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1.发明申请NUCLEIC ACID SEQUENCES FROM DIABROTICA VIRGIFERA VIRGIFERA LECONTE AND USES THEREOF 审中-公开来自DIABROTICA VIRGIFERA VIRGIFERA LECONTE的核酸序列及其用途公开(公告)号：US20100192265A1
公开(公告)日：2010-07-29
申请号：US12606990
申请日：2009-10-27
CPC分类号： C07K14/43563 , C12Q1/6888
摘要： Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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2.发明授权Nucleic acid sequences from Diabrotica virgifera virgifera leconte and uses thereof 有权来自Diabrotica virgifera virgifera leconte的核酸序列及其用途公开(公告)号：US08614370B2
公开(公告)日：2013-12-24
申请号：US12606948
申请日：2009-10-27
CPC分类号： C07K14/43563 , C12Q1/6888
摘要： Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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3.发明申请NUCLEIC ACID SEQUENCES FROM DIABROTICA VIRGIFERA VIRGIFERA LECONTE AND USES THEREOF 有权来自DIABROTICA VIRGIFERA VIRGIFERA LECONTE的核酸序列及其用途公开(公告)号：US20110154545A1
公开(公告)日：2011-06-23
申请号：US12606948
申请日：2009-10-27
CPC分类号： C07K14/43563 , C12Q1/6888
摘要： Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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4.发明授权Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof 有权来自Diabrotica virgifera virgifera LeConte的核酸序列及其用途公开(公告)号：US07612194B2
公开(公告)日：2009-11-03
申请号：US10205189
申请日：2002-07-24
CPC分类号： C07K14/43563 , C12Q1/6888
摘要： Expressed Sequence Tags (ESTs) isolated from the Western Corn Rootworm, Diabrotica virgifera virgifera LeConte, are disclosed. The invention encompasses nucleic acid molecules that encode D. v. virgifera protein homologs and fragments thereof. In addition, antibodies capable of binding the proteins are encompassed by the present invention. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The invention also relates to methods of using the disclosed nucleic acid molecules, proteins, fragments of proteins, and antibodies, for example, for gene identification and analysis, and preparation of constructs.
摘要翻译： 公开了从西部玉米根虫，Diabrotica virgifera virgifera LeConte分离的表达序列标签（EST）。 本发明包括编码D.V. virgifera蛋白质同系物及其片段的核酸分子。 此外，本发明涵盖能够结合蛋白质的抗体。 所公开的EST在分离基因和启动子方面具有特别的用途，鉴定和绘制参与发育和代谢途径的基因以及确定基因功能。 EST为靶基因和分离新基因提供了独特的分子工具，用于植物保护和改良。 本发明还涉及使用所公开的核酸分子，蛋白质，蛋白质片段和抗体的方法，例如用于基因鉴定和分析以及构建体的制备。
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5.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物公开(公告)号：US07705215B1
公开(公告)日：2010-04-27
申请号：US08113561
申请日：1993-08-25
申请人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
发明人： Thomas R. Adams , Paul C. Anderson , Sheryl A. Chambers , Paul S. Chomet , Richard J. Daines , Christopher E. Flick , Kimberly Glassman , William J. Gordon-Kamm , Albert P. Kausch , Lucille B. Laccetti , Peggy G. Lemaux , Catherine J. Mackey , Mary Lou Mangano , Michael T. Mann , James V. O'Brien , Emil M. Orozco , Peter Orr , Thomas B. Rice , T. Michael Spencer , William G. Start , Michael A. Stephens , Clayton S. Vetsch , David A. Walters , Donald S. Walters , Nancy G. Willetts , Susan J. Zachwieja
CPC分类号： C12N15/8241 , A01H4/00 , A01H4/001 , C07K14/415 , C12N5/0025 , C12N5/04 , C12N9/0006 , C12N9/88 , C12N15/8207 , C12N15/8209 , C12N15/87
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
摘要翻译： 本发明涉及用于生产稳定的遗传转化的玉米细胞的可再现系统，以及选择已经转化的细胞的方法。 公开的一种选择方法使用通过微粒轰击引入的链霉菌棒基因，其在悬浮培养物中生长，然后暴露于除草剂双乙二胺磷的胚发生玉米细胞中。 实现本文公开的稳定转化的方法包括组织培养方法和培养基，用所需转化DNA轰击受体细胞的方法，以及从转化细胞生长可育植物的方法。 本发明还涉及转化的细胞和种子以及从转化细胞和其花粉生长的可育植物。
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