STEVIOL GLYCOSIDE PRODUCTION
    7.
    发明申请

    公开(公告)号:US20170306377A1

    公开(公告)日:2017-10-26

    申请号:US15516854

    申请日:2015-10-08

    IPC分类号: C12P19/56 C12N15/52 A23L27/30

    摘要: The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises:a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.