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公开(公告)号:US10167459B2
公开(公告)日:2019-01-01
申请号:US15516372
申请日:2015-10-02
IPC分类号: C12N9/24 , C12N9/26 , A23L27/30 , A23L33/125 , C12P19/16
摘要: The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesizing FOS using the variants is also claimed.
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公开(公告)号:US20170298332A1
公开(公告)日:2017-10-19
申请号:US15516372
申请日:2015-10-02
IPC分类号: C12N9/26 , A23L27/33 , A23L33/125 , C12P19/16
CPC分类号: C12N9/2431 , A23L27/33 , A23L33/125 , A23V2002/00 , C12P19/16 , C12Y302/01026
摘要: The invention provides variants of the Aspergillus japonicus β-fructofuranosidase enzyme which have been modified so as to improve synthesis of inulin-type fructooligosaccharides (FOS) from sucrose. One or more substitutions may be made to the parent β-fructofuranosidase polypeptide at amino acid positions 121, 159, 302 and/or 471 of the mature peptide (SEQ ID NO: 3), corresponding to crystal positions 140, 178, 321 and 490. A method of synthesising FOS using the variants is also claimed.
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