公开(公告)号：US20200284796A1

公开(公告)日：2020-09-10

申请号：US16807567

申请日：2020-03-03

Applicant: Showa University

Inventor： Michio NAOE ,  Yoshio OGAWA ,  Hikaru ISHII

IPC: G01N33/574 ,  B01L3/00 ,  G01N15/14

Abstract: A detection method of detecting CTCs as target cells contained in a test sample, the method includes a concentration step of concentrating the target cells contained in the test sample, a labeling step of labeling the target cells and other components by bringing the concentrated test sample into contact with first labeling antibodies in which antibodies specifically binding to antigens, which exclude antigens expressing in epithelial cells and specifically express in the target cells, are labeled by a first labeling substance, and into contact with second labeling antibodies in which antibodies binding to antigens expressing in the other components excluding the target cells are labeled by a second labeling substance, and a detection step of detecting, from the test sample, cells that are labeled by the first labeling antibodies and are not labeled by the second labeling antibodies as the target cells.