公开(公告)号：US20070281331A1

公开(公告)日：2007-12-06

申请号：US11624380

申请日：2007-01-18

申请人： Sangmo KOO ,  Songwan JIN ,  Yongku LEE ,  Jung Yul YOO ,  Jae-young SUNG ,  Chang-Soo LEE

发明人： Sangmo KOO ,  Songwan JIN ,  Yongku LEE ,  Jung Yul YOO ,  Jae-young SUNG ,  Chang-Soo LEE

IPC分类号： C12Q1/04 ,  G06T7/20

CPC分类号： G06T7/20 ,  G06K9/00127 ,  G06T2207/30024 ,  G06T2207/30241

摘要： Provided is a method of tracking a swimming path of a bacterium which can exactly track the swimming path of the bacterium by modeling the bacterium as ellipsoidal in shape based on an image of the bacterium obtained while the ellipsoidal bacterium swims near a solid surface. The method of tracking a swimming path of a bacterium which is formed in an ellipsoidal shape and swims in a swimming space formed between a solid surface and an imaginary surface parallel to the solid surface includes the steps of: transfecting a fluorescent gene into the bacterium; arranging the bacterium in the swimming space to swim; totally reflecting light irradiated to the solid surface, and forming an evanescent field in the swimming space; taking a picture of the bacterium expressing the fluorescent gene, which emits light in the evanescent field, at respective moments while the bacterium swims, and obtaining an image of the bacterium expressing the fluorescent gene at the respective moments; and fitting the image of the bacterium obtained at the respective moments to an ellipsoidal shape, and setting the shape of the bacterium as the ellipsoidal shape and a position of the bacterium with respect to the solid surface.

摘要翻译： 提供了一种跟踪细菌的游泳路径的方法，其可以基于当椭球形细菌在固体表面附近游动时获得的细菌的图像来模拟细菌的椭圆形形状，从而精确地跟踪细菌的游泳路径。 跟踪形成为椭圆形状并在与固体表面平行的固体表面和虚拟表面之间形成的游泳空间中游动的细菌的游泳路径的方法包括以下步骤：将荧光基因转染到细菌中; 在游泳区内安排细菌游泳; 完全反射照射到固体表面的光，并在游泳空间中形成消逝场; 在细菌游泳的同时拍摄表现荧光基因的细菌，其在ev逝场中发光，并在各时刻获得表达荧光基因的细菌的图像; 并将在各时刻获得的细菌的图像拟合成椭圆形状，并将细菌的形状设定为椭圆形状和细菌相对于固体表面的位置。