公开(公告)号：US20240169179A2

公开(公告)日：2024-05-23

申请号：US17607970

申请日：2020-09-10

申请人： Science Solutions LLC ,  Consejo Nacional De investigaciones Cientificas Y Tecnicas (Conicet)

发明人： Carla Alejandra GIMENEZ ,  Guillermo Daniel REPIZO ,  Federico Alberto PEREYRA BONNET ,  Lucia Ana CURTI ,  Franco GOYTIA ,  Maria Eugenia FARIAS

IPC分类号： G06N3/00 ,  G06N10/40

CPC分类号： G06N3/002 ,  G06N10/40

摘要： Provided herein are novel Class 2 Type II and Type V CRISPR-Cas RNA-guided endonucleases and systems comprising the same. Provided also are methods of making, and methods of use thereof. Exemplary methods of use include modifying target DNAs and detecting targeting DNAs, useful for therapeutic and diagnostic applications.

公开(公告)号：US20220398426A1

公开(公告)日：2022-12-15

申请号：US17607970

申请日：2020-09-10

申请人： IONQ, INC. ,  Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet)

发明人： Carla Alejandra Gimenez ,  Guillermo Daniel REPIZO ,  Federico Alberto PEREYRA BONNET ,  Lucía Ana CURTI ,  Franco GOYTIA ,  Maria Eugenia FARÍAS

IPC分类号： G06N3/00 ,  G06N10/40

摘要： Provided herein are novel Class 2 Type II and Type V CRISPR-Cas RNA-guided endonucleases, e.g. Cas9 and Cas12 endonucleases, and systems comprising the same. Provided also are methods of making, and methods of use thereof. Exemplary methods of use include modifying target DNAs and detecting targeting DNAs, useful for therapeutic and diagnostic applications. Some of the diagnostic applications may utilise the collateral nuclease activity of an enzyme bound to a target sequence.