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1.发明申请METHOD OF EVALUATING SKIN BARRIER FUNCTION, METHOD OF SCREENING MATERIAL FOR ENHANCING SKIN BARRIER FUNCTION USING THE EVALUATING METHOD, THE MATERIAL FOR ENHANCING SKIN BARRIER FUNCTION, AND COSMETIC CONTAINING THE MATERIAL FOR ENHANCING SKIN BARRIER FUNCTION 审中-公开评估皮肤障碍功能的方法，使用评估方法，用于增强皮肤屏障功能的材料和包含用于增强皮肤屏障功能的材料的化妆品来增强皮肤屏障功能的材料筛选方法公开(公告)号：US20100068309A1
公开(公告)日：2010-03-18
申请号：US12516483
申请日：2007-12-07
申请人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
发明人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
CPC分类号： G01N33/5044 , G01N33/84
摘要： A method for developing an excellent material for enhancing skin barrier function, and providing a cosmetic which is developed based on the method and has excellent ability to enhance skin barrier function is described. The method evaluates skin barrier function by the steps of 1) culturing normal human epidermal cells in wells having a semipermeable membrane with two sides, in presence and absence of a test substance; 2) adding a medium not containing metal ions to a first side of the semipermeable membrane in each well and a medium containing the metal ions to a second side of the semipermeable membrane in each well, provided that the cells are adhered to the first side of the semipermeable membrane; 3) culturing the cells; 4) measuring the concentration of the metal ions in each medium; and 5) calculating permeability of the metal ions in the epidermal cell layer based on the measured concentrations of the metal ions in each media. The test substance is effective for enhancing skin barrier function when the permeability is reduced in the medium in which the test substance is added compared to the permeability in the medium in which no test substance is added. The larger the level of reduced permeability, the higher the suitability of the test substance as a material for enhancing skin barrier function.
摘要翻译： 描述了开发用于增强皮肤屏障功能的优异材料的方法，并且提供了基于该方法开发并具有优异的增强皮肤屏障功能的能力的化妆品。 该方法通过以下步骤评估皮肤屏障功能：1）在存在和不存在测试物质的情况下，在具有双侧半透膜的孔中培养正常人表皮细胞; 2）在每个孔中将不含金属离子的介质加入到每个孔中的半透膜的第一侧和含有金属离子的介质到每个孔中的半透膜的第二侧，条件是细胞粘附到 半透膜; 3）培养细胞; 4）测量各介质中金属离子的浓度; 以及5）基于每个介质中测量的金属离子浓度来计算表皮细胞层中金属离子的渗透性。 当与添加试验物质的培养基中的渗透性相比，在加入被检物质的培养基中，渗透性降低时，检测物质对增强皮肤屏障功能是有效的。 渗透性降低程度越大，测试物质作为增强皮肤屏障功能的材料的适用性就越高。
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公开(公告)号：US08535904B2
公开(公告)日：2013-09-17
申请号：US13476868
申请日：2012-05-21
申请人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
发明人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
CPC分类号： G01N33/5044 , G01N33/84
摘要： A method for adjusting calcium ion in epidermis is described. The method involves applying a material for enhancing skin barrier function to the skin of a subject. Appropriate materials include Pimpinella extract, Asteroidea extract, L-Carnitine, Royal jelly hydrolysate, Pellicer, Palmaria extract, ε, γ-Glutamyl lysine, Coptis japonica extract and citrus aurantium peel extract
摘要翻译： 描述了调节表皮中钙离子的方法。 该方法包括将用于增强皮肤屏障功能的材料应用于受试者的皮肤。 合适的材料包括皮刺提取物，Asteroidea提取物，L-肉毒碱，蜂王浆水解物，Pellicer，Palmaria提取物，ε，γ-谷氨酰赖氨酸，黄连提取物和柑橘黄皮提取物
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公开(公告)号：US20120282357A1
公开(公告)日：2012-11-08
申请号：US13476868
申请日：2012-05-21
申请人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
发明人： Shohei Kuroda , Takuya Yamamoto , Yuko Saeki , Masumi Kurasawa
CPC分类号： G01N33/5044 , G01N33/84
摘要： A method for adjusting calcium ion in epidermis is described. The method involves applying a material for enhancing skin barrier function to the skin of a subject. Appropriate materials include Pimpinella extract, Asteroidea extract, L-Carnitine, Royal jelly hydrolysate, Pellicer, Palmaria extract, ε, γ-Glutamyl lysine, Coptis japonica extract and citrus aurantium peel extract
摘要翻译： 描述了调节表皮中钙离子的方法。 该方法包括将用于增强皮肤屏障功能的材料应用于受试者的皮肤。 合适的材料包括皮刺提取物，星果苷提取物，L-肉毒碱，蜂王浆水解物，Pellicer，Palmaria提取物，γ-谷氨酰赖氨酸，黄连提取物和柑橘黄皮提取物
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