利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

2 条记录 (耗时 0.012 秒)

    THIFENSULFURON HYDROLASE GENE TSME AND USES THEREOF
    1.
    发明申请
    THIFENSULFURON HYDROLASE GENE TSME AND USES THEREOF 有权
    THIFENSULFURON水解酶基因TSME及其用途

    公开(公告)号：US20140325700A1

    公开(公告)日：2014-10-30

    申请号：US14234824

    申请日：2012-07-17

    申请人： Shunpeng Li ,  Jian He ,  Baojian Hang

    发明人： Shunpeng Li ,  Jian He ,  Baojian Hang

    IPC分类号： A62D3/02 ,  C12N9/14

    CPC分类号： A62D3/02 ,  A62D2101/04 ,  A62D2101/26 ,  A62D2101/28 ,  C02F3/342 ,  C02F2101/36 ,  C02F2101/40 ,  C12N9/14 ,  C12N9/18 ,  C12N15/8259 ,  C12N15/8274 ,  C12N15/8278

    摘要： The present invention relates to the field of applied and environmental microorganism and agriculture. Disclosed are a thifensulfuron hydrolase gene tsmE and uses thereof. The thifensulfuron hydrolase gene tsmE has a nucleotide sequence of SEQ ID NO.1, full length of 1194 bp, and G+C content of 51.09%, and encodes 398 amino acids with an amino acid sequence of SEQ ID NO.2. The thifensulfuron hydrolase TsmE provided by the present invention can degrade completely 100 mg/L thifensulfuron within 1 hour into the herbicidally inactive product thiophenesulfonic acid; in addition, the TsmE also degrade completely 100 mg/L haloxyfop-R-methyl within 1 hour. Therefore, the thifensulfuron hydrolase gene tsmE is useful in construction of thifensulfuron-resistant transgenic crops and bioremediation of thifensulfuron or haloxyfop-R-methyl-contaminated environments.

    摘要翻译： 本发明涉及应用环境微生物和农业领域。 披露了噻吩磺隆水解酶基因tsmE及其用途。 噻吩磺隆水解酶基因tsmE具有SEQ ID NO.1的核苷酸序列，全长1194bp，G + C含量为51.09％，编码398个氨基酸，其氨基酸序列为SEQ ID NO.2。 本发明提供的噻吩磺隆水解酶TsmE可在1小时内将完全100mg / L的噻吩磺隆降解为除草活性产物噻吩磺酸; 此外，TsmE在1小时内也完全降解了100 mg / L的卤代甲氧基-N-甲基。 因此，噻吩磺隆水解酶基因tsmE可用于构建噻磺隆抗性转基因作物和噻吩磺隆或环氧磷脂-R-甲基受污染环境的生物修复。