Reagent for detecting non-A, non-B viral hepatitis (NANBH) and an
immunoenzymatic method for detecting NANBH antigens in fecal extracts
    3.
    发明授权
    Reagent for detecting non-A, non-B viral hepatitis (NANBH) and an immunoenzymatic method for detecting NANBH antigens in fecal extracts 失效
    用于检测非A,非B病毒性肝炎(NANBH)的试剂和用于检测粪便提取物中的NANBH抗原的免疫酶法

    公开(公告)号:US4871659A

    公开(公告)日:1989-10-03

    申请号:US35172

    申请日:1987-04-07

    申请人: Jacques Pillot

    发明人: Jacques Pillot

    摘要: Anti-NANBH (anti-non-A, non-B hepatitis) antibody comprises IgM isolated from sera of monkeys artificially infected with extracts of feces from patients known to be suffering from epidemic NANB hepatitis. A process for isolating an antibody of this type is provided. A reagent for detecting epidemic NANB hepatitis comprises the anti-NANBH IgM fixed to a solid support. The reagent is useful for diagnosing epidemic NANB viral hepatitis by forming an immunological complex between the anti-NANBH IgM and antigen in a fecal extract from a patient being diagnosed, and then incubating the immunological complex with enzyme-labeled IgG from serum of a human convalescing from epidemic NANBH. The complex is detected by developing the enzyme.

    摘要翻译: 抗NANBH(抗非A型,非乙型肝炎)抗体包含从已知患有流行性NANB肝炎的患者的粪便提取物人为感染的猴血清分离的IgM。 提供了分离这种抗体的方法。 用于检测流行性NANB肝炎的试剂包括固定在固体支持物上的抗NANBH IgM。 该试剂可用于通过在被诊断的患者的粪便提取物中形成抗NANBH IgM和抗原之间的免疫复合物来诊断流行的NANB病毒性肝炎,然后将免疫复合物与来自人类康复的血清的酶标记的IgG一起温育 来自流行的NANBH。 通过开发酶检测复合物。