公开(公告)号：US6114139A

公开(公告)日：2000-09-05

申请号：US513974

申请日：1995-09-14

申请人： Shuji Hinuma ,  Masaki Hosoya ,  Ryo Fujii ,  Tetsuya Ohtaki ,  Shoji Fukusumi ,  Kazuhiro Ohgi

发明人： Shuji Hinuma ,  Masaki Hosoya ,  Ryo Fujii ,  Tetsuya Ohtaki ,  Shoji Fukusumi ,  Kazuhiro Ohgi

IPC分类号： C07K14/705 ,  C07K14/72 ,  C12N15/12 ,  C12Q1/68 ,  C12N5/10 ,  C12N15/63

CPC分类号： C12Q1/6876 ,  C07K14/705 ,  C07K14/70571 ,  C07K14/723 ,  C12Q2600/158

摘要： DNA primers effective in screening G protein coupled receptor protein-encoding DNA fragments are provided. The primers which are complementary to nucleotide sequences that are in community with (homologous to) the nucleotide sequences encoding amino acid sequences corresponding to or near the first membrane-spanning domain or the sixth membrane-spanning domain each of known various G protein coupled receptor proteins were designed and synthesized. Methods of amplifying G protein coupled receptor protein-encoding DNAs using the above DNA primers, and novel target G protein coupled receptor protein-encoding DNAs are also provided. Screening of DNA libraries can be efficiently carried out. Human pituitary gland or amygdala-derived and mouse pancreas-derived G protein coupled receptor proteins, etc. or salts thereof, partial peptides thereof, DNAs coding for the above G protein coupled receptor proteins, processes for producing the above G protein coupled receptor proteins, methods of determining ligands for the above G protein coupled receptor proteins, methods of screening compounds that inhibit the binding between the ligand and the G protein coupled receptor proteins or screening kits therefor, compounds or salts thereof obtained by the above screening method or the screening kit, pharmaceutical compositions containing the above compounds or salts thereof, and antibodies against the above protein coupled receptor proteins or partial peptides thereof are provided.

摘要翻译： PCT No.PCT / JP95 / 01599 Sec。 371 1995年9月14日第 102（e）1995年9月14日PCT PCT 1995年8月10日PCT公布。 出版物WO96 / 05302 日期1996年2月22日提供了有效筛选编码G蛋白偶联受体蛋白的DNA片段的DNA引物。 与与编码对应于或接近第一跨膜结构域或第六跨膜结构域的氨基酸序列的核苷酸序列（同源的）的核苷酸序列互补的引物，每个已知的各种G蛋白偶联受体蛋白 被设计和合成。 还提供了使用上述DNA引物扩增G蛋白偶联受体蛋白质编码DNA的方法，以及新的靶向G蛋白偶联受体蛋白质编码DNA。 可以有效地进行DNA文库的筛选。 人类垂体腺或来自扁桃体的小鼠胰腺衍生的G蛋白偶联受体蛋白等，或其盐，其部分肽，编码上述G蛋白偶联受体蛋白质的DNA，用于产生上述G蛋白偶联受体蛋白质的方法， 确定上述G蛋白偶联受体蛋白质的配体的方法，筛选抑制配体和G蛋白偶联受体蛋白质或其筛选试剂盒之间的结合的化合物的方法，通过上述筛选方法获得的化合物或其盐或筛选试剂盒 提供含有上述化合物或其盐的药物组合物和针对上述蛋白质偶联受体蛋白质或其部分肽的抗体。

公开(公告)号：US06399325B1

公开(公告)日：2002-06-04

申请号：US08540650

申请日：1995-10-11

申请人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Tetsuya Ohtaki ,  Masaki Hosoya ,  Kazuhiro Ohgi ,  Haruo Onda

发明人： Shuji Hinuma ,  Ryo Fujii ,  Shoji Fukusumi ,  Tetsuya Ohtaki ,  Masaki Hosoya ,  Kazuhiro Ohgi ,  Haruo Onda

IPC分类号： C12N1512

CPC分类号： C07K14/723

摘要： Galanin receptor proteins, production and use thereof including screening of galanin receptor agonists and antagonists are provided. Galanin receptor proteins, etc. or salts thereof, partial peptides thereof, DNAs coding for the above galanin receptor protein, processes for producing the above receptor protein, methods of screening for a galanin receptor agonist and/or antagonist or screening kits therefor, agonist and/or antagonist compounds or salts thereof obtained by the above screening method or the screening kit, pharmaceutical compositions containing the above compound or its salt, and antibodies against the above receptor protein are provided. It is allowable to efficiently screen a galanin receptor agonist or antagonist by using the galanin receptor protein, the partial peptide thereof, the galanin receptor protein-encoding DNA, the receptor protein-containing cell or its membrane fraction. The pharmaceuticals thus screened or characterized permits various applications including prophylactic and/or therapeutic treatments against a variety of diseases, e.g., stomach ulcer, diabetes, Alzheimer's disease, dementia, etc. and a sedative.

摘要翻译： 提供了Galanin受体蛋白质，其生产和使用，包括甘露糖素受体激动剂和拮抗剂的筛选。 加兰林素受体蛋白等或其盐，其部分肽，编码上述甘丙肽受体蛋白质的DNA，产生上述受体蛋白质的方法，筛选甘丙肽受体激动剂和/或拮抗剂的方法或其筛选试剂盒，激动剂和 /或通过上述筛选方法获得的拮抗剂化合物或其盐或筛选试剂盒，含有上述化合物或其盐的药物组合物和抗上述受体蛋白的抗体。 可以通过使用甘丙肽受体蛋白，其部分肽，编码甘露糖素受体蛋白的DNA，含受体蛋白的细胞或其膜部分来有效筛选甘丙肽受体激动剂或拮抗剂。 如此筛选或表征的药物允许各种应用，包括针对各种疾病（例如胃溃疡，糖尿病，阿尔茨海默病，痴呆等）和镇静剂的预防和/或治疗性治疗。