公开(公告)号：US20060115885A1

公开(公告)日：2006-06-01

申请号：US10549260

申请日：2004-03-10

申请人： Seiichi Toki ,  Hiroaki Ichikawa ,  Keishi Osakabe

发明人： Seiichi Toki ,  Hiroaki Ichikawa ,  Keishi Osakabe

IPC分类号： A01H1/00

CPC分类号： C12N15/8213

摘要： The present inventors conceived that a marker gene may be efficiently and easily removed by using the floral dip method to transiently express high levels of a site-specific recombinase gene. it is considered that the present methods can also be applied to methods for transposing a transposon that does not have a transposase from a transformed plant that comprises the transposon having no transposase.

摘要翻译： 本发明人构想出通过使用花浸法瞬时表达高水平的位点特异性重组酶基因可以有效且容易地除去标记基因。 认为本发明的方法也可以应用于从不含转座子的转化植物转座不具有转座酶的转座子的方法。

公开(公告)号：US20130074220A1

公开(公告)日：2013-03-21

申请号：US13504034

申请日：2010-10-25

申请人： Seiichi Toki ,  Keishi Osakabe

发明人： Seiichi Toki ,  Keishi Osakabe

IPC分类号： C12N15/01

CPC分类号： C12N15/01 ,  A01H1/06 ,  C12N15/8213

摘要： It has been found that the use of a plant cell in which a function of a protein involved in repair by nonhomologous end joining is artificially suppressed dramatically increases the efficiency of introductions of non-silent mutations in a repairing process by nonhomologous end joining which occurs after induction of a DNA double-strand break with a zinc finger nuclease.

摘要翻译： 已经发现，使用通过非同源末端连接进行修复的蛋白质的功能被人为抑制的植物细胞显着地增加了在修复过程中通过非同源末端连接引入非沉默突变的效率，其发生在 用锌指核酸酶诱导DNA双链断裂。

公开(公告)号：US07238864B2

公开(公告)日：2007-07-03

申请号：US11075808

申请日：2005-03-10

申请人： Seiichi Toki ,  Hiroaki Ichikawa ,  Hidemitsu Nakamura ,  Kiyoshi Kawai ,  Koichiro Kaku ,  Tsutomu Shimizu

发明人： Seiichi Toki ,  Hiroaki Ichikawa ,  Hidemitsu Nakamura ,  Kiyoshi Kawai ,  Koichiro Kaku ,  Tsutomu Shimizu

IPC分类号： C07H21/04 ,  C12N5/05 ,  C12N15/09 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N15/8274 ,  C12N15/8222

摘要： The present invention provides methods for inducing tissue-specific expression, which includes preparing an expression vector having a promoter. The promoter includes any one of the following DNAs (a) to (c): (a) a DNA, which includes the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which includes the nucleotide sequence ranging from nucleotides 1344 to 2843 of SEQ ID NO: 1; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment including a nucleotide sequence fully complementary to the nucleotide sequence shown in SEQ ID NO: 1, wherein (a), (b) or (c) can control the transcription of a coding sequence located downstream thereof; and wherein the stringent conditions are 5×SSC at 42° C.; The expression vector, also, includes a coding sequence located downstream of the promoter. Additionally, the method includes introducing the expression vector into a plant cell to obtain a transgenic plant; wherein the promoter activity is lower in mature seeds or root bases than in plant tissues other than mature seeds or root bases. The present invention also provides transformants containing a promoter; the promoter includes any of the DNAs (a) to (c) described above.

摘要翻译： 本发明提供诱导组织特异性表达的方法，其包括制备具有启动子的表达载体。 启动子包括以下DNA（a）至（c）中的任一种：（a）包含SEQ ID NO：1所示核苷酸序列的DNA; （b）DNA，其包含从SEQ ID NO：1的核苷酸1344至2843的核苷酸序列; 和（c）DNA，其是在严格条件下与包含与SEQ ID NO：1所示的核苷酸序列完全互补的核苷酸序列的DNA片段杂交的DNA片段，其中，（a），（b）或（ c）可以控制位于其下游的编码序列的转录; 并且其中严格条件在42℃为5×SSC; 表达载体也包括位于启动子下游的编码序列。 此外，该方法包括将表达载体导入植物细胞以获得转基因植物; 其中成熟种子或根系中的启动子活性低于在成熟种子或根基以外的植物组织中的活性。 本发明还提供含有启动子的转化体; 启动子包括上述DNA（a）至（c）中的任一种。

公开(公告)号：US20050241021A1

公开(公告)日：2005-10-27

申请号：US11075808

申请日：2005-03-10

申请人： Seiichi Toki ,  Hiroaki Ichikawa ,  Hidemitsu Nakamura ,  Kiyoshi Kawai ,  Koichiro Kaku ,  Tsutomu Shimizu

发明人： Seiichi Toki ,  Hiroaki Ichikawa ,  Hidemitsu Nakamura ,  Kiyoshi Kawai ,  Koichiro Kaku ,  Tsutomu Shimizu

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82

CPC分类号： C12N15/8274 ,  C12N15/8222

摘要： The present invention enables characteristic gene expression control and relates to a promoter, which comprises any one of the following DNAs (a) to (c): (a) a DNA, which comprises the nucleotide sequence shown in SEQ ID NO: 1; (b) a DNA, which comprises a nucleotide sequence derived from the nucleotide sequence shown in SEQ ID NO: 1 by deletion, substitution, insertion, or addition of 1 or a plurality of nucleotides, and which can control transcription of a gene located downstream thereof; and (c) a DNA, which is a DNA fragment that can hybridize under stringent conditions to a DNA fragment comprising a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO: 1, and which can control the transcription of a gene located downstream thereof.

摘要翻译： 本发明能够进行特征性基因表达控制，涉及包含以下DNA（a）〜（c）中的任一种的启动子：（a）包含SEQ ID NO：1所示的核苷酸序列的DNA。 （b）DNA，其包含通过缺失，取代，插入或添加1个或多个核苷酸而衍生自SEQ ID NO：1所示核苷酸序列的核苷酸序列，并且其可以控制位于下游的基因的转录 的; 和（c）DNA，其是可以在严格条件下与包含与SEQ ID NO：1所示的核苷酸序列互补的核苷酸序列的DNA片段杂交的DNA片段，其可以控制位于下游的基因的转录 其中。

公开(公告)号：US07544858B2

公开(公告)日：2009-06-09

申请号：US10594130

申请日：2005-03-25

申请人： Seiichi Toki

发明人： Seiichi Toki

IPC分类号： C12N15/82

CPC分类号： C12N15/8205

摘要： Provided is a method of transforming a monocotyledon by using agrobacterium. There is provided a method of transforming a monocotyledon by using agrobacterium containing a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed with agrobacterium. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

摘要翻译： 提供了使用农杆菌转化单子叶植物的方法。 提供了通过使用含有所需重组基因的农杆菌来转化单子叶植物的方法。 该转化方法包括以下步骤：将含有植物生长因子的培养基与单子叶种子播种，进行1〜3天的培养，以便用农杆菌发芽和感染种子。 这种方法使得可以快速转化单子叶植物，包括水稻植物。

公开(公告)号：US20070256188A1

公开(公告)日：2007-11-01

申请号：US10594130

申请日：2005-03-25

申请人： Seiichi Toki

发明人： Seiichi Toki

IPC分类号： C12N15/82

CPC分类号： C12N15/8205

摘要： A method of transforming a monocotyledon by means of agrobacterium. There is provided a method of transforming a monocotyledon by means of agrobacterium containing a desired recombinant gene. This transforming method comprises the steps of sowing a culture medium containing a plant growth factor with a monocotyledonous seed, conducting culturing for 1 to 3 days so as to effect germination and infecting the seed with agrobacterium. This method makes it feasible to rapidly transform monocotyledons, including a rice plant.

摘要翻译： 通过农杆菌转化单子叶植物的方法。 提供了通过含有所需重组基因的农杆菌转化单子叶植物的方法。 该转化方法包括以下步骤：将含有植物生长因子的培养基与单子叶种子播种，进行1〜3天的培养，以便用农杆菌发芽和感染种子。 这种方法使得可以快速转化单子叶植物，包括水稻植物。

公开(公告)号：US06831217B1

公开(公告)日：2004-12-14

申请号：US09003072

申请日：1998-01-05

申请人： Makoto Matsuoka ,  Mitsue Tokutomi ,  Seiichi Toki ,  Maurice Sun-Ben Ku

发明人： Makoto Matsuoka ,  Mitsue Tokutomi ,  Seiichi Toki ,  Maurice Sun-Ben Ku

IPC分类号： A01H100

CPC分类号： C12N15/8243 ,  C12N9/88 ,  C12N15/8269

摘要： A C3 plant has a gene for an enzyme involved in a C4 pathway of photosynthesis and expresses the C4 photosynthesis gene at a high level. More specifically, the C3 plant includes DNA which contains (a) an expression control region of a gene for an enzyme involved in a photosynthetic pathway of a phylogenetically related C4 plant and (b) a structural gene for an enzyme involved in a photosynthetic pathway of the C4 plant. The C3 plant expresses the enzyme encoded by the structural gene at a high level.