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公开(公告)号:US20050223432A1
公开(公告)日:2005-10-06
申请号:US11000752
申请日:2004-12-01
申请人: Shane Abbitt , William Gordon-Kamm , Keith Lowe , Peizhong Zheng
发明人: Shane Abbitt , William Gordon-Kamm , Keith Lowe , Peizhong Zheng
CPC分类号: C12N15/8234 , C07K14/415
摘要: Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for promoter sequences isolated from the maize Ovule Development Protein 2 (ODP2) gene. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is further provided. The method comprises introducing into a plant or plant cell an expression cassette comprising an ODP2 promoter of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in regulating expression of nucleotide sequences of interest in a seed-preferred manner. Transformed plants, plant cells, and seeds comprising the ODP2 promoter sequence or variants and fragments thereof are also provided.
摘要翻译: 提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物包括用于从玉米Ovule发育蛋白2(ODP2)基因分离的启动子序列的新型核酸分子及其变体和片段。 还提供使用本文公开的启动子序列在植物中表达目的核苷酸序列的方法。 该方法包括向植物或植物细胞中引入包含可操作地连接到感兴趣的核苷酸序列的本发明的ODP2启动子的表达盒。 特别地,组合物和方法可用于以种子优选的方式调节感兴趣的核苷酸序列的表达。 还提供了转化的植物,植物细胞和包含ODP2启动子序列或其变体和片段的种子。
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公开(公告)号:US20070220626A1
公开(公告)日:2007-09-20
申请号:US11519184
申请日:2006-09-11
申请人: Shane Abbitt , William Gordon-Kamm , Keith Lowe
发明人: Shane Abbitt , William Gordon-Kamm , Keith Lowe
CPC分类号: C12Y505/01004 , C07K14/415 , C12N9/90 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for callus-tissue and seed embryo-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant or callus tissue using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the regulatory sequences of the present invention and regenerating a stably transformed plant or callus tissue from the transformed plant cell.
摘要翻译: 本发明提供用于调节植物中分离的核苷酸序列表达的组合物和方法。 组合物是用于愈伤组织和种子胚优选调节序列的新型核酸序列。 还提供了使用调节序列在植物或愈伤组织中表达分离的核苷酸序列的方法。 所述方法包括将植物细胞转化成含有与本发明的调控序列可操作地连接的分离的核苷酸序列,并从转化的植物细胞再生稳定转化的植物或愈伤组织。
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3.
公开(公告)号:US07897746B2
公开(公告)日:2011-03-01
申请号:US12437968
申请日:2009-05-08
申请人: Shane Abbitt , Ramesh Nair
发明人: Shane Abbitt , Ramesh Nair
CPC分类号: C12N15/8235 , C07K14/415 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter and terminator isolated from the maize lipid transfer protein 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是分离自玉米脂质转移蛋白1编码区的果皮优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号:US20100281570A1
公开(公告)日:2010-11-04
申请号:US12773450
申请日:2010-05-04
申请人: Shane Abbitt , Jinrui Shi
发明人: Shane Abbitt , Jinrui Shi
CPC分类号: C07K14/415 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter associated with the maize 18 KD OLE (18 kilodalton oleosin) coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.
摘要翻译: 本发明提供用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物是与玉米18KD OLE(18千道尔油质蛋白)编码区相关的种子优选启动子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达目标核苷酸序列的方法。
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公开(公告)号:US07550579B2
公开(公告)日:2009-06-23
申请号:US11408223
申请日:2006-04-20
申请人: Shane Abbitt , Ramesh Nair
发明人: Shane Abbitt , Ramesh Nair
CPC分类号: C12N15/8235 , C07K14/415 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter and terminator isolated from the maize lipid transfer protein 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是分离自玉米脂质转移蛋白1编码区的果皮优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号:US20070174935A1
公开(公告)日:2007-07-26
申请号:US11408223
申请日:2006-04-20
申请人: Shane Abbitt , Ramesh Nair
发明人: Shane Abbitt , Ramesh Nair
CPC分类号: C12N15/8235 , C07K14/415 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter and terminator isolated from the maize lipid transfer protein 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是分离自玉米脂质转移蛋白1编码区的果皮优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号:US08481811B2
公开(公告)日:2013-07-09
申请号:US12771177
申请日:2010-04-30
申请人: Shane Abbitt
发明人: Shane Abbitt
CPC分类号: C07K14/8139 , C12N15/8233 , C12N15/8234 , C12N15/8235
摘要: The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter associated with the maize cystatin coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.
摘要翻译: 本发明提供用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物是与玉米半胱氨酸蛋白酶抑制剂编码区相关的果皮优选启动子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达目标核苷酸序列的方法。
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公开(公告)号:US20100281569A1
公开(公告)日:2010-11-04
申请号:US12773411
申请日:2010-05-04
申请人: Shane Abbitt , Jinrui Shi
发明人: Shane Abbitt , Jinrui Shi
CPC分类号: C12N15/8234 , C07K14/415
摘要: The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter associated with the maize 17 KD OLE (17 kilodalton oleosin) coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.
摘要翻译: 本发明提供用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。 组合物是与玉米17 KD OLE(17千道尔油质蛋白)编码区相关的种子优选启动子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达目标核苷酸序列的方法。
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公开(公告)号:US20090265806A1
公开(公告)日:2009-10-22
申请号:US12463022
申请日:2009-05-08
申请人: Shane Abbitt , Ramesh Nair
发明人: Shane Abbitt , Ramesh Nair
CPC分类号: C12N15/8235 , C07K14/415 , C12N15/8234
摘要: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a pericarp-preferred promoter and terminator isolated from the maize lipid transfer protein 1 coding region. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译: 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是分离自玉米脂质转移蛋白1编码区的果皮优选启动子和终止子的新核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号:US20060272058A1
公开(公告)日:2006-11-30
申请号:US11441863
申请日:2006-05-26
申请人: Shane Abbitt , Rudolf Jung
发明人: Shane Abbitt , Rudolf Jung
CPC分类号: C12N15/8234
摘要: The present invention provides compositions for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for seed-preferred regulatory sequences.
摘要翻译: 本发明提供用于调节植物中分离的核苷酸序列表达的组合物。 所述组合物是用于种子优选调节序列的新型核酸序列。
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