, neutral lipid streams for biodiesel production and astaxanthin. The refinery includes a continuous desalting zone, a fixed bed polar lipid extraction zone to adsorb neutral lipids and astaxanthin to provide a polar lipid extract stream comprising solvent and polar lipids and being essentially free of neutral lipids and astaxanthin, and an astaxanthin separation zone to recover essentially pure astaxanthin and provide a neutral lipid stream. The enriched products of the krill oil refinery are essentially free of TMAO and salt and provide products which can be used as dietary supplements and as medicinal additives.

    摘要翻译： 公开了一种用于精制磷虾油提取物的色谱法复合物，包括脱盐，去除杂质如三甲胺氧化物（TMAO），以及产生包括脱盐磷虾油提取物的磷虾油产品，极性脂质含量大于50 以干或无溶剂为基准，用于生物柴油生产的中性脂质流和虾青素。 炼油厂包括连续脱盐区，固定床极性脂质提取区，用于吸附中性脂质和虾青素，以提供极性脂质提取物流，其包含溶剂和极性脂质，并且基本上不含中性脂质和虾青素，以及虾青素分离区以回收 基本上是纯的虾青素并提供中性脂质流。 磷虾炼油厂的富集产品基本上不含TMAO和盐，并提供可用作膳食补充剂和药用添加剂的产品。

    METHODS FOR PRODUCING CAROTENOIDS FROM FERMENTATION BY-PRODUCTS
    6.
    发明申请
    METHODS FOR PRODUCING CAROTENOIDS FROM FERMENTATION BY-PRODUCTS 审中-公开
    从发酵副产品生产碳酸的方法

    公开(公告)号：US20160083766A1

    公开(公告)日：2016-03-24

    申请号：US14891004

    申请日：2014-05-06

    申请人： DIREVO Industrial Biotechnology GmbH

    发明人： Joachim Englert ,  Klaudija Milos

    IPC分类号： C12P23/00 ,  A23K1/16 ,  C07C403/24

    CPC分类号： C12P23/00 ,  A23K20/179 ,  A23K20/28 ,  C07C403/24 ,  C07C2601/16 ,  C09B61/00 ,  C11B3/10 ,  C11B13/00 ,  C12P5/00 ,  Y02W30/74

    摘要： The present technology relates to methods for extracting carotenoids like β-carotene or lutein from oil obtained from/as a by-product derived from a feedstock material like starch-containing material in a processes for producing fermentation products by-products derived from a fermentative production process, in particular from an ethanol fermentation process, wherein the by-product is selected from the group consisting of distillers' wet grain (DWG), distillers' dried grains (DDG), distillers' solubles (DS), distillers' dried solubles (DDS), distillers' dried grain with solubles (DDGS), and mixtures thereof.

    摘要翻译： 本技术涉及在从发酵产生的发酵产物副产物的制备方法中从作为衍生自原料的原料如含淀粉的材料获得的油中提取类胡萝卜素，如胡萝卜素或叶黄素的方法。 生产过程，特别是乙醇发酵过程，其中副产物选自蒸馏器的湿颗粒（DWG），蒸馏器干燥谷物（DDG），酒糟可溶物（DS），蒸馏器干燥的可溶物 （DDS），具有可溶物的酒糟干粒（DDGS）及其混合物。

    Production of isoprenoids
    8.
    发明授权
    Production of isoprenoids 有权
    生产类异戊二烯

    公开(公告)号：US09102954B2

    公开(公告)日：2015-08-11

    申请号：US13865072

    申请日：2013-04-17

    申请人： Arkion Life Sciences, LLC

    发明人： James R. Millis ,  Julie Maurina-Brunker ,  Thomas W. McMullin

    IPC分类号： C12N1/14 ,  C12N15/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C07H21/04 ,  C12P7/04 ,  C12N15/81 ,  C07C403/08 ,  C07C403/24 ,  C07D311/72 ,  C12N9/02 ,  C12N9/10 ,  C12P9/00 ,  C12P17/06 ,  C12P23/00 ,  H01Q1/00

    CPC分类号： C12P7/04 ,  C07C403/08 ,  C07C403/24 ,  C07C2601/16 ,  C07D311/72 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1085 ,  C12N15/81 ,  C12P9/00 ,  C12P17/06 ,  C12P23/00 ,  C12Y101/01034 ,  H01Q1/00

    摘要： A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid.

    摘要翻译： 提供了制备类异戊二烯化合物的重组酵母。 酵母包含内源性甲羟戊酸途径，其包含（i）将乙酰辅酶A转化为乙酰乙酰辅酶A的酶，（ii）将乙酰乙酰辅酶A转化为羟甲基戊二酰辅酶A的酶，（iii）将羟甲基戊二酰辅酶A转化成甲羟戊酸 酸，（iv）将甲羟戊酸转化为磷酸戊糖酸的酶，（v）将磷酸戊糖醛酸转化为磷酸戊糖醛酸的酶，以及（vi）将磷酸戊糖转化为异戊烯焦磷酸的酶。 酵母进一步包含编码将羟甲基戊二酰辅酶A转化为甲羟戊酸的酶的异源核酸序列，将甲羟戊酸转化为磷酸戊糖酸的酶，将磷酸戊糖醛酸转化为磷酸甲羟戊酸的酶，以及将磷酸戊糖转化为异戊烯基焦磷酸的酶。 与不含编码将羟甲基戊二酰辅酶A转化为甲羟戊酸的酶的异源核酸分子的酵母相比，重组酵母中的碳流量增加。

    Process for preparing xanthophyll crystal
    9.
    发明授权
    Process for preparing xanthophyll crystal 有权
    制备叶黄素晶体的方法

    公开(公告)号：US08921615B2

    公开(公告)日：2014-12-30

    申请号：US13825772

    申请日：2011-12-31

    申请人： Qingguo Lu ,  Yunhe Lian ,  Xuehui Su ,  Xiaodong An ,  Yuanxin Cheng ,  Lijun Qi

    发明人： Qingguo Lu ,  Yunhe Lian ,  Xuehui Su ,  Xiaodong An ,  Yuanxin Cheng ,  Lijun Qi

    IPC分类号： C07C35/21

    CPC分类号： C07C35/21 ,  C07C403/24 ,  C07C2601/16

    摘要： Disclosed a process for preparing a xanthophyll crystal, comprising: dissolving the plant extract containing a xanthophyll ester in n-hexane, then filtering the mixture; adding acetone to the filtrate, filtering and collecting a filter cake; mixing the filter cake with soybean oil and ethanol uniformly; saponifying the mixed solution with alkaline aqueous solution; then adding an acidic solution thereto until the mixed solution becomes acidic, concentrating under reduced pressure to obtain a pasty substance; adding n-hexane to the pasty saponified product, standing still and then conducting a solid-liquid separation; washing the resulting solid substance with deionized water; adding a mixed solvent to the washed solid substance, dissolving it with stirring; and then adding n-hexane thereto and standing still to recrystallize. According to the application, organic solvents are used to treat the plant extract and remove non-xanthophyll ester compounds in order to improve the efficiency of the saponification reaction; the saponified solution is concentrated under acidic condition at reduced pressure, then extracted with an organic solvent for saving water; purifying a xanthophyll crystal with a mixed solvent in order to significantly increase the purity of a xanthophyll crystal and proportion of trans-xanthophyll.

    摘要翻译： 披露了一种制备叶黄素晶体的方法，包括：将含有叶黄素酯的植物提取物溶解在正己烷中，然后过滤混合物; 向滤液中加入丙酮，过滤收集滤饼; 将滤饼与大豆油和乙醇均匀混合; 用碱性水溶液皂化混合溶液; 然后加入酸性溶液直到混合溶液变成酸性，减压浓缩得到糊状物质; 向糊状皂化产物中加入正己烷，静置，然后进行固液分离; 用去离子水洗涤所得固体物质; 向混合溶剂中加入混合溶剂，搅拌溶解; 然后加入正己烷，静置，重结晶。 根据应用，有机溶剂用于处理植物提取物并除去非叶黄素酯化合物，以提高皂化反应的效率; 皂化溶液在酸性条件下减压浓缩，然后用有机溶剂萃取以节约用水; 用混合溶剂净化叶黄素晶体，以显着提高叶黄素晶体的纯度和反式叶黄素的比例。