公开(公告)号：US20090047692A1

公开(公告)日：2009-02-19

申请号：US12099085

申请日：2008-04-07

申请人： Schuyler Boon CORRY ,  William Louis Downey ,  Brian Filanoski ,  Kyle Richard Gee ,  I. Lawrence Greenfield ,  James David Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

发明人： Schuyler Boon CORRY ,  William Louis Downey ,  Brian Filanoski ,  Kyle Richard Gee ,  I. Lawrence Greenfield ,  James David Hirsch ,  Iain Johnson ,  Aleksey Rukavishnikov

IPC分类号： G01N33/53 ,  C07D501/60 ,  C07D498/04 ,  C12Q1/34

CPC分类号： C07F5/022 ,  C07D499/00 ,  C07D501/00 ,  C07D501/58 ,  C07D501/60 ,  C07D503/00 ,  C07D519/00 ,  C12Q1/34 ,  C12Q2334/00 ,  G01N33/581 ,  G01N2333/986

摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.

摘要翻译： 本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。 特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。 除了化合物之外，公开了用于分析样品中存在β-内酰胺酶的方法，例如作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。 公开了包括公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。