公开(公告)号：US20230287322A1

公开(公告)日：2023-09-14

申请号：US18016648

申请日：2021-07-27

申请人： Seagen Inc.

发明人： Matthew John LEITH ,  Cheng-Wei Aaron CHEN ,  John Devachariam BUSHEY ,  Swapnil BHARGAVA

IPC分类号： C12M1/00 ,  C12P21/02 ,  B01D61/14 ,  B01D69/02

CPC分类号： C12M29/04 ,  C12P21/02 ,  C12M47/10 ,  C12M29/10 ,  B01D61/149 ,  B01D69/02 ,  B01D2315/10 ,  B01D2311/04 ,  B01D61/147 ,  B01D61/145 ,  B01D2325/02834 ,  B01D2325/34

摘要： Provided herein are methods and systems for production (e.g., batch production) of a polypeptide product via cell culture. In some embodiments, the methods and systems use a first bioreactor (e.g., for cell culturing), an alternating tangential flow (ATF) microfilter (e.g., for removing a polypeptide product and culture medium from the cell culture while retaining cells), a second bioreactor (e.g., for concentrating the product), and an ATF ultrafilter (e.g., for retaining product in the second bioreactor and allowing culture medium to exit).