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    Nucleic acid purification system and method
    1.
    发明授权
    Nucleic acid purification system and method 失效
    核酸纯化系统及方法

    公开(公告)号：US5217593A

    公开(公告)日：1993-06-08

    申请号：US911515

    申请日：1992-07-10

    申请人： William P. MacConnell

    发明人： William P. MacConnell

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44756 ,  G01N27/4473

    摘要： A process for the purification of DNA and the like comprises a housing having walls forming a reservoir having a chamber for containing a buffer solution, means for circulating a buffer through the reservoir, a disposable cassette within said chamber having first means including a gel for defining a first path extending between an inlet end and an outlet end, a well for introducing a bacterial sample into the path at said inlet end thereof, and a second path intersecting the first path via an elution chamber, having a collection chamber including an elution window at said outlet end, and an electrical circuit for selectively applying an electrical potential along each of the paths for selectively moving a plasmid first along the first path from the bacterial well to the elution chamber, then along the second path to the collection window at the end thereof.

    摘要翻译： 用于纯化DNA等的方法包括具有形成具有用于容纳缓冲溶液的室的储存器的壁的壳体，用于使缓冲液循环通过储存器的装置，在所述室内的一次性盒，其具有包括用于限定的凝胶的第一装置 在入口端和出口端之间延伸的第一路径，用于将细菌样品在其入口端引入路径的井以及经由洗脱室与第一路径相交的第二路径，具有包括洗脱窗口的收集室 在所述出口端，以及电路，用于选择性地沿每个路径施加电势，用于选择性地沿着从细菌孔到洗脱室的第一路径移动质粒，然后沿着第二路径到达收集窗口 结束。

    Genomic DNA Purifier
    2.
    发明申请
    Genomic DNA Purifier 审中-公开
    基因组DNA纯化器

    公开(公告)号：US20080047835A1

    公开(公告)日：2008-02-28

    申请号：US11466398

    申请日：2006-08-22

    申请人： William P. MacConnell

    发明人： William P. MacConnell

    IPC分类号： C07K1/26 ,  G01N27/00

    CPC分类号： G01N27/447

    摘要： A system and method for purifying genomic DNA requires the use of a cassette that is formed with a plurality of wells. Each well has first and second apertures that are respectively covered by a section made of an electrophoretic filter medium (e.g. agarose). In use, a lysate is loaded into the well while the cassette is submerged in a buffer fluid. A voltage cycle is then applied to alternate between forward and reverse electrophoresis in the well, to separate impurities from the lysate for purification of genomic DNA.

    摘要翻译： 用于纯化基因组DNA的系统和方法需要使用由多个孔形成的盒。 每个孔具有分别由电泳过滤介质（例如琼脂糖）制成的部分覆盖的第一和第二孔。 在使用中，将裂解物装入孔中，同时将盒浸没在缓冲液中。 然后施加电压循环以在阱中正向和反向电泳之间交替，以从裂解物中分离杂质以纯化基因组DNA。

    Bufferless electrophoresis system and method
    5.
    发明授权
    Bufferless electrophoresis system and method 失效
    无缓冲电泳系统及方法

    公开(公告)号：US5209831A

    公开(公告)日：1993-05-11

    申请号：US715392

    申请日：1991-06-14

    申请人： William P. MacConnell

    发明人： William P. MacConnell

    IPC分类号： B01D57/02 ,  G01N27/447

    CPC分类号： G01N27/44756 ,  B01D57/02 ,  G01N27/44704

    摘要： A bufferless electrophoresis system for separation of DNA or the like includes a disposable cassette comprising a housing having open ends, a three dimensional gel body in the housing for defining a path, a sample well in a top surface of the gel for introducing a sample into the path at one end thereof, an electrically conductive film closing the ends of the housing and contacting the ends of the gel, and an electrical circuit for selectively applying an electrical potential via the electrically conductive film to the ends of the gel. A holder having spaced apart opposed terminals may be used to hold the cassette and apply the electrical circuit to the cassette.

    摘要翻译： 用于分离DNA等的无缓冲电泳系统包括一次性盒，其包括具有开口端的壳体，用于限定路径的壳体中的三维凝胶体，用于将样品引入的凝胶顶表面中的样品阱 在其一端的路径，封闭壳体的端部并接触凝胶端部的导电膜，以及用于通过导电膜选择性地将电位施加到凝胶端部的电路。 可以使用具有间隔开的相对端子的保持器来保持盒子并将电路施加到盒子。

    Plasmid purification system and method
    6.
    发明授权
    Plasmid purification system and method 失效
    质粒纯化系统及方法

    公开(公告)号：US5139637A

    公开(公告)日：1992-08-18

    申请号：US668856

    申请日：1991-03-13

    申请人： William P. MacConnell

    发明人： William P. MacConnell

    IPC分类号： G01N27/447

    CPC分类号： G01N27/4473 ,  G01N27/44756

    摘要： An apparatus for the purification of DNA and the like comprises a housing having walls forming a reservoir having a plurality of chambers for containing a buffer solution means for circulating a buffer through the reservoir, a disposable cassette within said housing having first means including a gel for defining a first path extending between a first pair of the chambers, a well for introducing a bacterial sample into the path at one end thereof, and a second path intersecting the first path via an elution window at one end, having a collection window at the other end and extending between a second pair of the chambers, and an electrical circuit for selectively applying an electrical potential along each of the paths for selectively moving a plasmid first along the first path from the bacterial well to the elution window, then along the second path to the collection window at the end thereof.

    摘要翻译： 用于纯化DNA等的装置包括：壳体，其具有形成储存器的壁，所述储存器具有多个室，用于容纳用于使缓冲液循环通过储存器的缓冲溶液装置，所述壳体内的一次性盒包括第一装置， 限定在第一对室之间延伸的第一路径，用于将细菌样品在其一端引入路径的井，以及在一端经由洗脱窗与第一路径相交的第二路径，在第一路径处具有收集窗口 另一端并在第二对腔室之间延伸，以及电路，用于选择性地沿着每个路径施加电势，以选择性地将质粒首先沿着从细菌井到洗脱窗口的第一路径移动，然后沿着第二 到达收集窗口的路径。

    Vacuum device and method for isolating periplasmic fraction from cells
    7.
    发明授权
    Vacuum device and method for isolating periplasmic fraction from cells 失效
    用于从细胞中分离周质部分的真空装置和方法

    公开(公告)号：US06331431B1

    公开(公告)日：2001-12-18

    申请号：US08563654

    申请日：1995-11-28

    申请人： Scott M. Glaser ,  William D. Huse ,  William P. MacConnell

    发明人： Scott M. Glaser ,  William D. Huse ,  William P. MacConnell

    IPC分类号： B01D3500

    CPC分类号： C12N1/06 ,  C12N1/005

    摘要： A high throughput device is provided for simultaneously isolating periplasmic fractions from multiple samples of host cells. The device can be formatted to operate in a series. A method for simultaneously isolating multiple periplasmic fractions is also provided. The method includes (a) providing a high throughput device for isolating periplasmic fractions from multiple samples of host cells having an outer membrane via attaching a removable sample chamber having a membrane chamber wall to a vacuum chamber and applying a vacuum thereto (b) removing media with the vacuum and (c) removing the outer membrane of the host cells (d) attaching a collection chamber to the vacuum chamber and applying a vacuum thereto in order to carry out a collection of the periplasmic fractions that pass through the membrane of the chamber wall from the host cells. A workstation is also provided which includes one or more high throughput devices for simultaneously isolating periplasmic space fractions from a plurality of samples of host cells, and a device for simultaneously culturing a plurality of samples. preferably the membrane of the chamber is a neutral nylon membrane having an average pore diameter of 1 to 1.2 micron.

    摘要翻译： 提供了高通量装置，用于同时从宿主细胞的多个样品中分离周质部分。 该设备可以格式化为一系列操作。 还提供了同时分离多种周质级分的方法。 该方法包括（a）提供高通量装置，用于通过将具有膜室壁的可拆卸样品室附接到真空室并向其施加真空（b）去除介质，从而从具有外膜的宿主细胞的多个样品中分离周质级分 以及（c）去除宿主细胞的外膜（d）将收集室附接到真空室并向其中施加真空，以便进行通过腔室膜的周质部分的收集 墙壁从宿主细胞。 还提供了一种工作站，其包括一个或多个用于同时从多个宿主细胞样本中隔离周质空间分数的高通量装置，以及用于同时培养多个样品的装置。 优选室的膜是平均孔径为1至1.2微米的中性尼龙膜。

    Attenuated herpesvirus, herpesvirus which include foreign DNA encoding an amino acid sequence and vaccines containing same
    8.
    发明授权
    Attenuated herpesvirus, herpesvirus which include foreign DNA encoding an amino acid sequence and vaccines containing same 失效
    减毒疱疹病毒，疱疹病毒，其包括编码氨基酸序列的外源DNA和含有其的疫苗

    公开(公告)号：US5834305A

    公开(公告)日：1998-11-10

    申请号：US334428

    申请日：1994-11-04

    申请人： Mark D. Cochran ,  William P. MacConnell ,  Richard D. MacDonald ,  David E. Junker

    发明人： Mark D. Cochran ,  William P. MacConnell ,  Richard D. MacDonald ,  David E. Junker

    IPC分类号： C12N15/86

    CPC分类号： C12N15/86

    摘要： The present invention provides a hybrid, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the hybrid, nonprimate herpesvirus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus and at least one foreign DNA sequence. Also provided is an attenuated, nonprimate herpesvirus comprising DNA which includes a sequence essential for viral replication of the attenuated, nonprimate herpes-virus, at least a portion of which is present in a sequence essential for replication of a naturally-occurring nonprimate herpesvirus, from which at least a portion of a repeat sequence has been deleted. Also provided are vaccines comprising the viruses of the invention and methods of immunizing animals against various disease.

    摘要翻译： 本发明提供了一种杂合非疱疹病毒，其包含DNA，其包含杂合体病毒复制所必需的序列，非疱疹病毒，其至少一部分以天然存在的非疱疹病毒的复制必需的序列存在，并且至少 一个外来DNA序列。 还提供了一种减毒的非疱疹病毒，其包含DNA，其包括对于减毒的非疱疹病毒的病毒复制所必需的序列，其至少一部分以天然存在的非疱疹病毒的复制必需的序列存在， 其中至少一部分重复序列已被删除。 还提供了包含本发明病毒的疫苗和针对各种疾病免疫动物的方法。