公开(公告)号：US5763215A

公开(公告)日：1998-06-09

申请号：US400544

申请日：1995-03-08

申请人： Shmaryahu Blumberg ,  Daniella Ben-Meir

发明人： Shmaryahu Blumberg ,  Daniella Ben-Meir

IPC分类号： C07K14/61 ,  C12P21/02 ,  C12N9/48 ,  C12N15/01 ,  C12N15/09

CPC分类号： C07K14/61

摘要： A method of sequentially removing one or more N-terminal amino acid residues from an analog of a eucaryotic polypeptide synthesized in a foreign host, comprises contacting the eucaryotic polypeptide analog with aminopeptidase under suitable conditions permitting sequential removal of N-terminal amino acid residues, where the polypeptide analog contains an amino acid residue or sequence of residues which blocks the action of an aminopeptidase located at a position other than the N-terminal end of the polypeptide analog. A specific embodiment of the invention concerns removing both an N-terminal methionine residue and its adjacent Leucine residue from a growth hormone analog produced in bacteria by contacting the growth hormone analog with Aeromonas aminopeptidase under suitable conditions permitting removal of the N-terminal methionine and Leucine residues. The invention also concerns eucaryotic polypeptide analogs produced in accordance with the methods of the invention.

摘要翻译： 从外来宿主合成的真核多肽的类似物中顺序地除去一个或多个N-末端氨基酸残基的方法包括在合适的条件下使真核多肽类似物与氨基肽酶接触，允许顺序除去N-末端氨基酸残基，其中 多肽类似物包含阻断位于多肽类似物的N-末端以外的位置的氨基肽酶的作用的氨基酸残基或残基序列。 本发明的具体实施方案涉及通过在合适的条件下使生长激素类似物与气单胞菌氨基肽酶接触，从而从细菌中产生的生长激素类似物中除去N-末端甲硫氨酸残基及其相邻的亮氨酸残基，从而允许除去N-末端甲硫氨酸和亮氨酸 残留物。 本发明还涉及根据本发明方法生产的真核生物多肽类似物。