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公开(公告)号：US20220089693A1
公开(公告)日：2022-03-24
申请号：US17371241
申请日：2021-07-09
申请人： Shihezi University
发明人： Chuangfu CHEN , Peng WU , Chencheng XIAO , Chengyao LI , Yong WANG , Zhen WANG
IPC分类号： C07K16/10 , G01N33/569
摘要： A nanobody and its application based on SARS-CoV-2 S protein are provided, and the present disclosure relates to biomedical technology. The present disclosure chooses the Spike S1+S2 ECD of SARS-CoV-2 as a target, and screens the nanobody against of SARS-CoV-2 by using a nanobody library. After an ELISA test, the Spike S1+S2 ECD target of SARS-CoV-2 can be specifically identified while a SPIKE RBD target is identified, and a binding signal is relatively strong. The corresponding nanobody sequence is constructed into a prokaryotic expression vector for expression and purification to express the target nanobody successfully. After the purification, the purity is greater than 90%. The ELISA test of VHH nanobody showed that the purified nanobody has higher affinity to the two targets.
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公开(公告)号：US20220089692A1
公开(公告)日：2022-03-24
申请号：US17371155
申请日：2021-07-09
申请人： Shihezi University
发明人： Chuangfu CHEN , Peng WU , Chencheng XIAO , Chengyao LI , Yong WANG , Zhen WANG
摘要： A nanobody and its application based on SARS-CoV-2 S protein S1 subunit are provided, and the present disclosure relates to biomedical technology. The present disclosure chooses the Spike RBD of SARS-CoV-2 as a target, and screens the nanobody targeting SARS-CoV-2 by using a nanobody library. After an ELISA test, the Spike RBD target of SARS-CoV-2 can be specifically identification while a SPIKE S1+S2 ECD target is identification, and a binding signal is relatively strong. The corresponding nanobody sequence is constructed into a prokaryotic expression vector for expression and purification to express the target nanobody successfully. After purification, the purity is greater than 90%. The ELISA test of VHH nanobody showed that the purified nanobody has higher affinity to the two targets.
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