公开(公告)号：US20240239717A1

公开(公告)日：2024-07-18

申请号：US17782669

申请日：2021-09-29

申请人： SHIHEZI UNIVERSITY ,  JIANGSU GUOXIN UNION ENERGY CO., LTD

发明人： FENGHUA ZHANG ,  YAGUANG ZHAO ,  FUXIN SUN ,  ZHIJIE HU ,  DONGJIAO ZHOU

IPC分类号： C05B7/00

CPC分类号： C05B7/00

摘要： The present invention discloses a compound microbial fertilizer, comprising citric acid fermentation raffinate, Arthrobacter fermentation liquid, urea, monoammonium phosphate and potassium sulphate; the proportion of each component is: (400-600) mL:(400-600) mL:(50-80) g:(50-80) g:(40-60) g; and the pH value of the compound microbial fertilizer is adjusted to 5.5-7.0 with ammonia water. The fertilizer can increase crop yield, promote crop growth, and improve the soil.

公开(公告)号：US11978190B2

公开(公告)日：2024-05-07

申请号：US17409967

申请日：2021-08-24

申请人： Shihezi University

发明人： Ruoyu Zhang ,  Long Wan ,  Yinglan Jiang ,  Mengyun Zhang ,  Jinqiang Chang ,  Fangdan Song

IPC分类号： G06T7/00 ,  B65G17/12 ,  G01N5/00 ,  G01N21/95 ,  G01N33/00 ,  H04N23/90

CPC分类号： G06T7/0004 ,  B65G17/12 ,  G01N5/00 ,  G01N21/95 ,  G01N33/0098 ,  H04N23/90 ,  G06T2207/20081

摘要： A rapid detection system for water and impurity of machine-harvested seed cotton in purchase link, including: a seed cotton large-impurity-cleaning device, a seed cotton sample case, a driving and transmission device, a cotton pressing device, an image collection and processing device, and a moisture regain rate detection device and a control cabinet. The seed cotton sample case consists of a positioning plate and a high light transmittance glass case, the weighing device consists of a weighing sensor, a pallet, and an electric push rod. The driving and transmission device consists of the rollers, the motor of conveyor belts and conveyor belts, the cotton pressing device consists of an electric push rod, a connecting rod, a connecting plate, and a high light transmittance glass plate. Said rapid detection system could realize the rapid detection to the impurity rate and moisture regain rate of the seed cotton.

公开(公告)号：US20230110670A1

公开(公告)日：2023-04-13

申请号：US17955780

申请日：2022-09-29

申请人： SHIHEZI UNIVERSITY

发明人： Jie SU ,  Jianping ZHANG ,  Zhiping CAI

IPC分类号： A01K67/033

摘要： Disclosed is a continuous feeding device for predatory mites, including a feeding part and an isolation part; the feeding part includes a plurality of feeding assemblies arranged in sequence from top to bottom, and the top and bottom ends of two adjacent feeding assemblies are detachably connected by a connector; the feeding assemblies include feeding boxes, bottom screens and supporting bases arranged in sequence from top to bottom; the bottom ends of the feeding boxes are fixedly connected with the top ends of the bottom screens, the bottom ends of the bottom screens are fixedly connected with the top ends of the supporting bases, and the bottom end of the supporting base of any feeding box is detachably connected with the top end of an adjacent feeding box through the connector; climbing pieces are arranged inside the feeding boxes; and an inducer is arranged outside the feeding boxes.

公开(公告)号：US20210002370A1

公开(公告)日：2021-01-07

申请号：US16920551

申请日：2020-07-03

申请人： Shihezi University

发明人： Chuangfu CHEN ,  Peng WU

IPC分类号： C07K16/28 ,  C12N15/10 ,  C12Q1/686

摘要： This disclosure pertains to the technical field of antibodies, and discloses PD-L1 nanobodies, preparation methods and uses thereof; two primers are designed and synthesized; a sufficient amount of target products is amplified by PCR to carry out replacement enzyme digestion; target fragments are ligated to vectors, transformed, and screened to obtain clones; proteins are identified and expressed; antibody library is constructed; M13 phage display system is selected to display VHH antibody library, which consists of pMECS phagemid vector, E. coli TG1 and M13K07 helper phage. In the phagemid vector pMECS of this disclosure, the sequence before the Pst I restriction site is the coding sequence of the pelB secretion signal peptide and part of the amino acids in the first framework region of the antibody; the pelB signal peptide can guide the secretion of the subsequent polypeptide to the periplasmic cavity; Not I restriction site is followed by the coding sequence of HA and 6×His tag, which can be used for purification or detection of fusion protein.

公开(公告)号：US20240294929A1

公开(公告)日：2024-09-05

申请号：US18522708

申请日：2023-11-29

申请人： SHIHEZI UNIVERSITY

发明人： Genlin ZHANG ,  Yuanyuan LI ,  Jinyu ZHAO ,  Di QIU ,  Yan ZHANG

IPC分类号： C12N15/81 ,  C12P13/00 ,  C12P13/02

CPC分类号： C12N15/81 ,  C12P13/001 ,  C12P13/02

摘要： The present disclosure provides engineered Saccharomyces cerevisiae strains for production of vanillylamine and capsaicin, and a construction method and use thereof, and belongs to the technical field of bioengineering. In the present disclosure, a synthesis pathway of vanillylamine is expressed heterologously in Saccharomyces cerevisiae for the first time, and biosynthesis of the vanillylamine is realized for the first time. After the optimization of precursors supply and the optimization of S-adenosyl-L-methionine (SAM) cycle, a yield of the vanillylamine reaches 16.48 g/L, and a synthesis efficiency of the vanillylamine is increased by nearly 155%. This is the highest optimization efficiency obtained by optimizing the SAM cofactor strategy so far.

公开(公告)号：US12016261B1

公开(公告)日：2024-06-25

申请号：US18594386

申请日：2024-03-04

申请人： Shihezi University

发明人： Ping Jiang ,  Laiman Su ,  JianBing Ge ,  Saiyaremu Halifu ,  Xiang Huang ,  Mengyao He

IPC分类号： A01C5/02 ,  A01C5/04 ,  A01G9/24 ,  A01G22/05

CPC分类号： A01C5/04 ,  A01C5/02 ,  A01G9/243 ,  A01G22/05

摘要： An equidistant hole marking device for blueberry cultivation in a solar greenhouse is provided, which relates to the field of hole spacing marking technologies. The device includes a trigger rod, and the trigger rod is L-shaped. Four groups of moving rods are disposed outside an inner rod, the moving rods are diamond, and the moving rods are made of a plastic material. A circular pressing member is fixed outside each group of the moving rods, an upper side and a lower side of each circular pressing member are inclined, and the circular pressing members are made of a plastic material. When discharging powder, the moving rods and the circular pressing members are controlled to move inside a storage member, so that caked powder is crushed and discharged smoothly, thereby to improve marking efficiency.

公开(公告)号：US11967182B2

公开(公告)日：2024-04-23

申请号：US18449412

申请日：2023-08-14

申请人： SHIHEZI UNIVERSITY

发明人： Jie Su ,  Chen Fang ,  Jianping Zhang ,  Jiamin Gu

IPC分类号： G06V40/20 ,  G06T7/80 ,  G06V20/40 ,  G06V20/69 ,  A01K29/00

CPC分类号： G06V40/20 ,  G06T7/80 ,  G06V20/41 ,  G06V20/693 ,  G06V20/698 ,  A01K29/005 ,  G06T2207/10016 ,  G06T2207/30188

摘要： An intelligent analysis system applied to ethology of various kinds of high-density minimal polypides is provided, including an operating platform subsystem and an analysis subsystem; the operating platform subsystem is used for providing an activity medium capable of obtaining behaviors of the minimal polypides and shooting the behaviors of the minimal polypides; the behavior collecting module in the analysis subsystem is used for obtaining an ethology video of the high-density minimal polypides through the operating platform subsystem; the analysis modeling module is used for obtaining ethology big data of various kinds of high-density minimal insects according to the ethology video, and establishing an ethology model; the simulation predicting module is used for dynamically predicting comprehensive control strategies and control effects of minimal pests in a field ecosystem according to the ethology model; the visual demonstrating module is used for displaying the above behavior data.

公开(公告)号：US20220089693A1

公开(公告)日：2022-03-24

申请号：US17371241

申请日：2021-07-09

申请人： Shihezi University

发明人： Chuangfu CHEN ,  Peng WU ,  Chencheng XIAO ,  Chengyao LI ,  Yong WANG ,  Zhen WANG

IPC分类号： C07K16/10 ,  G01N33/569

摘要： A nanobody and its application based on SARS-CoV-2 S protein are provided, and the present disclosure relates to biomedical technology. The present disclosure chooses the Spike S1+S2 ECD of SARS-CoV-2 as a target, and screens the nanobody against of SARS-CoV-2 by using a nanobody library. After an ELISA test, the Spike S1+S2 ECD target of SARS-CoV-2 can be specifically identified while a SPIKE RBD target is identified, and a binding signal is relatively strong. The corresponding nanobody sequence is constructed into a prokaryotic expression vector for expression and purification to express the target nanobody successfully. After the purification, the purity is greater than 90%. The ELISA test of VHH nanobody showed that the purified nanobody has higher affinity to the two targets.

公开(公告)号：US12075725B2

公开(公告)日：2024-09-03

申请号：US18592360

申请日：2024-02-29

申请人： SHIHEZI UNIVERSITY

发明人： Ping Jiang ,  Jianbing Ge ,  Jing Ye

IPC分类号： A01D45/00 ,  A01D43/063

CPC分类号： A01D43/0638 ,  A01D45/00

摘要： Disclosed is a mowing method for compensatory growth of a desert plant Anabasis aphylla, falling into the field of plant mowing methods. The mowing method includes the following steps: step 1: mounting a frame body on movable equipment; step 2: driving the movable equipment to move a device to a working area; and step 3: intermittently operating the movable equipment to allow an area formed by a push plate I, baffle plates II and a push plate II to be exactly opposite to a suitable number of Anabasis aphylla. The present disclosure is to provide a mowing method for compensatory growth of a desert plant Anabasis aphylla to solve the technical problem, facilitating the mowing of Anabasis aphylla.

公开(公告)号：US11884974B2

公开(公告)日：2024-01-30

申请号：US16919500

申请日：2020-07-02

申请人： Shihezi University

发明人： Chuangfu Chen ,  Peng Wu

IPC分类号： C07K16/28 ,  C12Q1/686 ,  C12Q1/6886

CPC分类号： C12Q1/686 ,  C07K16/2803 ,  C12Q1/6886 ,  C07K2317/569 ,  C07K2317/76

摘要： The application belongs to the technical field of animal or human antibodies, and discloses a TIM-3 nanobody, a preparation method thereof and use thereof. The nanobody is a TIM-3 nanobody with sequence of SEQ ID NO:1. TIM-3 antigen is a transient transfection expression by mammalian cells. TIM-3 antigen is used to screen the nanobody library repeatedly, specific phage of nanobody is obtained, and the target fragment is conducted sequencing. The application uses the HEK293 cell line to express antigen. Using the mammalian expression system to express human protein may maximumly guarantee the original structure of the protein, guarantee the protein to have a post-translational modification and specific modifications of eukaryotic proteins such as glycosylation, which makes the obtained protein have high activity. This method maximizes the original structure and activity of the protein; the nanobodies screened by the application can efficiently and specifically bind to the target.

Shihezi University
TIM-3 nanobody, a preparation method thereof, and use thereof
3
1
1049
DNA
Artificial Sequence
1
GGTGGGCGGA CATTTCACAA GCTTAAGGAG ACAGTACATA TGAAATACCT ATTGCCTACG GCAGCCGCTG GATTGTTATT ACTCGCGGCC CAGCCGGCCA TGGCCCAGGT GCAGCTGCAG GAGTCTGGAG GAGGCTTGGT GCAGCCTGGG GGGTCTCTGA GACTCTCCTG TGCAGCCTCT GGGTTCACCT TCCGTAGATC TATTCTGAAA TGGCTCCGAC AGGCTCCAGG GAAGGAACTG GAGTGGGTGT CCACTATAGA TACATACTCT AATAACACAT ACTATGAAGA CTCCTTGAAG GGCCGATTCA CCATCTCCGC AGACAACGCC AAGAACACGC TGTATCTGCA AATGGACAGC CTGAAACCTG AGGACACGGC CGTGTATTAC TGTGCAAAGG GTGGAGGTGG TATCTACTCC CGCACGTATG ACTACCGGGG CCAGGGGACC CAGGTCACCG TCTCCTCAGC GGCCGCATAC CCGTACGACG TTCCGGACTA CGGTTCCCAC CACCATCACC ATCACTAGAC TGTTGAAAGT TGTTTAGCAA AACCTCATAC AGAAAATTCA TTTACTAACG TCTGGAAAGA CGACAAAACT TTAGATCGTT ACGCTAACTA TGAGGGCTGT CTGTGGAATG CTACAGGCGT TGTCGTTTGT ACTGGTGACG AAACTCAGTG TTACGGTACA TGGGTTCCTA TTGGGCTTGC TATCCCTGAA AATGAGGGTG GTGGCTCTGA GGGTGGCGGT TCTGAGGGTG GCGGTTCTGA GGGTGGCGGT ACTAAACCTC CTGAGTACGG TGATACACCT ATTCCGGGCT ATACTTATAT CAACCCTCTC GACGGCACTT ATCCGCCTGG TACTGAGCAA AACCCCGCTA ATCCTAATCC CTTCTCTTGA GGAGTCTCAG CCTCTTATAC TTTCATGTTT CAGATATAGG TTCCGAAATA GGCAGGTGCA TTAACTGTTA TACGGGCACT GTACTCATGC ACTGACCCCG TTAAACTTAT TACCAGTACA CTCCTGTATC ATCAAAAGCC ATGTATGA
2
30
DNA
Artificial Sequence
2
GACACGAATTCGCCACCATGTTCAGCCACC
3
35
DNA
Artificial Sequence
3
GTGTCAAGCTTTCACTTGTCATCATCATCCTTGTA
4
4541
DNA
Unknown
4
GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTT GCATGCAAAT TCTATTTCAA GGAGACAGTC ATAATGAAAT ACCTATTGCC TACGGCAGCC GCTGGATTGT TATTACTCGC GGCCCAGCCG GCCATGGCCC AGGTGCAGCT GCAGGAGTCT AGAGGGGACC CAGGTCACCG TCTCCTCAGC GGCCGCATAC CCGTACGACG TTCCGGACTA CGGTTCCCAC CACCATCACC ATCACTAGAC TGTTGAAAGT TGTTTAGCAA AACCTCATAC AGAAAATTCA TTTACTAACG TCTGGAAAGA CGACAAAACT TTAGATCGTT ACGCTAACTA TGAGGGCTGT CTGTGGAATG CTACAGGCGT TGTGGTTTGT ACTGGTGACG AAACTCAGTG TTACGGTACA TGGGTTCCTA TTGGGCTTGC TATCCCTGAA AATGAGGGTG GTGGCTCTGA GGTGGCGGT TCTGAGGGTG GCGGTTCTGA GGGTGGCGGT ACTAAACCTC CTGAGTACGG TGATACACCT ATTCCGGGCT ATACTTATAT CAACCCTCTC GACGGCACTT ATCCGCCTGG TACTGAGCAA AACCCCGCTA ATCCTAATCC TTCTCTTGAG GAGTCTCAGC CTCTTAATAC TTTCATGTTT CAGAATAATA GGTTCCGAAA TAGGCAGGGT GCATTAACTG TTTATACGGG CACTGTTACT CAAGGCACTG ACCCCGTTAA AACTTATTAC CAGTACACTC CTGTATCATC AAAAGCCATG TATGACGCTT ACTGGAACGG TAAATTCAGA GACTGCGCTT TCCATTCTGG CTTTAATGAG GACCCATTCG TTTGTGAATA TCAAGGCCAA TCGTCTGACC TGCCTCAACC TCCTGTCAAT GCTGGCGGCG GCTCTGGTGG TGGTTCTGGT GGCGGCTCTG AGGGTGGCGG CTCTGAGGGT GGCGGTTCTG AGGGTGGCGG CTCTGAGGGT GGCGGTTCCG GTGGCGGCTC CGGTTCCGGT GATTTTGATT ATGAAAAAAT GGCAAACGCT AATAAGGGGG CTATGACCGA AAATGCCGAT GAAAACGCGC TACAGTCTGA CGCTAAAGGC AAACTTGATT CTGTCGCTAC TGATTACGGT GCTGCTATCG ATGGTTTCAT TGGTGACGTT TCCGGCCTTG CTAATGGTAA TGGTGCTACT GGTGATTTTG CTGGCTCTAA TTCCCAAATG GCTCAAGTCG GTGACGGTGA TAATTCACCT TTAATGAATA ATTTCCGTCA ATATTTACCT TCTTTGCCTC AGTCGGTTGA ATGTCGCCCT TATGTCTTTG GCGCTGGTAA ACCATATGAA TTTTCTATTG ATTGTGACAA AATAAACTTA TTCCGTGGTG TCTTTGCGTT TCTTTTATAT GTTGCCACCT TTATGTATGT ATTTTCGACG TTTGCTAACA TACTGCGTAA TAAGGAGTCT TAATAAGAAT TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGCCC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT ACTATGGTTG CTTTGACGGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGAA