公开(公告)号：US20120316117A1

公开(公告)日：2012-12-13

申请号：US13572652

申请日：2012-08-12

申请人： Shu-Mei Liang ,  Chun-Yung Huang ,  Chi-Ming Liang

发明人： Shu-Mei Liang ,  Chun-Yung Huang ,  Chi-Ming Liang

IPC分类号： A61K38/38 ,  A61P35/00

CPC分类号： A61K31/7088 ,  A61K38/162 ,  A61K38/38 ,  A61K38/39 ,  A61K39/39 ,  A61K2039/55516

摘要： A method for inhibiting survival of cancer cells in a subject is disclosed. The method comprises administering to a subject in need thereof a therapeutically effective amount of fibrillar albumin. The preparation of the fibrillar albumin comprises: (i) forming a solution comprising an isolated and/or purified globular albumin; (ii) adding a detergent to the solution containing the isolated and/or purified globular albumin, wherein the detergent is one selected from the group consisting of sodium dodecyl sulfate (SDS) and n-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; (iii) applying the solution to a molecular sizing column with a pore size that permits separation of a protein with a molecular weight of at least about 70 kDa so as to promote column-induced formation of the fibrillar albumin from the isolated and/or purified globular albumin; and (iv) eluting the fibrillar albumin from the column, wherein the eluted albumin has a fibrillar structure.

摘要翻译： 公开了抑制受试者中癌细胞存活的方法。 该方法包括向有需要的受试者施用治疗有效量的原纤维白蛋白。 纤维状白蛋白的制备包括：（i）形成包含分离和/或纯化的球状白蛋白的溶液; （ii）向含有分离和/或纯化的球状白蛋白的溶液中加入洗涤剂，其中洗涤剂选自十二烷基硫酸钠（SDS）和正十四烷基-N，N-二甲基-3-氨基 -1-丙磺酸盐; （iii）将溶液施加到具有允许分离至少约70kDa的分子量的蛋白质的孔径的分子筛分柱，以便促进来自分离和/或纯化的柱状诱导的纤维状白蛋白的形成 球状白蛋白 和（iv）从柱中洗脱纤维状白蛋白，其中洗脱的白蛋白具有纤维状结构。

公开(公告)号：US08268974B2

公开(公告)日：2012-09-18

申请号：US12082634

申请日：2008-04-11

申请人： Shu-Mei Liang ,  Chun-Yung Huang ,  Yen-Po Chen ,  Chi-Ming Liang

发明人： Shu-Mei Liang ,  Chun-Yung Huang ,  Yen-Po Chen ,  Chi-Ming Liang

IPC分类号： A61K38/00 ,  A61K45/00 ,  C07K1/00 ,  C07K14/00

CPC分类号： A61K31/7088 ,  A61K38/162 ,  A61K38/38 ,  A61K38/39 ,  A61K39/39 ,  A61K2039/55516

摘要： A method for changing a globular protein structure into a fibrillar protein structure. The method comprising the steps of providing a globular protein, forming a solution containing the globular protein, adding a detergent to the solution containing the globular protein, applying the solution to a molecular sizing column with a pore size of at least 70 kDa and eluting with a solution containing detergent. A method for changing an unfolded protein structure into a fibrillar protein structure. The method comprising the steps of providing a globular protein, forming a solution containing the globular protein, adding a urea to the solution to unfold the globular protein, applying the solution to a molecular sizing column and eluting with a solution containing detergent. A method for treating cancer comprising the steps of providing a protein, changing the protein into a fibrillar structure, and administering a therapeutically effective amount of the fibrillar structure protein to a patient in need thereof. A method for producing a vaccine adjuvant or antigen adjuvant comprising the steps of providing a protein, and changing the protein into a fibrillar structure.

摘要翻译： 一种将球状蛋白结构变为纤维状蛋白结构的方法。 该方法包括以下步骤：提供球状蛋白质，形成含有球状蛋白质的溶液，向含有球状蛋白质的溶液中加入洗涤剂，将溶液施加到孔径至少为70kDa的分子筛分柱上，并用 含有洗涤剂的溶液。 将展开的蛋白质结构改变成纤维蛋白结构的方法。 该方法包括以下步骤：提供球状蛋白质，形成含有球状蛋白质的溶液，向溶液中加入尿素以展开球状蛋白质，将溶液施加到分子筛分柱上并用含有洗涤剂的溶液洗脱。 一种治疗癌症的方法，包括提供蛋白质，将蛋白质改变成纤维结构，以及向有需要的患者施用治疗有效量的纤维结构蛋白的步骤。 一种用于生产疫苗佐剂或抗原佐剂的方法，包括提供蛋白质并将蛋白质变成纤维结构的步骤。

公开(公告)号：US08685923B2

公开(公告)日：2014-04-01

申请号：US13572652

申请日：2012-08-12

申请人： Shu-Mei Liang ,  Chun-Yung Huang ,  Chi-Ming Liang

发明人： Shu-Mei Liang ,  Chun-Yung Huang ,  Chi-Ming Liang

IPC分类号： A61K38/38 ,  C07K14/76 ,  C07K1/16

CPC分类号： A61K31/7088 ,  A61K38/162 ,  A61K38/38 ,  A61K38/39 ,  A61K39/39 ,  A61K2039/55516

摘要： A method for inhibiting survival of cancer cells in a subject is disclosed. The method comprises administering to a subject in need thereof a therapeutically effective amount of fibrillar albumin. The preparation of the fibrillar albumin comprises: (i) forming a solution comprising an isolated and/or purified globular albumin; (ii) adding a detergent to the solution containing the isolated and/or purified globular albumin, wherein the detergent is one selected from the group consisting of sodium dodecyl sulfate (SDS) and n-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; (iii) applying the solution to a molecular sizing column with a pore size that permits separation of a protein with a molecular weight of at least about 70 kDa so as to promote column-induced formation of the fibrillar albumin from the isolated and/or purified globular albumin; and (iv) eluting the fibrillar albumin from the column, wherein the eluted albumin has a fibrillar structure.

摘要翻译： 公开了抑制受试者中癌细胞存活的方法。 该方法包括向有需要的受试者施用治疗有效量的原纤维白蛋白。 纤维状白蛋白的制备包括：（i）形成包含分离和/或纯化的球状白蛋白的溶液; （ii）向含有分离和/或纯化的球状白蛋白的溶液中加入洗涤剂，其中洗涤剂选自十二烷基硫酸钠（SDS）和正十四烷基-N，N-二甲基-3-氨基 -1-丙磺酸盐; （iii）将溶液施加到具有允许分离至少约70kDa的分子量的蛋白质的孔径的分子筛分柱，以便促进来自分离和/或纯化的柱状诱导的纤维状白蛋白的形成 球状白蛋白 和（iv）从柱中洗脱纤维状白蛋白，其中洗脱的白蛋白具有纤维状结构。

公开(公告)号：US20080300186A1

公开(公告)日：2008-12-04

申请号：US12082634

申请日：2008-04-11

申请人： Shu-Mei Liang ,  Chun-Yung Huang ,  Yen-Po Chen ,  Chi-Ming Liang

发明人： Shu-Mei Liang ,  Chun-Yung Huang ,  Yen-Po Chen ,  Chi-Ming Liang

IPC分类号： A61K38/00 ,  C07K1/00 ,  A61P37/00

CPC分类号： A61K31/7088 ,  A61K38/162 ,  A61K38/38 ,  A61K38/39 ,  A61K39/39 ,  A61K2039/55516

摘要： A method for changing a globular protein structure into a fibrillar protein structure. The method comprising the steps of providing a globular protein, forming a solution containing the globular protein, adding a detergent to the solution containing the globular protein, applying the solution to a molecular sizing column with a pore size of at least 70 kDa and eluting with a solution containing detergent. A method for changing an unfolded protein structure into a fibrillar protein structure. The method comprising the steps of providing a globular protein, forming a solution containing the globular protein, adding a urea to the solution to unfold the globular protein, applying the solution to a molecular sizing column and eluting with a solution containing detergent. A method for treating cancer comprising the steps of providing a protein, changing the protein into a fibrillar structure, and administering a therapeutically effective amount of the fibrillar structure protein to a patient in need thereof. A method for producing a vaccine adjuvant or antigen adjuvant comprising the steps of providing a protein, and changing the protein into a fibrillar structure.

摘要翻译： 一种将球状蛋白结构变为纤维状蛋白结构的方法。 该方法包括以下步骤：提供球状蛋白质，形成含有球状蛋白质的溶液，向含有球状蛋白质的溶液中加入洗涤剂，将溶液施加到孔径至少为70kDa的分子筛分柱上，并用 含有洗涤剂的溶液。 将展开的蛋白质结构改变成纤维蛋白结构的方法。 该方法包括以下步骤：提供球状蛋白质，形成含有球状蛋白质的溶液，向溶液中加入尿素以展开球状蛋白质，将溶液施加到分子筛分柱上并用含有洗涤剂的溶液洗脱。 一种治疗癌症的方法，包括提供蛋白质，将蛋白质改变成纤维结构，以及向有需要的患者施用治疗有效量的纤维结构蛋白的步骤。 一种用于生产疫苗佐剂或抗原佐剂的方法，包括提供蛋白质并将蛋白质变成纤维结构的步骤。

公开(公告)号：US20070078028A1

公开(公告)日：2007-04-05

申请号：US11239237

申请日：2005-09-30

申请人： Chun-Yung Huang

发明人： Chun-Yung Huang

IPC分类号： A63B53/00

CPC分类号： A63B53/0466 ,  A63B53/047 ,  A63B2053/0416 ,  A63B2053/042 ,  A63B2053/0433 ,  A63B2053/0437 ,  A63B2209/00

摘要： A golf club head includes a rust-resistant coating layer forming on a base surface of at least one club head component of the golf club head. The rust-resistant coating layer is made from a Fe—Co—Ni alloy including iron of 5-20 wt %, cobalt of 5-25 wt % and nickel of 60-90 wt %, which is a nanometer-scaled material. The rust-resistant coating layer isolates the base surface of the club head component of the golf club head such that oxygen in the atmosphere cannot oxidize the base surface of the base surface of the club head component of the golf club head. Thereby, the rust-resistant coating layer carries out an increase of the antirust ability and hardness of the club head component of the golf club head.

摘要翻译： 高尔夫球杆头包括在高尔夫球杆头的至少一个球杆头部件的基面上形成的防锈涂层。 防锈涂层由包含5-20重量％的铁，5-25重量％的钴和60-90重量％的镍的Fe-Co-Ni合金制成，其为纳米级材料。 防锈涂层隔离高尔夫球杆头的球杆头部件的基面，使得大气中的氧气不能氧化高尔夫球杆头的球杆头部件的基面的基面。 由此，防锈涂层能够提高高尔夫球杆头的球杆头部件的防锈能力和硬度。

公开(公告)号：US20050261081A1

公开(公告)日：2005-11-24

申请号：US10849514

申请日：2004-05-20

申请人： Chan-Tung Chen ,  Chun-Yung Huang

发明人： Chan-Tung Chen ,  Chun-Yung Huang

IPC分类号： A63B53/04

CPC分类号： A63B53/047 ,  A63B53/0466 ,  A63B2053/0416

摘要： A golf club head includes a casing with a front open end to which a striking plate is attached through a layer of brazing material that is formed between a rear surface of the striking plate and a frontmost end face of the casing. One of the striking plate and the front open end of the casing has an extension part which projects outward in a direction away from the opening. The extension part forms a shoulder with one of the first and second outer peripheral faces. During a brazing process, the brazing material is positioned against the shoulder externally of a clearance between the rear surface and the frontmost end face and is then melted to flow into the clearance through a capillary action.

摘要翻译： 高尔夫球杆头包括具有前开口端的壳体，撞击板通过形成在打击板的后表面和壳体的最前端面之间的钎焊材料层附接到该外壳。 壳体的打击板和前开口端之一具有沿远离开口的方向向外突出的延伸部分。 延伸部分形成具有第一和第二外周面之一的肩部。 在钎焊过程中，钎焊材料在后表面和最前端面之间的间隙的外侧抵靠肩部定位，然后通过毛细管作用熔化以流入间隙。

公开(公告)号：US20050227781A1

公开(公告)日：2005-10-13

申请号：US11150177

申请日：2005-06-13

申请人： Chun-Yung Huang ,  Chan-Tung Chen

发明人： Chun-Yung Huang ,  Chan-Tung Chen

IPC分类号： A63B53/04 ,  A63B53/06

CPC分类号： A63B53/04 ,  A63B53/047 ,  A63B53/06 ,  A63B60/00 ,  A63B2053/0433 ,  A63B2209/00

摘要： A weight member for a golf club head is made of a WMoNi alloy by powder metallurgy or a precision casting process. The WMoNi alloy includes tungsten 1-35 wt %, molybdenum 4-55 wt %, and nickel 25-95 wt %, and has a specific gravity ranging between 8.6 and 13.3. Molybdenum increases the density of the weight member and improves the rust-resisting property of the weight member. The tungsten, molybdenum, and nickel provide a uniform metallographic phase. Uniformity of polishing of the weight member is thus improved.

摘要翻译： 用于高尔夫球杆头的重量件通过粉末冶金或精密铸造工艺由WMoNi合金制成。 WMoNi合金包括1-35重量％的钨，4-55重量％的钼和25-95重量％的镍，其比重在8.6和13.3之间。 钼增加了重量部件的密度，提高了重量部件的防锈性。 钨，钼和镍提供均匀的金相。 因此，重量构件的抛光均匀性得到改善。

公开(公告)号：US06852041B1

公开(公告)日：2005-02-08

申请号：US10624526

申请日：2003-07-23

申请人： Chun-Yung Huang

发明人： Chun-Yung Huang

IPC分类号： A63B53/04

CPC分类号： A63B53/047 ,  A63B53/0466 ,  A63B2053/0416 ,  A63B2209/00 ,  Y10T29/49885 ,  Y10T29/49908 ,  Y10T29/49915 ,  Y10T29/49922

摘要： A golf club head includes a golf club head body and a striking plate. The golf club head body has a recession in a side thereof. The striking plate is embedded in the recession of the golf club head body. The striking plate includes a front face acting as a striking face and a rear face that is a highly polished surface. A reinforcing metal layer is formed on the highly polished surface of the striking plate and has a hardness higher than 1000 HV for protecting the highly polished surface.

公开(公告)号：US20050020376A1

公开(公告)日：2005-01-27

申请号：US10624526

申请日：2003-07-23

申请人： Chun-Yung Huang

发明人： Chun-Yung Huang

IPC分类号： A63B53/04

CPC分类号： A63B53/047 ,  A63B53/0466 ,  A63B2053/0416 ,  A63B2209/00 ,  Y10T29/49885 ,  Y10T29/49908 ,  Y10T29/49915 ,  Y10T29/49922

摘要： A golf club head includes a golf club head body and a striking plate. The golf club head body has a recession in a side thereof. The striking plate is embedded in the recession of the golf club head body. The striking plate includes a front face acting as a striking face and a rear face that is a highly polished surface. A reinforcing metal layer is formed on the highly polished surface of the striking plate and has a hardness higher than 1000 HV for protecting the highly polished surface.

摘要翻译： 高尔夫球杆头包括高尔夫球杆头本体和击打板。 高尔夫球杆头体在其一侧具有凹陷。 打击板嵌入高尔夫球杆头本体的凹陷中。 打击板包括作为打击面的前表面和作为高度抛光表面的后表面。 在打击板的高度抛光的表面上形成增强金属层，并且具有高于1000HV的硬度以保护高度抛光的表面。

公开(公告)号：US08348785B2

公开(公告)日：2013-01-08

申请号：US12401316

申请日：2009-03-10

申请人： Chan-Tung Chen ,  Chun-Yung Huang ,  Ching-Fen Lee ,  Chun-Wei Chen

发明人： Chan-Tung Chen ,  Chun-Yung Huang ,  Ching-Fen Lee ,  Chun-Wei Chen

IPC分类号： A63B53/04

CPC分类号： A63B53/047 ,  A63B2053/0408 ,  A63B2053/0416 ,  A63B2053/042 ,  A63B2053/0433 ,  A63B2209/00

摘要： Disclosed is a golf club head having a striking plate made of a high-strength aluminum alloy including at most 0.06 wt % of Si, at most 0.1 wt % of Fe, 2 to 2.5 wt % of Cu, 1.5 to 2.5 wt % of Mg, 5 to 9.5 wt % of Zn, at most 0.05 wt % of Mn, 0.1 to 0.2 wt % of Zr, at most 0.05 wt % of Cr, at most 0.05 wt % of Ti, 0.01 to 0.1 wt % of Sc, and balance Al, wherein the weight ratio of Zn over Mg is from about 3.2 to about 4.4.

摘要翻译： 公开了一种具有由高强度铝合金制成的击打板的高尔夫球杆头，其包括至多0.06重量％的Si，至多0.1重量％的Fe，2至2.5重量％的Cu，1.5至2.5重量％的Mg ，Zn：5〜9.5wt％，Mn：0.05wt％，Zr：0.1〜0.2wt％，Cr：0.05wt％以下，Ti：0.05wt％以下，Sc：0.01〜0.1wt％ 余量为Al，其中Zn与Mg的重量比为约3.2至约4.4。