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公开(公告)号:US20180363028A1
公开(公告)日:2018-12-20
申请号:US16007274
申请日:2018-06-13
申请人: Satoshi IZUMI , Manabu SEO , Takahiko MATSUMOTO , Hiroki SOMADA , Ryuya MASHIKO , Takeshi ORITO , Hiroki NAKAE
发明人: Satoshi IZUMI , Manabu SEO , Takahiko MATSUMOTO , Hiroki SOMADA , Ryuya MASHIKO , Takeshi ORITO , Hiroki NAKAE
IPC分类号: C12Q1/6806 , C12Q1/686 , G01N15/14
摘要: Provided is a method for producing a cell contained base, the method being capable of providing a cell contained base highly accurately controlled in number of nucleic acid molecules contained in a low-concentration nucleic acid standard sample, the method including a liquid droplet discharging step of discharging a cell suspension in the form of a liquid droplet with a liquid droplet discharging unit onto a base including at least one cell contained region; a cell number counting step of counting a number of cells contained in the liquid droplet with a plurality of sensors from two or more directions while the liquid droplet is flying into the cell contained region; and a liquid droplet landing step of landing the liquid droplet in the at least one cell contained region in a manner that a predetermined number of cells are located in the at least one cell contained region.