公开(公告)号：US12084705B2

公开(公告)日：2024-09-10

申请号：US16375824

申请日：2019-04-04

申请人： United States of America as Represented by the Administrator of the NASA

发明人： Jonathan Matthew Galazka ,  Asif Rahman ,  Samantha Therese Fleury

IPC分类号： C12N9/04 ,  C12N1/16 ,  C12N9/02 ,  C12N15/70 ,  C12P7/00 ,  C12P7/04

CPC分类号： C12P7/04 ,  C12N1/16 ,  C12N9/0071 ,  C12Y114/13025

摘要： Methylotrophic microorganisms, particularly methylotrophic yeasts and more particularly Pichia pastoris, which exhibit the ability to oxidize methane to methanol. Methods of making such microorganisms and DNA constructs for making such microorganisms. Such methylotrophic microorganisms are genetically transformed to exhibit the oxidizing activity of a soluble methane monooxygenase of a methanotrophic bacterium. Such transformed methylotrophic microorganisms contain at least three methane monooxygenase hydroxylase (MMOH) protein subunits of a methanotrophic bacterium: MMOH alpha, MMOH beta and MMOH gamma and a methane monooxygenase reductase (MMOR) of a methanotrophic bacterium.

公开(公告)号：US12084649B2

公开(公告)日：2024-09-10

申请号：US17587006

申请日：2022-01-28

申请人： CORNELL UNIVERSITY

发明人： Stephane C. Corgie ,  Xiaonan Duan ,  Emmanuel Giannelis ,  Daniel Aneshansley ,  Larry P. Walker

IPC分类号： C12N11/04 ,  A62D3/02 ,  B01J8/00 ,  B01J19/12 ,  B01J31/00 ,  B01J31/02 ,  B01J35/33 ,  B01J35/56 ,  B01J37/02 ,  C02F1/48 ,  C02F3/00 ,  C02F3/34 ,  C07C1/12 ,  C07D301/22 ,  C07G1/00 ,  C08F14/00 ,  C08F18/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/08 ,  C12N11/08 ,  C12N11/091 ,  C12N11/098 ,  C12N11/14 ,  C12N13/00 ,  C12P7/04 ,  C12P7/22 ,  C12P17/02 ,  B01J35/64 ,  C02F101/32

CPC分类号： C12N11/04 ,  A62D3/02 ,  B01J8/00 ,  B01J19/12 ,  B01J31/003 ,  B01J31/02 ,  B01J35/33 ,  B01J35/56 ,  B01J37/02 ,  C02F1/484 ,  C02F3/00 ,  C02F3/342 ,  C07C1/12 ,  C07D301/22 ,  C07G1/00 ,  C08F14/00 ,  C08F18/00 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0061 ,  C12N9/0065 ,  C12N11/08 ,  C12N11/091 ,  C12N11/098 ,  C12N11/14 ,  C12N13/00 ,  C12P7/04 ,  C12P7/22 ,  C12P17/02 ,  B01J35/647 ,  B01J35/651 ,  B01J2208/00805 ,  B01J2219/0854 ,  B01J2219/0862 ,  C02F2101/327 ,  C02F2209/006 ,  C02F2303/04 ,  C02F2305/08 ,  C12Y101/03004 ,  C12Y111/01 ,  Y02P20/50 ,  Y02W10/40

摘要： A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

公开(公告)号：US12077800B2

公开(公告)日：2024-09-03

申请号：US17807309

申请日：2022-06-16

申请人： LanzaTech, Inc.

发明人： Paul Alvin Sechrist ,  Bjorn Daniel Heijstra ,  Joshua Jeremy Conolly

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12P7/00 ,  C12P7/04

CPC分类号： C12P7/04 ,  C12M29/06 ,  C12M29/18 ,  C12M29/24

摘要： The disclosure provides a system and a process for injecting and uniformly distributing a liquid into a fermentation broth in a bioreactor for maintaining stable condition within the fermentation broth. The system includes a downcomer section which contains two or more circular pipe conduits extending from a wall of the downcomer to the center vertical axis line of the downcomer. Liquid injection apertures are arranged along the circular pipe conduit and are non-uniformly spaced apart from the center vertical axis of the downcomer to the wall of the downcomer. The apertures further alternate and are offset between the top and bottom surfaces of the circular pipe conduit. The process of the disclosure involves using the system described above for carrying out a fermentation process for converting a C1-substrate to at least one product such as ethanol.

公开(公告)号：US12071637B2

公开(公告)日：2024-08-27

申请号：US17740550

申请日：2022-05-10

申请人： National University of Singapore

发明人： Kun-Lin Yang ,  Kang Zhou ,  Yonghao Cui

IPC分类号： C12N9/04 ,  C12N1/21 ,  C12N9/20 ,  C12P7/04 ,  C12P7/16

CPC分类号： C12N9/0006 ,  C12N9/20 ,  C12P7/04 ,  C12P7/16

摘要： The present invention relates to a composition or combination for the production of butanol and isopropanol, comprising an acetone-butanol-ethanol (ABE)-producing Clostridium strain and a genetically engineered B. subtilis strain, wherein said genetically engineered B. subtilis strain has been transformed by at least one polynucleotide molecule; the at least one polynucleotide molecule comprising a secondary alcohol dehydrogenase gene operably linked to at least one promoter. The invention also relates to methods of producing butanol and isopropanol in a co-culture, methods of producing butyrate, isopropanol and butanol in a co-culture and methods of producing esters.

公开(公告)号：US20240279690A1

公开(公告)日：2024-08-22

申请号：US18444307

申请日：2024-02-16

申请人： Filament Biosciences, LLC.

发明人： Marcella Roe

IPC分类号： C12P7/16 ,  C12P7/04 ,  C12P17/06 ,  C12P17/10

CPC分类号： C12P7/16 ,  C12P7/04 ,  C12P17/06 ,  C12P17/10

摘要： A chemical extraction method comprising exposing a biomass to a protein-denaturing solution to produce a suspended biomass mixture, separating the solubilized biomass mixture into a supernatant phase and a decolorized biomass phase, hydrolyzing the decolorized biomass phase into a hydrolyzed product, fermenting the hydrolyzed product into an alcoholic solvent, mixing the alcoholic solvent and supernatant phase to produce a product suspension, and fractioning the product suspension into a bottom phase, middle phase, and top phase.

公开(公告)号：US12018310B2

公开(公告)日：2024-06-25

申请号：US17447568

申请日：2021-09-13

申请人： PRECIGEN, INC.

发明人： Xinhua Zhao ,  Tina Huynh ,  Jeffrey Orth ,  Lily Yuin Chao ,  James Kealey

IPC分类号： C12P7/04 ,  C12N1/20 ,  C12N15/74

CPC分类号： C12P7/04 ,  C12N1/20 ,  C12N15/74

摘要： Genetically modified microorganisms that have the ability to convert carbon substrates into chemical products such as 1,4-BDO are disclosed. For example, genetically modified methanotrophs that are capable of generating 1,4-BDO at high titers from a methane source are disclosed. Methods of making these genetically modified microorganisms and methods of using them are also disclosed.

公开(公告)号：US20240191261A1

公开(公告)日：2024-06-13

申请号：US18587305

申请日：2024-02-26

申请人： Firmenich SA

发明人： Fredi Bruhlmann ,  Rebecca Buller

IPC分类号： C12P7/04 ,  C07B31/00 ,  C11B9/00 ,  C12N9/02 ,  C12N9/88 ,  C12P7/24

CPC分类号： C12P7/04 ,  C07B31/00 ,  C11B9/0015 ,  C12N9/004 ,  C12N9/88 ,  C12P7/24

摘要： Described herein are methods of producing hexenols including: a) contacting a hydroperoxide of a polyunsaturated fatty acid with a modified hydroperoxide lyase to form a hexenal; and b) reducing the hexenal to a hexenol in the presence of a hydride donor, a ketoreductase, and a co-factor, where the contacting and reducing steps are carried out at essentially the same time in the substantial absence of baker's yeast.

公开(公告)号：US11987782B2

公开(公告)日：2024-05-21

申请号：US17178488

申请日：2021-02-18

申请人： The Government of the United States of America, as represented by the Secretary of the Navy

发明人： Sarah Glaven ,  Elizabeth Onderko ,  Andrew Maygar ,  Matthew Yates

IPC分类号： C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  C12M1/34 ,  C12M3/00 ,  C12P7/04

CPC分类号： C12M23/44 ,  C12M21/12 ,  C12M23/24 ,  C12M23/58 ,  C12M25/10 ,  C12M29/16 ,  C12M41/28 ,  C12P7/04

摘要： Described herein are biofilm bioreactors for synthesis at the interface between two liquids, and methods of using such bioreactors for the biotransformation of feedstocks into chemical products. Also contemplated is the extraction of such products.

公开(公告)号：US20240043880A1

公开(公告)日：2024-02-08

申请号：US17767808

申请日：2020-09-22

申请人： BioPhero ApS

发明人： Bettina Lorántfy ,  Irina Borodina

IPC分类号： C12P7/04 ,  C12P7/24 ,  C12P7/64 ,  C12N9/10 ,  C12N9/02 ,  C12N1/16

CPC分类号： C12P7/04 ,  C12P7/24 ,  C12P7/64 ,  C12N9/1029 ,  C12N9/001 ,  C12N1/165 ,  C12Y203/01086 ,  C12Y103/03006

摘要： The present invention relates to improved methods for producing a hydrophobic compound, in particular a hydrophobic compound which is a pheromone such as an insect pheromone, in a fermentation process involving cultivation of a microorganism such as a yeast, said microorganism producing said hydrophobic compound, wherein the methods facilitate recovery of the hydrophobic compound from the fermentation broth, increase the titer of the hydrophobic compound and/or increase secretion of the hydrophobic compound from the microorganism.

公开(公告)号：US20240026391A1

公开(公告)日：2024-01-25

申请号：US18043177

申请日：2021-09-01

申请人： Danmarks Tekniske Universitet

发明人： Ivan Pogrebnyakov ,  Alex Toftgaard Nielsen

IPC分类号： C12P7/28 ,  C12P7/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N9/04 ,  C12N15/74

CPC分类号： C12P7/28 ,  C12P7/04 ,  C12N9/1029 ,  C12N9/13 ,  C12N9/16 ,  C12N9/88 ,  C12N9/0006 ,  C12N15/74 ,  C12Y203/01009 ,  C12Y208/03008 ,  C12Y208/03009 ,  C12Y208/03005 ,  C12Y401/01004 ,  C12Y101/0108

摘要： The present invention relates to thermophilic cells and methods for the microbial production of volatile compounds, including acetone, butanone and isopropanol. Also provided are nucleic acid constructs, vectors and host cells useful in such methods.