公开(公告)号：US12203831B2

公开(公告)日：2025-01-21

申请号：US17250853

申请日：2019-09-23

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Frank Vitzthum ,  Huey Lee ,  Michelle Son

IPC: A61K9/127 ,  G01N1/34 ,  G01N1/40

Abstract: Disclosed herein are compositions and methods for removing interfering substances from biological samples, biochemical assays, or reagents used in biological samples using porous liposomes that capture and in some instances enrich said interfering substances within the liposomes.

公开(公告)号：US20210302417A1

公开(公告)日：2021-09-30

申请号：US17250536

申请日：2019-09-27

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Frank Vitzthum ,  Paul D'Agostino ,  Kayla Cortese

IPC: G01N33/543 ,  A61K47/68

Abstract: The disclosure provides an in vitro diagnostic method that is sensitive and accurate for detection and quantification of targets of interest that are associated with disease. There is an ongoing need for a highly sensitive detection system. In addition to the methods, stable complexes and kits comprising the components necessary to perform the disclosed detection methods are described.

公开(公告)号：US11226338B2

公开(公告)日：2022-01-18

申请号：US16321709

申请日：2017-08-10

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Frank Vitzthum ,  Herbert Schwarz ,  Anja Haude-Barten

IPC: G01N33/68 ,  G01N33/48

Abstract: Methods for detection and quantitative measurement of proteins with a Coomassie Brilliant Blue Assay with improved sensitivity and maintaining high linearity over a broad measuring range are provided herein. In particular, a method of detecting a protein in a protein-containing sample is provided. The method includes providing a sample including a protein, a reagent including Coomassie Brilliant Blue and having a pH between 0.85 and 1.1, combining the sample and the reagent, and determining absorption at first and second wavelength to determine the amount of protein in the sample. The ratio of the absorption value at the first wavelength between about 580 to 620 nm to the absorption value at the second wavelength between about 520 to 370 nm is used in the spectral photometric determination of the amount of protein.

公开(公告)号：US20210278412A1

公开(公告)日：2021-09-09

申请号：US16321709

申请日：2017-08-10

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Frank Vitzthum ,  Herbert Schwarz ,  Anja Haude-Barten

IPC: G01N33/68

Abstract: The present invention relates to the detection and quantitative measurement of proteins with the Coomassie Brilliant Blue Assay with improved sensitivity and maintaining high linearity over a broad measuring range. In particular the present invention relates to a method of detecting a protein in a protein-containing sample, comprising providing a sample comprising a protein, a reagent comprising Coomassie Brilliant Blue, combining the sample and the reagent and determining of the absorption to determine the amount of protein in the sample, wherein the pH-value of the reagent is between pH-value 0.85 and pH-value 1.1, and the ratio of the absorption value at a first wavelength of between about 580 to 620 nm to the one at a second wavelength of between about 520 to 370 nm is used in the spectral photometric determination of the amount of protein.