公开(公告)号：US20250108071A1

公开(公告)日：2025-04-03

申请号：US18978710

申请日：2024-12-12

Applicant: Stemcell Technologies Canada Inc.

Inventor： Jonathan B. GILBERT ,  Bu WANG ,  Scott LOUGHHEAD ,  Howard BERNSTEIN ,  Armon R. SHAREI ,  Finola MOORE

IPC: A61K35/18 ,  A61K35/19 ,  A61K39/00 ,  A61K39/385 ,  A61P37/06 ,  C12M1/12 ,  C12M3/06 ,  C12N5/078 ,  C12N15/85 ,  C12N15/87

Abstract: The present invention provides methods for inducing tolerance and/or suppressing an immune response to an antigen by passing a cell suspension containing an anucleate cell through a constriction, wherein the constriction deforms the cell thereby causing a perturbation of the cell such that an antigen and/or tolerogenic factor enters the cell. In some embodiments, the anucleate cell is delivered to an individual and the antigen is delivered to and processed in a tolerogenic environment to induce tolerance and/or suppress an immune response to the antigen.

公开(公告)号：US20240376415A1

公开(公告)日：2024-11-14

申请号：US18665917

申请日：2024-05-16

Applicant: STEMCELL TECHNOLOGIES CANADA INC.

Inventor： Maisam DADGAR ,  Jacquelyn L. Sikora HANSON ,  Armon R. SHAREI

IPC: C12M3/06 ,  B01L3/00 ,  C12M1/26

Abstract: A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.