公开(公告)号：US20230167191A1

公开(公告)日：2023-06-01

申请号：US17920724

申请日：2021-04-23

申请人： Sorrento Therapeutics, Inc.

发明人： Henry Hongjun Ji ,  Runqiang Chen ,  Xiaomei Yuan

IPC分类号： C07K16/28 ,  C07K14/725 ,  C07K14/715 ,  C07K14/73 ,  C07K14/705 ,  A61K48/00 ,  A61K35/17

CPC分类号： C07K16/2896 ,  C07K14/7051 ,  C07K14/7155 ,  C07K14/70514 ,  C07K14/70521 ,  A61K48/005 ,  A61K35/17 ,  C07K2319/00 ,  A61K38/00

摘要： The present disclosure provides transgenic cells that express memory dimeric antigen receptors (mDARs), where the mDAR constructs comprise a JAK-STAT intracellular region having a cytokine receptor intracellular region which includes Box 1 and Box 2 motifs for binding a Janus kinase (JAK) which can play a role in JAK-STAT cellular signaling pathway to induce effector cell activation and proliferation. In one embodiment, the JAK-STAT intracellular region further comprises a CD3zeta intracellular signaling region having an intact ITAM region, or having ITAM 1 and 3, or having only ITAM 3 with a partial deletion. Transgenic cells expressing the mDAR constructs exhibit potent cytotoxicity, and release reduced levels of cytokines, compared to traditional DARs that lack a cytokine receptor intracellular region. The mDAR constructs have antibody-like properties as they bind specifically to a target antigen. Transgenic cells expressing the mDAR constructs can be used for directed cell therapy.