公开(公告)号：US20120190080A1

公开(公告)日：2012-07-26

申请号：US13278100

申请日：2011-10-20

申请人： Soo An SHIN ,  Jin Sook CHANG ,  Hye Won UM ,  Jae Hyun JO ,  Byeong Cheol SONG ,  Kyoung Min LEE

发明人： Soo An SHIN ,  Jin Sook CHANG ,  Hye Won UM ,  Jae Hyun JO ,  Byeong Cheol SONG ,  Kyoung Min LEE

IPC分类号： C12P13/12 ,  C12N1/21 ,  C12N15/63 ,  C12N15/55 ,  C12N9/14

CPC分类号： C12Y205/01065 ,  C12N9/1085 ,  C12P13/12

摘要： Disclosed is an O-phosphoserine sulfhydrylase (OPSS) mutant which has a Mycobacterium smegmatis-derived amino acid sequence corresponding to that of SEQ ID NO: 1 which is devoid of three to seven C-terminal amino acid residues. Also, a nucleic acid molecule encoding the OPSS mutant, an expression vector carrying the nucleic acid molecule, and a transformant transformed with the expression vector are disclosed. In addition, a method is provided for producing cysteine in which O-phospho-L-serine (OPS) is reacted with a sulfide in the presence of the OPSS mutant. The OPSS mutant has improved enzymatic activity and can be applied to the environmentally friendly production of L-cysteine through a simple enzymatic conversion reaction.

摘要翻译： 公开了一种O-磷酸丝氨酸二硫氢化酶（OPSS）突变体，其具有对应于SEQ ID NO：1的分枝杆菌衍生的氨基酸序列，其缺少3至7个C-末端氨基酸残基。 此外，公开了编码OPSS突变体的核酸分子，携带核酸分子的表达载体和用表达载体转化的转化体。 此外，提供了在OPSS突变体存在下，使O-磷酸-L-丝氨酸（OPS）与硫化物反应的半胱氨酸的制造方法。 OPSS突变体具有改善的酶活性，可以通过简单的酶转化反应应用于环保生产L-半胱氨酸。

公开(公告)号：US20120190083A1

公开(公告)日：2012-07-26

申请号：US13278106

申请日：2011-10-20

申请人： Jin Sook CHANG ,  Jae Hyun JO ,  Hyun Ae BAE ,  Byeong Cheol SONG ,  Sol KIM ,  Hye Won KIM ,  Hye Won UM ,  Sung Hoo JHON ,  Yong Uk SHIN ,  Eun Bin YANG ,  Kyoung Min LEE ,  Soo An SHIN

发明人： Jin Sook CHANG ,  Jae Hyun JO ,  Hyun Ae BAE ,  Byeong Cheol SONG ,  Sol KIM ,  Hye Won KIM ,  Hye Won UM ,  Sung Hoo JHON ,  Yong Uk SHIN ,  Eun Bin YANG ,  Kyoung Min LEE ,  Soo An SHIN

IPC分类号： C12P13/12 ,  C12N1/21

CPC分类号： C12P13/12 ,  C12N9/1085 ,  C12N9/16 ,  C12N15/70 ,  C12N15/77 ,  C12P13/06 ,  C12Y205/01065 ,  C12Y301/03003

摘要： The present invention provides methods for the production of cysteine or derivates thereof by culturing a microorganism having reduced activity of endogenous phosphoserine phosphatase and the activity of PhnC, PhnD, and PhnE is reduced, and enhanced activity of phosphoglycerate dehydrogenase and/or phosphoserine aminotransferase. The O-phosphoserine produced by such an organism can then be reacted with a sulfide in the presence of a sulfydrylase or a microorganism expressing a sulfhydrylase to produce cysteine or a derivative thereof. Microorganisms having these reduced and enhanced properties noted above are also provided herein.

摘要翻译： 本发明提供了通过培养具有降低的内源性磷酸丝氨酸磷酸酶活性的微生物来生产半胱氨酸或其衍生物的方法，并且PhnC，PhnD和PhnE的活性降低，并且提高了磷酸甘油酸脱氢酶和/或磷酸丝氨酸氨基转移酶的活性。 由这样的生物体产生的O-磷酸丝氨酸然后可以在硫酸酯或表达巯基化酶的微生物的存在下与硫化物反应，以产生半胱氨酸或其衍生物。 本文还提供了具有上述这些降低和增强性能的微生物。