公开(公告)号：US20250011845A1

公开(公告)日：2025-01-09

申请号：US18707776

申请日：2022-11-04

Applicant: Solventum Intellectual Properties Company

Inventor： Annabelle Watts ,  Tonya D. Bonilla ,  Jerald K. Rasmussen ,  Joshua M. Fishman ,  Lindsay L. Traeger ,  Semra Colak Atan

IPC: C12Q1/6834 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/686 ,  G01N27/447

Abstract: A method of processing polynucleic acids is described comprising: providing a solid support comprising ligands with an amide group: exposing the solid support to polynucleic acid molecules in a buffer having a pH less than 5.5 to bind at least a portion of the polynucleic acid molecules to the ligands: c) exposing the solid support (e.g. particles) with bound polynucleic acid molecules to a buffer having a pH greater than 6 to release a portion of the bound polynucleic acid molecules from the ligands of the particles and to retain a portion of the polynucleic acid molecules bound to the solid support (e.g. particles); and utilizing the solid support with retained bound polynucleic acid molecules or suspension thereof.