公开(公告)号：US06255048B1

公开(公告)日：2001-07-03

申请号：US09011223

申请日：1998-02-09

申请人： Shigeru Hosoi ,  Makiko Kojima ,  Sachiko Kadouchi

发明人： Shigeru Hosoi ,  Makiko Kojima ,  Sachiko Kadouchi

IPC分类号： C12Q168

CPC分类号： G01N33/582 ,  C12Q1/6834 ,  G01N33/533 ,  Y10S435/968 ,  Y10S436/828 ,  C12Q2563/107 ,  C12Q2545/114 ,  C12Q2565/601

摘要： This invention provides fluoroassay which comprises labeling analyte molecules with a fluorescent material having a nucleic acid portion stained with a sufficient number of fluorochrome molecules so as to be measurable as fluorescent spots, and a reactive group binding to the analyte molecule specifically, immobilizing the labeled analyte on a solid phase, and counting the number of fluorescent spots. The nucleic acid portion of the fluorescent labeling material is a double-stranded or single-stranded nucleic acid, and the staining with the fluorochrome molecules is performed with intercalating type, minor groove binding type, or covalently binding to the nucleic acid type.

摘要翻译： 本发明提供了荧光测定法，其包括用荧光材料标记分析物分子，该荧光材料具有用足够数量的荧光染料分子染色的核酸部分，以便可测量为荧光斑点，以及特异性结合分析物分子的反应性基团，将标记的分析物 在固相上，并计数荧光斑数。 荧光标记材料的核酸部分是双链或单链核酸，并且用插入型，小沟结合型或共价结合核酸型进行用荧光染料分子的染色。

公开(公告)号：US5919917A

公开(公告)日：1999-07-06

申请号：US11551

申请日：1998-03-02

申请人： Hirofumi Shiono ,  Hirofumi Kodama ,  Makiko Kojima

发明人： Hirofumi Shiono ,  Hirofumi Kodama ,  Makiko Kojima

IPC分类号： C07H21/00 ,  C07H21/04 ,  C12Q1/68

CPC分类号： C07H21/00

摘要： The photocleavable cyclic oligonucleotide according to the invention is the one that possesses a base sequence having the hybridization ability toward DNA or RNA to targeted, and is further provided with the structure cyclized by a photocleavable group. Accordingly, the photocleavable cyclic oligonucleotide according to the invention, after having been introduced in vivo, is hardly susceptible to the nuclease decomposition reaction owing to its cyclic structure and thus it is capable of diffusing toward the predetermined sites in vivo with sufficient time. Moreover, by being irradiated with the light at an appropriate wavelength after a predetermined period of time, the photocleavable group as described above is cleaved photochemically, thus cutting the predetermined bond. This permits the oligonucleotide that was cyclic to be a linear oligonucleotide which expresses the function of an antisense oligonucleotide.

摘要翻译： PCT No.PCT / JP97 / 01959 Sec。 371日期1998年3月2日 102（e）1998年3月2日PCT 1997年6月9日PCT公布。 公开号WO97 / 47639 1997年12月18日根据本发明的可光修复的环状寡核苷酸是具有对靶向的DNA或RNA具有杂交能力的碱基序列的碱基序列，并且还具有由可光洁化基团环化的结构。 因此，根据本发明的光可透洗性环状寡核苷酸在体内导入后，由于其环状结构，几乎不受核酸酶分解反应的影响，因此能够在足够的时间内向体内预定位置扩散。 此外，通过在预定时间后用适当波长的光照射，如上所述的光可透洗组被光电化学地切割，从而切割预定的键。 这允许循环的寡核苷酸是表达反义寡核苷酸功能的线性寡核苷酸。