公开(公告)号：US07183056B2

公开(公告)日：2007-02-27

申请号：US10697295

申请日：2003-10-31

申请人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

发明人： Se Ho Park ,  Hae Sik Yang ,  Dae Sik Lee ,  Yong Beom Shin ,  Kyu Won Kim ,  Tae Hwan Yoon ,  Sung Jin Kim ,  Yun Tae Kim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2527/113 ,  C12Q2527/101

摘要： The present invention is directed to an improved multiplex PCR method for obtaining at least two PCR products from one PCR solution. In the multiplex PCR method for having at least two DNA amplified products from a sample positioned in a PCR equipment, the object of the present invention is to provide a novel multiplex PCR method characterized in that a primer annealing temperature and an extension time be changed per cycle with constant periods. When a multiplex PCR is performed in accordance with the present invention, limitations in determining PCR conditions due to a various sizes of PCR products or dimers caused by primers can be eliminated, and time and efforts required for determining the PCR conditions to various samples can also be reduced. Further, not only refined DNAs like cDNA, genomic DNA, vector, etc, but blood can be directly used as a multiplex PCR sample, and PCR amplification reaction can be performed even with a sample having the smallest amounts.

摘要翻译： 本发明涉及一种用于从一种PCR溶液获得至少两种PCR产物的改进的多重PCR方法。 本发明的目的是提供一种新的多重PCR方法，其特征在于，引物退火温度和延长时间根据 周期不变的周期。 当根据本发明进行多重PCR时，可以消除由于由引物引起的各种大小的PCR产物或二聚体导致的PCR条件的限制的限制，并且确定各种样品的PCR条件所需的时间和努力也可以 减少 此外，不仅可以将cDNA，基因组DNA，载体等精制DNA与血液直接用作多重PCR样品，即使使用量少的样品也能进行PCR扩增反应。

公开(公告)号：US06512196B1

公开(公告)日：2003-01-28

申请号：US09665118

申请日：2000-09-20

申请人： Tae Hwan Yoon ,  Jeong Hyun Kim ,  Youn Gyoung Chang

发明人： Tae Hwan Yoon ,  Jeong Hyun Kim ,  Youn Gyoung Chang

IPC分类号： B23K2614

CPC分类号： C03B33/076 ,  B23K26/0823 ,  B23K26/16 ,  B23K26/364 ,  B23K26/40 ,  B23K2103/50 ,  B65G2249/04 ,  C03B33/03 ,  C03B33/091 ,  G02F1/1333 ,  Y02P40/57

摘要： Laser cutting device for cutting a glass substrate of a liquid crystal display, and methods for cutting a glass substrate using that device. The device includes a vacuum chuck rotatably mounted for securing bonded first and second substrates, a rotating member for rotating the vacuum chuck, and a laser for directing a laser beam onto the first and second substrates into cut the substrates held by the vacuum chuck.

摘要翻译： 用于切割液晶显示器的玻璃基板的激光切割装置，以及使用该装置切割玻璃基板的方法。 该装置包括可旋转地安装用于固定结合的第一和第二基板的真空卡盘，用于旋转真空卡盘的旋转构件和用于将激光束引导到第一和第二基板上的激光，以切割由真空卡盘保持的基板。