公开(公告)号：US07196234B2

公开(公告)日：2007-03-27

申请号：US11478821

申请日：2006-06-30

申请人： Stephan Doswald ,  Steven Paul Hanlon ,  Ernst Kupfer

发明人： Stephan Doswald ,  Steven Paul Hanlon ,  Ernst Kupfer

IPC分类号： C07C29/14

CPC分类号： C12P7/04

摘要： The invention relates to a scalable biocatalytic process for the preparation of S-1,1,1-trifluoro-2-propanol with a enantiomeric excess of >99% by asymmetric microbial reduction of 1,1,1-trifluoroacetone with Baker's yeast.

摘要翻译： 本发明涉及通过用Baker's酵母通过不对称微生物还原1,1,1-三氟丙酮来制备对映体过量> 99％的S-1,1,1-三氟-2-丙醇的可扩展的生物催化方法。

公开(公告)号：US6156911A

公开(公告)日：2000-12-05

申请号：US491557

申请日：2000-01-26

申请人： Stephan Doswald ,  Ernst Kupfer ,  Gerhard Steinbauer ,  Erich Steinwender

发明人： Stephan Doswald ,  Ernst Kupfer ,  Gerhard Steinbauer ,  Erich Steinwender

IPC分类号： C07D305/12

CPC分类号： C07D305/12

摘要： The present invention relates to a method for the purification of the lipstatin. Impurities are removed from a lipstatin preparation by: a) mixing the preparation with a polar phase and to form a first two phase system in which the ratio of the lipstatin to the non-polar impurities in the polar phase is greater than in the preparation, and separating the phases from the first two phase system and collecting the lipstatin-enriched polar phase, and b) i) diluting the lipstatin-enriched polar phase from step a) with water to form a diluted lipstatin-enriched polar phase containing the polar solvent in a percentage of at least seventy percent of the polar phase and lower than in the lipstatin-enriched polar phase from step a), and mixing the diluted lipstatin-enriched polar phase with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in the same or greater relative amount to the polar phase as in the first two phase system; or ii) mixing the lipstatin-enriched polar phase from step a) with a second non-polar phase, the second non-polar phase being the same non-polar solvent as the first non-polar phase in a greater relative amount to the polar phase as in the first two phase system; to form a second two phase system in which the ratio of the lipstatin to the polar impurities in the second non-polar phase is greater than in the lipid-enriched polar phase from step a), and separating the phases from the second two phase system and collecting the lipstatin-enriched second non-polar phase.

摘要翻译： 本发明涉及一种脂质体抑制剂的纯化方法。 通过以下步骤从脂质体抑制剂制剂中除去杂质：a）将制剂与极性相混合并形成第一两相体系，其中极性相中的脂质体抑制剂与非极性杂质的比例大于制剂中的比例， 并分离相与前两相系统并收集富含脂肪抑素的极性相，和b）i）用水稀释步骤a）中富含脂肪抑素的极性相，以形成含有极性溶剂的稀释的富含lipstatin的极性相 以百分之七十的极性相，并低于步骤a）中富含脂肪抑素的极性相，并将稀释的富含lipstatin的极性相与第二非极性相混合，第二非极性相 与第一非极性相相同，非极性溶剂相对于极性相相同或更大的相对于前两相系统; 或ii）将来自步骤a）的富含脂质体抑制素的极性相与第二非极性相混合，所述第二非极性相与第一非极性相相同，与极性相对的量相同为非极性溶剂 相位如前两相系统; 以形成第二两相体系，其中所述第二非极性相中所述脂质体抑制剂与所述极性杂质的比例大于来自步骤a）的富脂极性相中的比例，并且将所述相与所述第二两相系统 并收集富含lipstatin的第二非极性相。

公开(公告)号：US20070009999A1

公开(公告)日：2007-01-11

申请号：US11478821

申请日：2006-06-30

申请人： Stephan Doswald ,  Steven Hanlon ,  Ernst Kupfer

发明人： Stephan Doswald ,  Steven Hanlon ,  Ernst Kupfer

IPC分类号： C12P7/04

CPC分类号： C12P7/04

摘要： The invention relates to a scalable biocatalytic process for the preparation of S-1,1,1,-trifluoro-2-propanol with a enantiomeric excess of >99% by asymmetric microbial reduction of 1,1,1 -trifluoroacetone with Baker's yeast.

摘要翻译： 本发明涉及通过用Baker's酵母不对称的微生物还原1,1,1-三氟丙酮来制备对映体过量> 99％的S-1,1,1-三氟-2-丙醇的可扩展的生物催化方法。