公开(公告)号：US08772484B2

公开(公告)日：2014-07-08

申请号：US13412682

申请日：2012-03-06

申请人： Satoshi Inouye ,  Yuiko Miura ,  Suguru Yoshida ,  Takamitsu Hosoya

发明人： Satoshi Inouye ,  Yuiko Miura ,  Suguru Yoshida ,  Takamitsu Hosoya

IPC分类号： C07D487/12 ,  C07D241/10

CPC分类号： C07D241/38 ,  C07D241/20 ,  C07D241/44 ,  C07D241/54 ,  C07D487/04 ,  Y02P20/55

摘要： A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

摘要翻译： 已经需要制备v-肠溶纤维素化合物的简单方法。 本发明描述了一种制备由通式（II）表示的β-环糊精化合物的方法，包括（1）在碱存在下使通式（Ⅷ）化合物与甲基三苯基鏻盐反应，得到由 通式（IX），（2）对选自由通式（IX）表示的化合物和作为化合物的通式（X）的化合物的任何一种进行闭环复分解反应的步骤 的通式（IX）的化合物，其中氨基被R 5保护，然后使R 4和（如果有的话）R 5脱保护，得到由通式（XIV）表示的v-内网络胺化合物，和（3） 通式（XIV）与通式（XV）表示的化合物反应，得到通式（II）的化合物。

公开(公告)号：US20120232272A1

公开(公告)日：2012-09-13

申请号：US13412682

申请日：2012-03-06

申请人： Satoshi INOUYE ,  Yuiko Miura ,  Suguru Yoshida ,  Takamitsu Hosoya

发明人： Satoshi INOUYE ,  Yuiko Miura ,  Suguru Yoshida ,  Takamitsu Hosoya

IPC分类号： C07D487/04 ,  C07D241/38

CPC分类号： C07D241/38 ,  C07D241/20 ,  C07D241/44 ,  C07D241/54 ,  C07D487/04 ,  Y02P20/55

摘要： A simple process for producing v-coelenterazine compounds has been desired. Described is a process for producing a v-coelenterazine compound represented by general formula (II) comprising (1) the step of reacting a compound of general formula (VIII) with a methyltriphenylphosphonium salt in the presence of a base to give a compound represented by general formula (IX), (2) the step of performing a ring-closing metathesis reaction on any one selected from the group consisting of the compound represented by general formula (IX) and a compound of general formula (X) which is the compound of general formula (IX) wherein the amino is protected with R5, and then deprotecting R4 and, if any, R5 to give a v-coelenteramine compound represented by general formula (XIV), and (3) the step of reacting the compound of general formula (XIV) with a compound represented by general formula (XV) to give the compound of general formula (II).

摘要翻译： 已经需要制备v-肠溶纤维素化合物的简单方法。 本发明描述了一种制备由通式（II）表示的β-环糊精化合物的方法，包括（1）在碱存在下使通式（Ⅷ）化合物与甲基三苯基鏻盐反应，得到由 通式（IX），（2）对选自由通式（IX）表示的化合物和作为化合物的通式（X）的化合物的任何一种进行闭环复分解反应的步骤 的通式（IX）的化合物，其中氨基被R 5保护，然后使R 4和（如果有的话）R 5脱保护，得到由通式（XIV）表示的v-内网络胺化合物，和（3） 通式（XIV）与通式（XV）表示的化合物反应，得到通式（II）的化合物。

公开(公告)号：US08816052B2

公开(公告)日：2014-08-26

申请号：US13430914

申请日：2012-03-27

申请人： Satoshi Inouye ,  Yuiko Miura

发明人： Satoshi Inouye ,  Yuiko Miura

IPC分类号： C07K1/00 ,  C07K14/435 ,  C12Q1/68 ,  C12P21/06 ,  C07H21/04

CPC分类号： C07K14/435 ,  C07K14/43595 ,  C07K2319/036 ,  C07K2319/21 ,  G01N21/6486

摘要： Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.

摘要翻译： 期望显示具有缓慢衰变的发光图案的结合钙的蛋白酶。 本发明提供了包含氨基酸序列的突变型脱辅基蛋白，其中SEQ ID NO：2的氨基酸序列中的第23至第34位氨基酸被下式I代表的氨基酸取代：Xaa23-Xaa24-Xaa25-Xaa26-Xaa27 -Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa34; 具有与肠丝氨酸的过氧化物或肠丝氨酸类似物的过氧化物结合的功能，以形成能够在钙离子的作用下发光的光蛋白; 并且具有通过光蛋白与钙离子的结合发射的发光的半衰期不少于2秒。

公开(公告)号：US20120258465A1

公开(公告)日：2012-10-11

申请号：US13430914

申请日：2012-03-27

申请人： Satoshi INOUYE ,  Yuiko Miura

发明人： Satoshi INOUYE ,  Yuiko Miura

IPC分类号： C12Q1/68 ,  C12P21/06 ,  C07H21/04 ,  C12N15/63 ,  C07K14/435 ,  C07K1/00 ,  C12Q1/02 ,  C12N1/21

CPC分类号： C07K14/435 ,  C07K14/43595 ,  C07K2319/036 ,  C07K2319/21 ,  G01N21/6486

摘要： Calcium-binding photoproteins showing the luminescence pattern with a slow decay of are desired. The invention provides a mutant apoprotein comprising an amino acid sequence wherein the 23rd to 34th amino acids in the amino acid sequence of SEQ ID NO: 2 are substituted with an amino acid represented by formula I below: Xaa23-Xaa24-Xaa25-Xaa26-Xaa27-Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa 34; having a function to bind to the peroxide of coelenterazine or the peroxide of a coelenterazine analogue to form a photoprotein capable of emitting light under the action of calcium ions; and, having a half decay time of the luminescence emitted by binding of the photoprotein to calcium ions being not less than 2 seconds.

摘要翻译： 期望显示具有缓慢衰变的发光图案的结合钙的蛋白酶。 本发明提供了包含氨基酸序列的突变型脱辅基蛋白，其中SEQ ID NO：2的氨基酸序列中的第23至第34位氨基酸被下式I代表的氨基酸取代：Xaa23-Xaa24-Xaa25-Xaa26-Xaa27 -Xaa28-Xaa29-Xaa30-Xaa31-Xaa32-Xaa33-Xaa34; 具有与肠丝氨酸的过氧化物或肠丝氨酸类似物的过氧化物结合的功能，以形成能够在钙离子的作用下发光的光蛋白; 并且具有通过光蛋白与钙离子的结合发射的发光的半衰期不少于2秒。