公开(公告)号：US20140017768A1

公开(公告)日：2014-01-16

申请号：US13996305

申请日：2011-12-09

申请人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

发明人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

IPC分类号： C12N15/81

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/92 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

摘要： Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. When grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of axylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

摘要翻译： 本文公开了一种新发现的问题和解决方案，用于工程酿酒酵母发酵木糖以制备使用木糖异构酶的乙醇以将木糖转化为木酮糖，以通过木酮糖激酶进入戊糖磷酸途径。 当在含有木糖木糖醇的培养基上生长时，尽管在酿酒酵母中没有木糖还原酶活性，但是在细胞中趋于积聚。 木糖醇抑制木糖异构酶的活性。 所描述的一种溶液是同时表达外源性木糖异构酶外源木糖醇脱氢酶，而在不存在木糖还原酶表达的情况下，任选也可以过表达木酮糖激酶。 另一种溶液是来自脆弱拟杆菌的木糖异构酶，木糖异构酶比其它木糖异构酶抑制较少，木糖异构酶例如是大肠杆菌木糖异构酶。 脆弱拟杆菌木糖异构酶的表达可以单独使用，或与木糖醇脱氢酶的表达组合使用，任选地过度表达木酮糖激酶以改善从木糖产生乙醇。

公开(公告)号：US09365857B2

公开(公告)日：2016-06-14

申请号：US13996305

申请日：2011-12-09

申请人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

发明人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

IPC分类号： C12P7/06 ,  C12P7/10 ,  C12N9/90 ,  C12N9/04 ,  C12N1/19 ,  C12N15/81 ,  C12N9/92

CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/92 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

摘要： Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

摘要翻译： 本文公开了一种新发现的问题和解决方案，用于工程酿酒酵母发酵木糖以制备乙醇，利用木糖异构酶将木糖转化成木酮糖，通过木酮糖激酶进入戊糖磷酸途径。 问题是，当在含有木糖木糖醇的培养基上生长时，尽管在酿酒酵母中不存在木糖还原酶，但仍倾向于在细胞中积累。 木糖醇抑制木糖异构酶的活性。 所描述的一种溶液是同时表达外源性木糖异构酶外源木糖醇脱氢酶，而在不存在木糖还原酶表达的情况下，任选也可以过表达木酮糖激酶。 另一种溶液是来自脆弱拟杆菌的木糖异构酶，木糖异构酶比其它木糖异构酶抑制较少，木糖异构酶例如是大肠杆菌木糖异构酶。 脆弱拟杆菌木糖异构酶的表达可以单独使用，或与木糖醇脱氢酶的表达和任选地过度表达木酮糖激酶一起使用以改善从木糖产生乙醇。

公开(公告)号：US20070015261A1

公开(公告)日：2007-01-18

申请号：US11471173

申请日：2006-06-20

申请人： John D'Elia ,  Sean Jordan

发明人： John D'Elia ,  Sean Jordan

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12P13/08 ,  C12P13/06 ,  C12P13/12

摘要： The invention provides microbial strains possessing improved properties for production of aspartate-derived amino acids and chemicals. Methods of making such strains are provided. These methods include altering expression of the aceBAK operon, the glcB gene, or both. Alteration of expression may be accomplished through increased transcription, relief from native transcriptional control, and/or other means. Replacement of native promoters for these genes is also contemplated; for instance, their native promoters may be replaced by the tac promoter (Ptac).

摘要翻译： 本发明提供具有改进的产生天冬氨酸衍生的氨基酸和化学物质性质的微生物菌株。 提供制备这种菌株的方法。 这些方法包括改变aceBAK操纵子，glcB基因或两者的表达。 表达的改变可以通过增加转录，从天然转录控制和/或其他方式的缓解来实现。 也考虑替换这些基因的天然启动子; 例如，其天然启动子可以被tac启动子（Ptac）替代。

公开(公告)号：US20060242005A1

公开(公告)日：2006-10-26

申请号：US11454509

申请日：2006-06-16

申请人： Rodney Rodrigue ,  John DuBois ,  Maria Alfano ,  Dennis Grogan ,  Jennifer Comeau ,  Sean Jordan ,  David Hess ,  John Westberg ,  Lisa Westberg ,  Anthony Perna ,  Diana Morrissey ,  Jerome Nelligan

发明人： Rodney Rodrigue ,  John DuBois ,  Maria Alfano ,  Dennis Grogan ,  Jennifer Comeau ,  Sean Jordan ,  David Hess ,  John Westberg ,  Lisa Westberg ,  Anthony Perna ,  Diana Morrissey ,  Jerome Nelligan

IPC分类号： G06F11/34

CPC分类号： G09B19/0053 ,  G06Q10/06316 ,  G06Q10/0633 ,  G06Q10/06398

摘要： A comprehensive method to train and aid manufacturers in identifying inefficiencies in product manufacturing processes and in improving such processes. The method includes placing individuals in roles associated with a complete manufacturing operation from supply acquisition through to product delivery in the simulation of a product to be made. The method includes a set of tools describing individual stages of the complete manufacturing process but with all tools related to the common simulation. The individuals carry out their roles in the steps of the production simulation and their roles may be varied. This comprehensive method aids individuals in the observation and correction of wasteful production activities from the supply chain through manufacturing and back-end functions.

摘要翻译： 一种综合的方法来训练和帮助制造商识别产品制造过程中的低效率和改进这些过程。 该方法包括将个人与完整的制造操作相关联的角色，从供应获取到产品交付在要制作的产品的模拟中。 该方法包括一组描述完整制造过程的各个阶段的工具，但是具有与常见模拟相关的所有工具。 个人在生产模拟步骤中发挥作用，其作用可能有所不同。 这种综合方法有助于个人通过制造和后端功能观察和纠正供应链中的浪费生产活动。

公开(公告)号：US20060205044A1

公开(公告)日：2006-09-14

申请号：US11371732

申请日：2006-03-09

申请人： John D'Elia ,  Sean Jordan

发明人： John D'Elia ,  Sean Jordan

IPC分类号： C12P13/04 ,  C12P13/08 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12P13/06 ,  C12P13/12

摘要： The invention relates to novel bacterial strains and constructs as well as methods for production of L-amino acids, including but not limited to L-threonine. Such novel bacterial strains may be characterized by, for instance, Escherichia coli strains in which an aspartate semialdehyde dehydrogenase (asd) gene is operably associated with at least one non-native promoter, non-native ribosome binding site, or both.

摘要翻译： 本发明涉及新的细菌菌株和构建体以及生产L-氨基酸的方法，包括但不限于L-苏氨酸。 这样的新型细菌菌株的特征可以是例如其中天冬氨酸半醛脱氢酶（asd）基因与至少一种非天然启动子，非天然核糖体结合位点或两者可操作地相关的大肠杆菌菌株。