公开(公告)号：US07528292B2

公开(公告)日：2009-05-05

申请号：US10523474

申请日：2003-08-01

申请人： Stefan Herz ,  Hans-Ulrich Koop ,  Timothy J. Golds ,  Christian Eibl

发明人： Stefan Herz ,  Hans-Ulrich Koop ,  Timothy J. Golds ,  Christian Eibl

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82

CPC分类号： C12N15/8214

摘要： The invention provides processes for generating transgenic plants or plant cells transformed on their plastome, comprising introducing into plant plastids a first and a second DNA molecule, wherein the first DNA molecule contains a first region homologous to a region of the plastome for directing plastome integration and a first sequence of interest, and the second DNA molecule contains a second region homologous to a region of the plastome and a second sequence of interest, whereby a sequence segment of the first sequence of interest is homologous to a sequence segment of the second sequence of interest, and selecting transformants having an integration sequence stably integrated in the plastome, whereby the integration sequence contains at least a portion of the first and second sequences of interest as a continuous sequence. The invention further provides plant cells, plants, and seeds of plants produced by such processes.

摘要翻译： 本发明提供用于产生在其塑料体上转化的转基因植物或植物细胞的方法，包括向植物质体中引入第一和第二DNA分子，其中所述第一DNA分子包含与所述塑性体的区域同源的第一区域，用于引导塑性体整合， 第一个感兴趣的序列，并且第二个DNA分子包含与Plastome的区域同源的第二个区域和第二个感兴趣的序列，其中第一个目的序列的序列片段与第二个序列的序列片段同源 兴趣，并选择具有稳定整合在塑性体中的整合序列的转化体，由此整合序列包含作为连续序列的第一和第二感兴趣序列的至少一部分。 本发明还提供通过这些方法生产的植物的植物细胞，植物和种子。

公开(公告)号：US07371923B2

公开(公告)日：2008-05-13

申请号：US10482549

申请日：2002-04-30

申请人： Hans-Ulrich Koop ,  Stefan Mühlbauer ,  Sebastian Klaus ,  Christian Eibl ,  Fong-Chin Huang ,  Timothy J. Golds

发明人： Hans-Ulrich Koop ,  Stefan Mühlbauer ,  Sebastian Klaus ,  Christian Eibl ,  Fong-Chin Huang ,  Timothy J. Golds

IPC分类号： C12N15/82

CPC分类号： C12N15/8212 ,  C12N15/821 ,  C12N15/8214 ,  C12N15/8257

摘要： A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.

摘要翻译： 一种产生转化的植物或植物细胞的方法，所述转基因植物或植物细胞在其塑料体上转化并且没有选择标记，包括以下步骤：（a）用DNA转化植物或植物细胞的质体，所述DNA包括：（i）赋予复制的核苷酸序列 所述DNA在植物细胞中，（ii）至少一种感兴趣的序列，（iii）侧翼于所述至少一个感兴趣序列侧翼的序列，所述至少一个感兴趣序列用于将所述至少一个感兴趣的序列稳定整合到质体基因组中，以及（iv ）在所述感兴趣的序列侧翼的所述序列之外的选择标记; （b）允许在存在选择压力的情况下将所述至少一个感兴趣序列整合到塑料瓶中; （c）通过释放选择压力允许所述选择标记序列的丢失; 和（d）回收在其塑料上遗传转化并且没有所述选择标记的细胞和/或植物。