公开(公告)号：US06331431B1

公开(公告)日：2001-12-18

申请号：US08563654

申请日：1995-11-28

申请人： Scott M. Glaser ,  William D. Huse ,  William P. MacConnell

发明人： Scott M. Glaser ,  William D. Huse ,  William P. MacConnell

IPC分类号： B01D3500

CPC分类号： C12N1/06 ,  C12N1/005

摘要： A high throughput device is provided for simultaneously isolating periplasmic fractions from multiple samples of host cells. The device can be formatted to operate in a series. A method for simultaneously isolating multiple periplasmic fractions is also provided. The method includes (a) providing a high throughput device for isolating periplasmic fractions from multiple samples of host cells having an outer membrane via attaching a removable sample chamber having a membrane chamber wall to a vacuum chamber and applying a vacuum thereto (b) removing media with the vacuum and (c) removing the outer membrane of the host cells (d) attaching a collection chamber to the vacuum chamber and applying a vacuum thereto in order to carry out a collection of the periplasmic fractions that pass through the membrane of the chamber wall from the host cells. A workstation is also provided which includes one or more high throughput devices for simultaneously isolating periplasmic space fractions from a plurality of samples of host cells, and a device for simultaneously culturing a plurality of samples. preferably the membrane of the chamber is a neutral nylon membrane having an average pore diameter of 1 to 1.2 micron.

摘要翻译： 提供了高通量装置，用于同时从宿主细胞的多个样品中分离周质部分。 该设备可以格式化为一系列操作。 还提供了同时分离多种周质级分的方法。 该方法包括（a）提供高通量装置，用于通过将具有膜室壁的可拆卸样品室附接到真空室并向其施加真空（b）去除介质，从而从具有外膜的宿主细胞的多个样品中分离周质级分 以及（c）去除宿主细胞的外膜（d）将收集室附接到真空室并向其中施加真空，以便进行通过腔室膜的周质部分的收集 墙壁从宿主细胞。 还提供了一种工作站，其包括一个或多个用于同时从多个宿主细胞样本中隔离周质空间分数的高通量装置，以及用于同时培养多个样品的装置。 优选室的膜是平均孔径为1至1.2微米的中性尼龙膜。

公开(公告)号：US06590079B2

公开(公告)日：2003-07-08

申请号：US08791391

申请日：1997-01-30

申请人： William D. Huse ,  Scott M. Glaser

发明人： William D. Huse ,  Scott M. Glaser

IPC分类号： C07K1628

CPC分类号： C07K16/2848 ,  A61K38/00 ,  C07K2317/24 ,  C07K2319/00

摘要： The invention provides a Vitaxin antibody and a LM609 grafted antibody exhibiting selective binding affinity to &agr;v&bgr;3. The Vitaxin antibody consists of at least one Vitaxin heavy chain polypeptide and at least one Vitaxin light chain polypeptide or functional fragments thereof. Also provided are the Vitaxin heavy and light chain polypeptides and functional fragments. The LM609 grafted antibody consists of at least one CDR grafted heavy chain polypeptide and at least one CDR grafted light chain polypeptide or functional fragment thereof. Nucleic acids encoding Vitaxin and LM609 grafted heavy and light chains as well as nucleic acids encoding the parental non-human antibody LM609 are additionally provided. Functional fragments of such encoding nucleic acids are similarly provided.

摘要翻译： 本发明提供了一种Vitaxin抗体和显示对α2β3选择性结合亲和力的LM609接枝抗体。 维生素A抗体由至少一种维生素原重链多肽和至少一种维生素C轻链多肽或其功能片段组成。 还提供了维生素原重链和轻链多肽和功能片段。 LM609接枝抗体由至少一个CDR移植的重链多肽和至少一个CDR接枝的轻链多肽或其功能片段组成。 编码维生素和LM609接枝的重链和轻链的核酸以及编码亲本非人抗体LM609的核酸。 类似地提供了这种编码核酸的功能性片段。

公开(公告)号：US07422745B2

公开(公告)日：2008-09-09

申请号：US10463847

申请日：2003-06-16

申请人： William D. Huse ,  Scott M. Glaser

发明人： William D. Huse ,  Scott M. Glaser

IPC分类号： A61K39/395 ,  C07K16/28

CPC分类号： C07K16/2848 ,  A61K38/00 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2319/00

摘要： The invention provides a Vitaxin antibody and a LM609 grafted antibody exhibiting selective binding affinity αvβ3. The Vitaxin antibody consists of at least one Vitaxin heavy chain polypeptide and at least one Vitaxin light chain polypeptide or functional fragments thereof. Also provided are the Vitaxin heavy and light chain polypeptides and functional fragments. The LM609 grafted antibody consists of at least one CDR grafted heavy chain polypeptide and at least one CDR grafted light chain polypeptide or functional fragment thereof. The invention additionally provides a high affinity LM609 grafted antibody comprising one or more CDRs having at least one amino acid substitution, where the αvβ3 binding activity of the high affinity LM609 grafted antibody is enhanced. Nucleic acids encoding Vitaxin and LM609 grafted heavy and light chains as well as nucleic acids encoding the parental non-human antibody LM609 are additionally provided. Functional fragments of such encoding nucleic acids are similarly provided. The invention also provides a method of inhibiting a function of αvβ3. The method consists of contacting αvβ3 with Vitaxin or a LM609 grafted antibody or functional fragments thereof under conditions which allow binding to αvβ3. Finally, the invention provides for a method of treating an αvβ3-mediated disease. The method consists of administering an effective amount of Vitaxin or a LM609 grafted antibody or functional fragment thereof under conditions which allow binding to αvβ3.

摘要翻译： 本发明提供了一种Vitaxin抗体和显示出选择性结合亲和力的LM609接枝抗体α3β3。 维生素A抗体由至少一种维生素原重链多肽和至少一种维生素C轻链多肽或其功能片段组成。 还提供了维生素原重链和轻链多肽和功能片段。 LM609接枝抗体由至少一个CDR移植的重链多肽和至少一个CDR接枝的轻链多肽或其功能片段组成。 本发明另外提供了高亲和力LM609接枝抗体，其包含一个或多个具有至少一个氨基酸取代的CDR，其中高亲和力LM609的α3β3β3结合活性 接枝抗体增强。 编码维生素和LM609接枝的重链和轻链的核酸以及编码亲本非人抗体LM609的核酸。 类似地提供了这种编码核酸的功能性片段。 本发明还提供了抑制α3β3的功能的方法。 该方法包括在允许结合α睾丸激素的条件下，将α3β3β3和Vitaxin或LM609接枝抗体或其功能片段接触 3 。 最后，本发明提供了一种治疗α3β3介导的疾病的方法。 该方法包括在允许结合α3β3的条件下施用有效量的维生素或维生素LM609接枝抗体或其功能片段。